Skip to main content

Rabbit Recombinant Monoclonal TCP1 eta antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861), expandable thumbnail
  • Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861), expandable thumbnail
  • Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Not recommended
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Not recommended
Predicted
Not recommended
Predicted
Rat
Not recommended
Not recommended
Predicted
Not recommended
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). The TRiC complex plays a role in the folding of actin and tubulin (Probable).

Alternative names

CCTH, NIP7-1, CCT7, T-complex protein 1 subunit eta, TCP-1-eta, CCT-eta, Chaperonin containing T-complex polypeptide 1 subunit 7, HIV-1 Nef-interacting protein

Recommended products

Rabbit Recombinant Monoclonal TCP1 eta antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR10945(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The TCP1 eta protein alternatively known as CCT7 is a molecular chaperone involved in protein folding. It is a component of the eukaryotic chaperonin complex known as the T-complex protein 1 (TCP1) ring complex (TRiC). The protein has an approximate mass of 58 kDa and is widely expressed across various tissues. TCP1 eta forms a multi-subunit structure in cells where it assists in the proper folding of newly synthesized polypeptides particularly actin and tubulin.

Biological function summary

TCP1 eta functions as part of the chaperonin-containing TCP1 (CCT) complex. The CCT complex plays a critical role in maintaining cellular proteostasis by promoting the correct folding and assembly of proteins. Ths complex is key for regulatory processes in the cytoskeleton and cell cycle. The folding activity of TCP1 eta is essential for cytoskeletal dynamics given its involvement with structural proteins like actin.

Pathways

TCP1 eta engages significantly in the actin and tubulin folding pathways. These pathways are vital for the formation of the cytoskeleton and spindle apparatus which are necessary for cell shape and division. TCP1 eta interacts with other chaperonin proteins such as TCP1 epsilon within these pathways to ensure efficient folding and stability of target proteins.

Associated diseases and disorders

TCP1 eta has been linked to certain neurodegenerative diseases and cancer. Misfolded proteins are often associated with conditions like Huntington's disease where impaired CCT function disrupts protein homeostasis. Additionally there are connections between TCP1 eta and the stability of oncogenic proteins implicating its role in cancer progression. Interactions with other proteins such as p53 are relevant for understanding its role in these disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10945(B)] (ab170861), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10945(B)] (ab170861)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling TCP1 eta with ab170861 at a 1/100 dilution (green) DAPI nuclear staining (blue).

  • Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (ab170861), expandable thumbnail

    Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (ab170861)

    All lanes: Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (ab170861) at 1/10000 dilution

    Lane 1: Human testis lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

    Lane 4: HeLa cell lysate at 10 µg

    Predicted band size: 59 kDa

  • Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (ab170861), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (ab170861)

    TCP1 eta western blot using anti-TCP1 eta antibody [EPR10945(B)] ab170861. Publication image and figure legend from Noormohammadi, A., Khodakarami, A., et al., 2016, Nat Commun, PubMed 27892468.


    ab170861 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab170861 please see the product overview.

    Knockdown of CCT subunits affects pluripotency of hESCs.(a) Real-time PCR analysis of pluripotency markers in H9 hESCs. Graphs (relative expression to NT shRNA) represent the mean±s.e.m. of at least three independent experiments. Knockdown of CCT8 (n=3), CCT2 (n=3), CCT6A (n=4) and CCT7 (n=4) decrease the expression of pluripotency markers. (b) Knockdown of CCT7 induces a decrease in OCT4 protein levels. β-actin is the loading control. The images are representative of three independent experiments. (c) Real-time PCR analysis of germ-layer markers in H9 hESCs (relative expression to NT shRNA). Graph represents the mean±s.e.m. of four independent experiments. All the statistical comparisons were made by Student's t test for unpaired samples. P value: *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com