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Rabbit Polyclonal TCP1 epsilon/CCT5 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human CCT5 aa 100-150.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (AB225876), expandable thumbnail
  • Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (AB225876), expandable thumbnail
  • Western blot - Anti-TCP1 epsilon/CCT5 antibody (AB225876), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (AB225876), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human CCT5 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P48643

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWB
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Predicted
Predicted
Predicted
Orangutan
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100.00000 - 1/500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

2.00000-10.00000 µg/mg of lysate

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Orangutan

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/2000.00000 - 1/10000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Orangutan

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Alternative names

Recommended products

Rabbit Polyclonal TCP1 epsilon/CCT5 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human CCT5 aa 100-150.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human CCT5 aa 100-150. The exact immunogen used to generate this antibody is proprietary information. Database link P48643
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab225876 was affinity purified using an epitope specific to TCP1 epsilon/CCT5 immobilized on solid support.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TCP1 epsilon also known as CCT5 is an important component of the chaperonin-containing TCP1 complex. This protein weighs around 61 kDa and is expressed in a variety of tissues with higher prevalence in areas with significant protein synthesis and folding activities like the brain and testis. Mechanically TCP1 epsilon facilitates the folding of newly synthesized and stress-denatured proteins by providing an optimal environment for proper protein conformation. It does this by assembling into a barrel-shaped structure that encapsulates the substrate proteins.

Biological function summary

CCT5 is part of the cytosolic chaperonin complex called the TRiC (TCP1 Ring Complex) which is essential for the folding of actin and tubulin. This complex ensures that cytoskeletal proteins achieve their correct tertiary structures necessary for maintaining cellular architecture and function. Additionally this complex prevents the misfolding and aggregation of proteins a process that helps maintain cellular homeostasis and prevent cellular stress.

Pathways

CCT5 plays a significant role in the cytoskeleton modeling and protein homeostasis pathways. In the cytoskeleton pathway CCT5's interaction with actin and tubulin is vital ensuring these proteins attain functional conformation. In the protein homeostasis pathway CCT5 assists in degrading misfolded proteins working closely with other components like heat shock proteins (HSPs) to manage cellular protein load effectively. This interaction highlights its importance in preventing the accumulation of damaged proteins.

Associated diseases and disorders

Mutations in CCT5 have been linked to some neurodegenerative conditions like Hereditary Sensory Neuropathy Type 2 (HSN2). The failure of CCT5 to assist in proper protein folding can lead to neuronal damage owing to protein aggregates. Additionally disruptions in CCT5 are associated with some forms of cancer where abnormal protein folding influences cell proliferation. CCT5 work in conjunction with other proteins like p53 to maintain cellular function and dysregulation in cancer highlights its role in cellular growth and apoptosis regulation.

Product promise

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab225876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab225876)

    Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).

  • Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (ab225876), expandable thumbnail

    Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (ab225876)

    TCP1 epsilon/CCT5 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225876 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225876 at 0.4 μg/ml.

    Lane 1: ab225876 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with exposure time of 1 second.

    All lanes: Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (ab225876)

    Predicted band size: 60 kDa

  • Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab225876), expandable thumbnail

    Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab225876)

    All lanes: Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab225876) at 0.04 µg/mL

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg

    Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Lane 4: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

    Lane 5: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 60 kDa

    Exposure time: 3s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab225876), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (ab225876)

    Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for TCP1 epsilon/CCT5 using ab225876 at 1/200 dilution in immunohistochemical analysis. Detection: DAB staining. Counterstain: hematoxylin (blue).

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Product protocols

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