Anti-TCP1 eta antibody

• IP

Supplier Data

Immunoprecipitation - Anti-TCP1 eta antibody (AB240566)

TCP1 eta was immunoprecipitated from of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) using ab240566 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab240566 at 0.4 μg/mL.

Lane 1 : ab240566 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-TCP1 eta antibody (ab240566)

Predicted band size: 59 kDa

false

• WB

Supplier Data

Western blot - Anti-TCP1 eta antibody (AB240566)

All lanes:

Western blot - Anti-TCP1 eta antibody (ab240566) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 59 kDa

true

Exposure time: 3s