Rabbit Polyclonal TCP1 theta antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CCT8 aa 450 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CCT8 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P50990
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Cynomolgus monkey, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).
Alternative names
C21orf112, CCTQ, KIAA0002, CCT8, T-complex protein 1 subunit theta, TCP-1-theta, CCT-theta, Chaperonin containing T-complex polypeptide 1 subunit 8, Renal carcinoma antigen NY-REN-15
Recommended products
Rabbit Polyclonal TCP1 theta antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human CCT8 aa 450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: 99% Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CCT8 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P50990
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab176691 was affinity purified using an epitope specific to TCP1 theta immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TCP1 theta also known as CCT8 is a chaperonin protein that plays a critical role in assisting the folding of newly synthesized and stress-denatured proteins. With a molecular mass of approximately 59 kDa TCP1 theta is an essential component of the chaperonin-containing TCP1 (CCT) complex also referred to as the TRiC complex. It is found primarily in the cytoplasm and is expressed in various tissues with high levels noted in the brain liver and testis.
- Biological function summary
TCP1 theta functions within the CCT complex to facilitate the proper folding of proteins particularly actin and tubulin which are vital for maintaining cellular structure and function. By ensuring correct protein folding TCP1 theta helps prevent aggregation that could lead to cellular dysfunction. This chaperonin is part of a large complex that cooperates with other chaperonins to stabilize newly synthesized polypeptides and mediate their conformational maturation.
- Pathways
TCP1 theta is integral to the protein folding pathway and actively participates in cytoskeletal organization. TCP1 theta interacts closely with proteins like actin and tubulin aiding in their folding and stabilization. Its activity supports the function of the cytoskeleton influencing cell shape and transport processes. The protein folding pathway in which TCP1 theta plays an important role is essential for proper cellular function and response to stress.
- Associated diseases and disorders
Malfunction or dysregulation of TCP1 theta contributes to conditions such as cancer and neurodegenerative diseases. In cancer abnormal expression of TCP1 theta may support tumor progression and cell survival by aiding in the folding of proteins required for rapid cell division. Additionally its role in neurodegenerative diseases connects to alterations in protein homeostasis often exacerbating disorders like Alzheimer's disease. Proteins involved in these diseases such as tau in Alzheimer's may rely on TCP1 theta for correct folding linking its function to pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunoprecipitation - Anti-TCP1 theta antibody (ab176691)
Detection of Human TCP1 theta by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab176691 used for IP at 6 μg/mg lysate. For blotting immunoprecipitated TCP1 theta, ab176691 was used at 0.04 μg/ml.
All lanes: Immunoprecipitation - Anti-TCP1 theta antibody (ab176691)
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 3s
Western blot - Anti-TCP1 theta antibody (ab176691)
All lanes: Western blot - Anti-TCP1 theta antibody (ab176691) at 0.04 µg/mL
Lane 1: HeLa lysate at 50 µg
Lane 2: HeLa lysate at 15 µg
Lane 3: 293T lysate at 50 µg
Lane 4: Jurkat lysate at 50 µg
Lane 5: Mouse NIH3T3 lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 60 kDa
Exposure time: 10s
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