AB237762

Anti-TCP1 eta antibody

Name: Anti-TCP1 eta antibody (ab237762) | Abcam
Brand: Abcam Limited
SKU: AB237762

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(1 Publication)

Rabbit Polyclonal TCP1 eta antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CCT7 aa 200 to C-terminus.

View Alternative Names

CCTH, NIP7-1, CCT7, T-complex protein 1 subunit eta, TCP-1-eta, CCT-eta, Chaperonin containing T-complex polypeptide 1 subunit 7, HIV-1 Nef-interacting protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody (AB237762)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody (AB237762)

MCF7 (human breast adenocarcinoma cell line) cells stained for TCP1 eta using ab237762 at 1/100 dilution in ICC/IF.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the primary antibody overnight at 4°C. Secondary used is an Alexa-Fluor^®488-conjugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 eta antibody (AB237762)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 eta antibody (AB237762)

Paraffin-embedded human glioma tissue stained for TCP1 eta with ab237762 at 1/100 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CCT7 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99832

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity greater than 95%.

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCP1 eta protein alternatively known as CCT7 is a molecular chaperone involved in protein folding. It is a component of the eukaryotic chaperonin complex known as the T-complex protein 1 (TCP1) ring complex (TRiC). The protein has an approximate mass of 58 kDa and is widely expressed across various tissues. TCP1 eta forms a multi-subunit structure in cells where it assists in the proper folding of newly synthesized polypeptides particularly actin and tubulin.

Biological function summary

TCP1 eta functions as part of the chaperonin-containing TCP1 (CCT) complex. The CCT complex plays a critical role in maintaining cellular proteostasis by promoting the correct folding and assembly of proteins. Ths complex is key for regulatory processes in the cytoskeleton and cell cycle. The folding activity of TCP1 eta is essential for cytoskeletal dynamics given its involvement with structural proteins like actin.

Pathways

TCP1 eta engages significantly in the actin and tubulin folding pathways. These pathways are vital for the formation of the cytoskeleton and spindle apparatus which are necessary for cell shape and division. TCP1 eta interacts with other chaperonin proteins such as TCP1 epsilon within these pathways to ensure efficient folding and stability of target proteins.

TCP1 eta has been linked to certain neurodegenerative diseases and cancer. Misfolded proteins are often associated with conditions like Huntington's disease where impaired CCT function disrupts protein homeostasis. Additionally there are connections between TCP1 eta and the stability of oncogenic proteins implicating its role in cancer progression. Interactions with other proteins such as p53 are relevant for understanding its role in these disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444).

See full target information CCT7

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 22:e14009 PubMed37960952

2023

3D reconstruction of murine mitochondria reveals changes in structure during aging linked to the MICOS complex.

Applications

Unspecified application

Species

Unspecified reactive species

Zer Vue,Edgar Garza-Lopez,Kit Neikirk,Prasanna Katti,Larry Vang,Heather Beasley,Jianqiang Shao,Andrea G Marshall,Amber Crabtree,Alexandria C Murphy,Brenita C Jenkins,Praveena Prasad,Chantell Evans,Brittany Taylor,Margaret Mungai,Mason Killion,Dominique Stephens,Trace A Christensen,Jacob Lam,Benjamin Rodriguez,Mark A Phillips,Nastaran Daneshgar,Ho-Jin Koh,Alice Koh,Jamaine Davis,Nina Devine,Mohammad Saleem,Estevão Scudese,Kenneth Ryan Arnold,Valeria Vanessa Chavarin,Ryan Daniel Robinson,Moumita Chakraborty,Jennifer A Gaddy,Mariya T Sweetwyne,Genesis Wilson,Elma Zaganjor,James Kezos,Cristiana Dondi,Anilkumar K Reddy,Brian Glancy,Annet Kirabo,Anita M Quintana,Dao-Fu Dai,Karen Ocorr,Sandra A Murray,Steven M Damo,Vernat Exil,Blake Riggs,Bret C Mobley,Jose A Gomez,Melanie R McReynolds,Antentor Hinton

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCP1 eta antibody

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody (AB237762)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCP1 eta antibody (AB237762)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise