AB170861

Anti-TCP1 eta antibody [EPR10945(B)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal TCP1 eta antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CCTH, NIP7-1, CCT7, T-complex protein 1 subunit eta, TCP-1-eta, CCT-eta, Chaperonin containing T-complex polypeptide 1 subunit 7, HIV-1 Nef-interacting protein

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling TCP1 eta with ab170861 at a 1/100 dilution (green) DAPI nuclear staining (blue).

Supplier Data

Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861)

All lanes:

Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (ab170861) at 1/10000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 59 kDa

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CiteAb

Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861)

TCP1 eta western blot using anti-TCP1 eta antibody [EPR10945(B)] ab170861. Publication image and figure legend from Noormohammadi, A., Khodakarami, A., et al., 2016, Nat Commun, PubMed 27892468.

ab170861 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab170861 please see the product overview.

Knockdown of CCT subunits affects pluripotency of hESCs.(a) Real-time PCR analysis of pluripotency markers in H9 hESCs. Graphs (relative expression to NT shRNA) represent the mean±s.e.m. of at least three independent experiments. Knockdown of CCT8 (n=3), CCT2 (n=3), CCT6A (n=4) and CCT7 (n=4) decrease the expression of pluripotency markers. (b) Knockdown of CCT7 induces a decrease in OCT4 protein levels. β-actin is the loading control. The images are representative of three independent experiments. (c) Real-time PCR analysis of germ-layer markers in H9 hESCs (relative expression to NT shRNA). Graph represents the mean±s.e.m. of four independent experiments. All the statistical comparisons were made by Student's t test for unpaired samples. P value : *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

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Carrier free

Anti-TCP1 eta antibody [EPR10945(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10945(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.35% Sodium citrate, 0.17% Sodium chloride, 0.05% BSA, 0.03% EDTA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCP1 eta protein alternatively known as CCT7 is a molecular chaperone involved in protein folding. It is a component of the eukaryotic chaperonin complex known as the T-complex protein 1 (TCP1) ring complex (TRiC). The protein has an approximate mass of 58 kDa and is widely expressed across various tissues. TCP1 eta forms a multi-subunit structure in cells where it assists in the proper folding of newly synthesized polypeptides particularly actin and tubulin.

Biological function summary

TCP1 eta functions as part of the chaperonin-containing TCP1 (CCT) complex. The CCT complex plays a critical role in maintaining cellular proteostasis by promoting the correct folding and assembly of proteins. Ths complex is key for regulatory processes in the cytoskeleton and cell cycle. The folding activity of TCP1 eta is essential for cytoskeletal dynamics given its involvement with structural proteins like actin.

Pathways

TCP1 eta engages significantly in the actin and tubulin folding pathways. These pathways are vital for the formation of the cytoskeleton and spindle apparatus which are necessary for cell shape and division. TCP1 eta interacts with other chaperonin proteins such as TCP1 epsilon within these pathways to ensure efficient folding and stability of target proteins.

TCP1 eta has been linked to certain neurodegenerative diseases and cancer. Misfolded proteins are often associated with conditions like Huntington's disease where impaired CCT function disrupts protein homeostasis. Additionally there are connections between TCP1 eta and the stability of oncogenic proteins implicating its role in cancer progression. Interactions with other proteins such as p53 are relevant for understanding its role in these disease contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of actin, tubulin and other proteins upon ATP hydrolysis (PubMed : 25467444, PubMed : 36493755, PubMed : 35449234, PubMed : 37193829). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed : 25467444).

See full target information CCT7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging cell 20:e13446 PubMed34327811

2021

The intrinsic chaperone network of Arabidopsis stem cells confers protection against proteotoxic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Ernesto Llamas,Salvador Torres-Montilla,Hyun Ju Lee,María Victoria Barja,Elena Schlimgen,Nick Dunken,Prerana Wagle,Wolfgang Werr,Alga Zuccaro,Manuel Rodríguez-Concepción,David Vilchez

Nature communications 7:13649 PubMed27892468

2016

Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.

Applications

Species

Unspecified reactive species

Alireza Noormohammadi,Amirabbas Khodakarami,Ricardo Gutierrez-Garcia,Hyun Ju Lee,Seda Koyuncu,Tim König,Christina Schindler,Isabel Saez,Azra Fatima,Christoph Dieterich,David Vilchez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCP1 eta antibody [EPR10945(B)]

Immunocytochemistry/ Immunofluorescence - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861)

Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861)

Western blot - Anti-TCP1 eta antibody [EPR10945(B)] (AB170861)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise