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AB129016

Anti-TCP1 epsilon/CCT5 antibody [EPR7562]

5

(1 Review)

|

(10 Publications)

Rabbit Recombinant Monoclonal TCP1 epsilon/CCT5 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

View Alternative Names

CCTE, KIAA0098, CCT5, T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)

ab129016 at 1/100 dilution staining TCP1 epsilon/CCT5 in paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)

ab129016 at 1/100 dilution staining TCP1 epsilon/CCT5 in paraffin-embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)
  • WB

Unknown

Western blot - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)

All lanes:

Western blot - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (ab129016) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MOLT4 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

OI-RD Scanning - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (AB129016)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7562

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCP1 epsilon also known as CCT5 is an important component of the chaperonin-containing TCP1 complex. This protein weighs around 61 kDa and is expressed in a variety of tissues with higher prevalence in areas with significant protein synthesis and folding activities like the brain and testis. Mechanically TCP1 epsilon facilitates the folding of newly synthesized and stress-denatured proteins by providing an optimal environment for proper protein conformation. It does this by assembling into a barrel-shaped structure that encapsulates the substrate proteins.
Biological function summary

CCT5 is part of the cytosolic chaperonin complex called the TRiC (TCP1 Ring Complex) which is essential for the folding of actin and tubulin. This complex ensures that cytoskeletal proteins achieve their correct tertiary structures necessary for maintaining cellular architecture and function. Additionally this complex prevents the misfolding and aggregation of proteins a process that helps maintain cellular homeostasis and prevent cellular stress.

Pathways

CCT5 plays a significant role in the cytoskeleton modeling and protein homeostasis pathways. In the cytoskeleton pathway CCT5's interaction with actin and tubulin is vital ensuring these proteins attain functional conformation. In the protein homeostasis pathway CCT5 assists in degrading misfolded proteins working closely with other components like heat shock proteins (HSPs) to manage cellular protein load effectively. This interaction highlights its importance in preventing the accumulation of damaged proteins.

Mutations in CCT5 have been linked to some neurodegenerative conditions like Hereditary Sensory Neuropathy Type 2 (HSN2). The failure of CCT5 to assist in proper protein folding can lead to neuronal damage owing to protein aggregates. Additionally disruptions in CCT5 are associated with some forms of cancer where abnormal protein folding influences cell proliferation. CCT5 work in conjunction with other proteins like p53 to maintain cellular function and dysregulation in cancer highlights its role in cellular growth and apoptosis regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638).
See full target information CCT5
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature 637:983-990 PubMed39663456

2024

In situ analysis reveals the TRiC duty cycle and PDCD5 as an open-state cofactor.

Applications

Unspecified application

Species

Unspecified reactive species

Huaipeng Xing,Remus R E Rosenkranz,Piere Rodriguez-Aliaga,Ting-Ting Lee,Tomáš Majtner,Stefanie Böhm,Beata Turoňová,Judith Frydman,Martin Beck

Scientific reports 14:4558 PubMed38402348

2024

Biomarker screening using integrated bioinformatics for the development of "normal-impaired glucose intolerance-type 2 diabetes mellitus".

Applications

Unspecified application

Species

Unspecified reactive species

Dongqiang Luo,Xiaolu Gao,Xianqiong Zhu,Jiongbo Xu,Pengfei Gao,Jiayi Zou,Qiaoming Fan,Ying Xu,Tian Liu

Translational research : the journal of laboratory and clinical medicine 260:46-60 PubMed37353110

2023

β-catenin/CBP activation of mTORC1 signaling promotes partial epithelial-mesenchymal states in head and neck cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Eric R Reed,Stacy A Jankowski,Anthony J Spinella,Vikki Noonan,Robert Haddad,Kenichi Nomoto,Junji Matsui,Manish V Bais,Xaralabos Varelas,Maria A Kukuruzinska,Stefano Monti

Cell 185:4770-4787.e20 PubMed36493755

2022

Structural visualization of the tubulin folding pathway directed by human chaperonin TRiC/CCT.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Gestaut,Yanyan Zhao,Junsun Park,Boxue Ma,Alexander Leitner,Miranda Collier,Grigore Pintilie,Soung-Hun Roh,Wah Chiu,Judith Frydman

Frontiers in oncology 12:975088 PubMed36185250

2022

Chaperonin containing TCP-1 (CCT/TRiC) is a novel therapeutic and diagnostic target for neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda Cox,Daniel Nierenberg,Oscar Camargo,Eunkyung Lee,Amr S Khaled,Joseph Mazar,Rebecca J Boohaker,Tamarah J Westmoreland,Annette R Khaled

iScience 25:104289 PubMed35573189

2022

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mio Iwasaki,Yuka Kawahara,Chikako Okubo,Tatsuya Yamakawa,Michiko Nakamura,Tsuyoshi Tabata,Yohei Nishi,Megumi Narita,Akira Ohta,Hirohide Saito,Takuya Yamamoto,Masato Nakagawa,Shinya Yamanaka,Kazutoshi Takahashi

Scientific reports 11:13084 PubMed34158536

2021

Native mass spectrometry analyses of chaperonin complex TRiC/CCT reveal subunit N-terminal processing and re-association patterns.

Applications

Unspecified application

Species

Unspecified reactive species

Miranda P Collier,Karen Betancourt Moreira,Kathy H Li,Yu-Chan Chen,Daniel Itzhak,Rahul Samant,Alexander Leitner,Alma Burlingame,Judith Frydman

Scientific reports 10:798 PubMed31964905

2020

Investigating Chaperonin-Containing TCP-1 subunit 2 as an essential component of the chaperonin complex for tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Anne E Showalter,Ana C Martini,Daniel Nierenberg,Kristen Hosang,Naima Ahmed Fahmi,Priya Gopalan,Amr S Khaled,Wei Zhang,Annette R Khaled

Nature metabolism 1:996-1008 PubMed32694842

2019

1-Deoxydihydroceramide causes anoxic death by impairing chaperonin-mediated protein folding.

Applications

Unspecified application

Species

Unspecified reactive species

J Thomas Hannich,A Galih Haribowo,Sébastien Gentina,Melanie Paillard,Ludovic Gomez,Bruno Pillot,Hélène Thibault,Daniel Abegg,Nicolas Guex,Andreas Zumbuehl,Alexander Adibekian,Michel Ovize,Jean-Claude Martinou,Howard Riezman

Nature microbiology 3:481-493 PubMed29531365

2018

The TRiC chaperonin controls reovirus replication through outer-capsid folding.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan J Knowlton,Isabel Fernández de Castro,Alison W Ashbrook,Daniel R Gestaut,Paula F Zamora,Joshua A Bauer,J Craig Forrest,Judith Frydman,Cristina Risco,Terence S Dermody
View all publications
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Product promise

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