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AB248261

Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free

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Rabbit Recombinant Monoclonal TCP1 epsilon/CCT5 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

CCTE, KIAA0098, CCT5, T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, Chaperonin containing T-complex polypeptide 1 subunit 5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free (AB248261)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free (AB248261)

This data was developed using ab129016, the same antibody clone in a different buffer formulation.

ab129016, at 1/100 dilution, staining TCP1 epsilon/CCT5 in paraffin-embedded Human colon tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free (AB248261)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free (AB248261)

This data was developed using ab129016, the same antibody clone in a different buffer formulation.

ab129016, at 1/100 dilution, staining TCP1 epsilon/CCT5 in paraffin-embedded Human breast tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free (AB248261)
  • WB

Unknown

Western blot - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free (AB248261)

This data was developed using ab129016, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] (<a href='/en-us/products/primary-antibodies/tcp1-epsilon-cct5-antibody-epr7562-ab129016'>ab129016</a>) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MOLT4 cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

OI-RD Scanning - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free (AB248261)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TCP1 epsilon/CCT5 antibody [EPR7562] - BSA and Azide free (AB248261)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7562

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab248261 is the carrier-free version of ab129016.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCP1 epsilon also known as CCT5 is an important component of the chaperonin-containing TCP1 complex. This protein weighs around 61 kDa and is expressed in a variety of tissues with higher prevalence in areas with significant protein synthesis and folding activities like the brain and testis. Mechanically TCP1 epsilon facilitates the folding of newly synthesized and stress-denatured proteins by providing an optimal environment for proper protein conformation. It does this by assembling into a barrel-shaped structure that encapsulates the substrate proteins.
Biological function summary

CCT5 is part of the cytosolic chaperonin complex called the TRiC (TCP1 Ring Complex) which is essential for the folding of actin and tubulin. This complex ensures that cytoskeletal proteins achieve their correct tertiary structures necessary for maintaining cellular architecture and function. Additionally this complex prevents the misfolding and aggregation of proteins a process that helps maintain cellular homeostasis and prevent cellular stress.

Pathways

CCT5 plays a significant role in the cytoskeleton modeling and protein homeostasis pathways. In the cytoskeleton pathway CCT5's interaction with actin and tubulin is vital ensuring these proteins attain functional conformation. In the protein homeostasis pathway CCT5 assists in degrading misfolded proteins working closely with other components like heat shock proteins (HSPs) to manage cellular protein load effectively. This interaction highlights its importance in preventing the accumulation of damaged proteins.

Mutations in CCT5 have been linked to some neurodegenerative conditions like Hereditary Sensory Neuropathy Type 2 (HSN2). The failure of CCT5 to assist in proper protein folding can lead to neuronal damage owing to protein aggregates. Additionally disruptions in CCT5 are associated with some forms of cancer where abnormal protein folding influences cell proliferation. CCT5 work in conjunction with other proteins like p53 to maintain cellular function and dysregulation in cancer highlights its role in cellular growth and apoptosis regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed : 20080638).
See full target information CCT5
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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