Rabbit Polyclonal TCP1 delta antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCT4 aa 1-50.
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Publications
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- Chinese medical journal 134:2721-27292021CCT4 suppression inhibits tumor growth in hepatocellular carcinoma by interacting with Cdc20.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Li,Chun-Sheng Liu,Ping Wu,An-Sheng Ling,Qi Pan,Xiao-Ning LiPubMed 34732665
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CCT4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P50991
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Alternative names
CCTD, SRB, CCT4, T-complex protein 1 subunit delta, TCP-1-delta, CCT-delta, Chaperonin containing T-complex polypeptide 1 subunit 4, Stimulator of TAR RNA-binding
Recommended products
Rabbit Polyclonal TCP1 delta antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCT4 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CCT4 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P50991
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCP1 delta also known as CCT4 is a subunit of the chaperonin containing TCP1 complex. This protein has a molecular mass of approximately 60 kDa. It is part of a group of proteins in eukaryotic cells known as group II chaperonins. TCP1 delta is expressed in various tissues with higher levels found in brain testis and ovary. It plays a role in protein folding which is important for proper cellular functions.
- Biological function summary
TCP1 delta functions as part of the chaperonin complex assisting in the folding of cytoskeletal proteins such as actin and tubulin. This complex is critical for maintaining cellular integrity and function by ensuring proper protein configuration. TCP1 delta influences cell cycle regulation and proliferation through its role in folding key components of the cytoskeleton. Formation of this complex occurs in the cytoplasm where it effectively brings together other subunits to undertake its biological roles.
- Pathways
TCP1 delta interacts significantly in the protein folding and cytoskeleton organization pathways. It collaborates with other proteins like CCT1 and CCT5 within the TCP-1 ring complex ensuring accurate protein assembly. The protein folding pathway where TCP1 delta operates is essential for cell cycle progression and maintaining cellular homeostasis while also playing a role in the signal transduction involving the cytoskeleton.
- Associated diseases and disorders
TCP1 delta connections appear in some neurodegenerative diseases and cancer. Abnormal function or expression of TCP1 delta relates to misfolding and aggregation of proteins a hallmark of conditions like Huntington’s disease. Moreover dysregulation of TCP1 delta activity is observed in certain cancers where it might interact with oncogenic proteins affecting cell growth and division. Understanding TCP1 delta's role in these diseases helps in identifying potential therapeutic targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-TCP1 delta antibody (ab264353)
Cells prepared using NETN lysis buffer.
All lanes: Western blot - Anti-TCP1 delta antibody (ab264353) at 0.1 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4: TCMK (Mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5: NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 60 kDa
Exposure time: 10s
Immunoprecipitation - Anti-TCP1 delta antibody (ab264353)
TCP1 delta was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab264353 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab264353 at 0.1 μg/ml.
Lane 1: ab264353 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG.Exposure time: 10 secs.
Cells prepared using NETN lysis buffer.
All lanes: Immunoprecipitation - Anti-TCP1 delta antibody (ab264353)
Predicted band size: 60 kDa
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