JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB96344

Anti-TCP1 epsilon/CCT5 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal TCP1 epsilon/CCT5 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CCT5 aa 50-350.

View Alternative Names

CCTE, KIAA0098, CCT5, T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-TCP1 epsilon/CCT5 antibody (AB96344)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 epsilon/CCT5 antibody (AB96344)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96344 at 1/200 dilution. Lower image is merged with DNA probe.

Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (AB96344)
  • IP

Supplier Data

Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (AB96344)

Immunoprecipitation of TCP1 epsilon protein from 293T whole cell extracts using 5 µg of ab96344. Western blot analysis was performed using ab96344.

All lanes:

Immunoprecipitation - Anti-TCP1 epsilon/CCT5 antibody (ab96344) at 5 µg

Lane 1:

293T whole cell extracts (Input)

Lane 2:

293T whole cell extracts with control IgG (-)

Lane 3:

293T whole cell extracts (+)

Predicted band size: 60 kDa

false

Western blot - Anti-TCP1 epsilon/CCT5 antibody (AB96344)
  • WB

Supplier Data

Western blot - Anti-TCP1 epsilon/CCT5 antibody (AB96344)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab96344) at 1/6000 dilution

Lane 1:

293T cell lysates at 30 µg

Lane 2:

TCP1 shRNA transfected 293T cell lysates at 30 µg

Predicted band size: 60 kDa

false

Western blot - Anti-TCP1 epsilon/CCT5 antibody (AB96344)
  • WB

Unknown

Western blot - Anti-TCP1 epsilon/CCT5 antibody (AB96344)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TCP1 epsilon/CCT5 antibody (ab96344) at 1/1000 dilution

All lanes:

Molt-4 whole cell lysate at 30 µg

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, IP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CCT5 aa 50-350. The exact immunogen used to generate this antibody is proprietary information.

P48643

style
left-column

Alternative Products

Primary Antibodies

AB225876

Anti-TCP1 epsilon/CCT5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody (AB225876)

  • 0
  • 0
Primary Antibodies

AB129016

Anti-TCP1 epsilon/CCT5 antibody [EPR7562]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)

  • 5
  • 1

Complementary Products

Primary Antibodies

AB92746

Anti-CCT2 antibody [EPR4084]

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CCT2 antibody [EPR4084] (AB92746)

  • 5
  • 2
Primary Antibodies

AB129072

Anti-TCP1 delta antibody [EPR8495(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 delta antibody [EPR8495(B)] (AB129072)

  • 5
  • 1
Primary Antibodies

AB284847

Anti-RFC4 antibody [EP14557] - BSA and Azide free

RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-RFC4 antibody [EP14557] - BSA and Azide free (AB284847)

  • 0
  • 0
Primary Antibodies

AB225878

Anti-CCT3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCT3 antibody (AB225878)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCP1 epsilon also known as CCT5 is an important component of the chaperonin-containing TCP1 complex. This protein weighs around 61 kDa and is expressed in a variety of tissues with higher prevalence in areas with significant protein synthesis and folding activities like the brain and testis. Mechanically TCP1 epsilon facilitates the folding of newly synthesized and stress-denatured proteins by providing an optimal environment for proper protein conformation. It does this by assembling into a barrel-shaped structure that encapsulates the substrate proteins.
Biological function summary

CCT5 is part of the cytosolic chaperonin complex called the TRiC (TCP1 Ring Complex) which is essential for the folding of actin and tubulin. This complex ensures that cytoskeletal proteins achieve their correct tertiary structures necessary for maintaining cellular architecture and function. Additionally this complex prevents the misfolding and aggregation of proteins a process that helps maintain cellular homeostasis and prevent cellular stress.

Pathways

CCT5 plays a significant role in the cytoskeleton modeling and protein homeostasis pathways. In the cytoskeleton pathway CCT5's interaction with actin and tubulin is vital ensuring these proteins attain functional conformation. In the protein homeostasis pathway CCT5 assists in degrading misfolded proteins working closely with other components like heat shock proteins (HSPs) to manage cellular protein load effectively. This interaction highlights its importance in preventing the accumulation of damaged proteins.

Mutations in CCT5 have been linked to some neurodegenerative conditions like Hereditary Sensory Neuropathy Type 2 (HSN2). The failure of CCT5 to assist in proper protein folding can lead to neuronal damage owing to protein aggregates. Additionally disruptions in CCT5 are associated with some forms of cancer where abnormal protein folding influences cell proliferation. CCT5 work in conjunction with other proteins like p53 to maintain cellular function and dysregulation in cancer highlights its role in cellular growth and apoptosis regulation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638).
See full target information CCT5
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 12:2876-87 PubMed23966160

2013

Protein phosphatase 4 is phosphorylated and inactivated by Cdk in response to spindle toxins and interacts with γ-tubulin.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Voss,Kathryn Campbell,Nastja Saranzewa,David G Campbell,C James Hastie,Mark W Peggie,Cristina Martin-Granados,Alan R Prescott,Patricia T W Cohen
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com