AB225876

Anti-TCP1 epsilon/CCT5 antibody

Name: Anti-TCP1 epsilon/CCT5 antibody (ab225876) | Abcam
Brand: Abcam Limited
SKU: AB225876

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(1 Publication)

Rabbit Polyclonal TCP1 epsilon/CCT5 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human CCT5 aa 100-150.

View Alternative Names

CCTE, KIAA0098, CCT5, T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining. Counterstain : hematoxylin (blue).

Supplier Data

Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

TCP1 epsilon/CCT5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225876 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225876 at 0.4 μg/ml.

Lane 1 : ab225876 IP in HEK-293T whole cell lysate.

Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (ab225876)

Predicted band size: 60 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection : DAB staining. Counterstain : hematoxylin (blue).

Supplier Data

Western blot - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

All lanes:

Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab225876) at 0.04 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 60 kDa

true

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human CCT5 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P48643

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab225876 was affinity purified using an epitope specific to TCP1 epsilon/CCT5 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCP1 epsilon also known as CCT5 is an important component of the chaperonin-containing TCP1 complex. This protein weighs around 61 kDa and is expressed in a variety of tissues with higher prevalence in areas with significant protein synthesis and folding activities like the brain and testis. Mechanically TCP1 epsilon facilitates the folding of newly synthesized and stress-denatured proteins by providing an optimal environment for proper protein conformation. It does this by assembling into a barrel-shaped structure that encapsulates the substrate proteins.

Biological function summary

CCT5 is part of the cytosolic chaperonin complex called the TRiC (TCP1 Ring Complex) which is essential for the folding of actin and tubulin. This complex ensures that cytoskeletal proteins achieve their correct tertiary structures necessary for maintaining cellular architecture and function. Additionally this complex prevents the misfolding and aggregation of proteins a process that helps maintain cellular homeostasis and prevent cellular stress.

Pathways

CCT5 plays a significant role in the cytoskeleton modeling and protein homeostasis pathways. In the cytoskeleton pathway CCT5's interaction with actin and tubulin is vital ensuring these proteins attain functional conformation. In the protein homeostasis pathway CCT5 assists in degrading misfolded proteins working closely with other components like heat shock proteins (HSPs) to manage cellular protein load effectively. This interaction highlights its importance in preventing the accumulation of damaged proteins.

Mutations in CCT5 have been linked to some neurodegenerative conditions like Hereditary Sensory Neuropathy Type 2 (HSN2). The failure of CCT5 to assist in proper protein folding can lead to neuronal damage owing to protein aggregates. Additionally disruptions in CCT5 are associated with some forms of cancer where abnormal protein folding influences cell proliferation. CCT5 work in conjunction with other proteins like p53 to maintain cellular function and dysregulation in cancer highlights its role in cellular growth and apoptosis regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638).

See full target information CCT5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 22:640-656 PubMed33230463

2020

TNFAIP8 Promotes Cisplatin Chemoresistance in Triple-Negative Breast Cancer by Repressing p53-Mediated miR-205-5p Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Yu Ma,Yang Li,Hui-Zi Yin,Hang Yin,Yuan-Yuan Qu,Qing-Yong Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCP1 epsilon/CCT5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

Western blot - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise