AB129072

Anti-TCP1 delta antibody [EPR8495(B)]

Name: Anti-TCP1 delta antibody [EPR8495(B)] (ab129072) | Abcam
Brand: Abcam Limited
SKU: AB129072
Price: 450 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(4 Publications)

Rabbit Recombinant Monoclonal TCP1 delta antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

CCTD, SRB, CCT4, T-complex protein 1 subunit delta, TCP-1-delta, CCT-delta, Chaperonin containing T-complex polypeptide 1 subunit 4, Stimulator of TAR RNA-binding

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (AB129072)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (AB129072)

ab129072 at 1/50 dilution staining TCP1 delta in paraffin-embedded Human stomach tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (AB129072)

All lanes:

Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (ab129072) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

Ramos cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 60 kDa

false

Carrier free

Anti-TCP1 delta antibody [EPR8495(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8495(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCP1 delta also known as CCT4 is a subunit of the chaperonin containing TCP1 complex. This protein has a molecular mass of approximately 60 kDa. It is part of a group of proteins in eukaryotic cells known as group II chaperonins. TCP1 delta is expressed in various tissues with higher levels found in brain testis and ovary. It plays a role in protein folding which is important for proper cellular functions.

Biological function summary

TCP1 delta functions as part of the chaperonin complex assisting in the folding of cytoskeletal proteins such as actin and tubulin. This complex is critical for maintaining cellular integrity and function by ensuring proper protein configuration. TCP1 delta influences cell cycle regulation and proliferation through its role in folding key components of the cytoskeleton. Formation of this complex occurs in the cytoplasm where it effectively brings together other subunits to undertake its biological roles.

Pathways

TCP1 delta interacts significantly in the protein folding and cytoskeleton organization pathways. It collaborates with other proteins like CCT1 and CCT5 within the TCP-1 ring complex ensuring accurate protein assembly. The protein folding pathway where TCP1 delta operates is essential for cell cycle progression and maintaining cellular homeostasis while also playing a role in the signal transduction involving the cytoskeleton.

TCP1 delta connections appear in some neurodegenerative diseases and cancer. Abnormal function or expression of TCP1 delta relates to misfolding and aggregation of proteins a hallmark of conditions like Huntington’s disease. Moreover dysregulation of TCP1 delta activity is observed in certain cancers where it might interact with oncogenic proteins affecting cell growth and division. Understanding TCP1 delta's role in these diseases helps in identifying potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638).

See full target information CCT4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 300:107843 PubMed39357829

2024

N-methyladenosine modification of SLC38A7 promotes cell migration, invasion, oxidative phosphorylation, and mitochondrial function in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Hua,Wei-Jun Hua,Cun-Cheng Feng,Qiu-Wei Zhu

Frontiers in oncology 12:975088 PubMed36185250

2022

Chaperonin containing TCP-1 (CCT/TRiC) is a novel therapeutic and diagnostic target for neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Cox,Daniel Nierenberg,Oscar Camargo,Eunkyung Lee,Amr S Khaled,Joseph Mazar,Rebecca J Boohaker,Tamarah J Westmoreland,Annette R Khaled

Acta pharmaceutica Sinica. B 12:2268-2279 PubMed35646538

2022

Anticarin- shows a promising anti-osteosarcoma effect by specifically inhibiting CCT4 to impair proteostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Gan Wang,Min Zhang,Ping Meng,Chengbo Long,Xiaodong Luo,Xingwei Yang,Yunfei Wang,Zhiye Zhang,James Mwangi,Peter Muiruri Kamau,Zhi Dai,Zunfu Ke,Yi Zhang,Wenlin Chen,Xudong Zhao,Fei Ge,Qiumin Lv,Mingqiang Rong,Dongsheng Li,Yang Jin,Xia Sheng,Ren Lai

Scientific reports 10:798 PubMed31964905

2020

Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anne E Showalter,Ana C Martini,Daniel Nierenberg,Kristen Hosang,Naima Ahmed Fahmi,Priya Gopalan,Amr S Khaled,Wei Zhang,Annette R Khaled

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCP1 delta antibody [EPR8495(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (AB129072)

Western blot - Anti-TCP1 delta antibody [EPR8495(B)] (AB129072)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise