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AB167645

Anti-TCR alpha antibody

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Mouse Polyclonal TRAC antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRAC.

View Alternative Names

TCRA, TRAC, T cell receptor alpha chain constant

1 Images
Western blot - Anti-TCR alpha antibody (AB167645)
  • WB

Unknown

Western blot - Anti-TCR alpha antibody (AB167645)

All lanes:

Western blot - Anti-TCR alpha antibody (ab167645) at 1 µg/mL

Lane 1:

TCR alpha transfected 293T cell lysate at 15 µL

Lane 2:

Non-transfected 293T cell lysate at 15 µL

Secondary

All lanes:

Goat Anti-Mouse IgG HRP antibody at 1/2500 dilution

Predicted band size: 16 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TRAC.

P01848

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TCR alpha also known as T cell receptor alpha or alfa TCR is an essential component of the T cell receptor (TCR) complex. It is a transmembrane protein with a typical mass of approximately 50-55 kDa. The TCR alpha chain together with the TCR beta chain forms the heterodimer that is critical for antigen recognition. This receptor complex is expressed on the surface of T cells specifically on mature T cells that have undergone successful TCR rearrangement. The interaction of TCR alpha with major histocompatibility complex (MHC) molecules loaded with antigen peptides leads to T cell activation.
Biological function summary

TCR alpha plays a central role in immune response by recognizing antigens presented by MHC. TCR alpha chain is part of a larger T cell receptor complex which includes the CD3 signaling molecules. The binding of antigens triggers a signal transduction cascade through CD3 components leading to T cell activation proliferation and differentiation into effector T cells. These effector cells then participate in immune defenses against pathogens and are important for immune surveillance. TCR alpha's interaction with the environment and antigens initiates immune synapse formation leading to proper signal transmission.

Pathways

TCR alpha is an important participant in the T cell receptor signaling pathway. This pathway plays an important role in T cell activation and function linking innate and adaptive immunity. TCR alpha interacts with several signaling proteins such as Lck and ZAP-70 which propagate the signal downstream to various transcription factors that modulate gene expression. This cascade affects cellular processes including cytokine production and survival further connecting the TCR signaling pathway to other pathways like the MAP kinase signaling pathway.

TCR alpha has been associated with autoimmune diseases and immunodeficiencies. Alterations in TCR alpha chain can lead to conditions such as systemic lupus erythematosus where an improper response of T cells to self-antigens occurs. Moreover mutations or malfunctions in TCR alpha expression can result in severe combined immunodeficiency (SCID) where patients have a severely compromised immune system. Other proteins such as CD3 and MHC are involved in both autoimmune diseases and immunodeficiencies showing the interconnected nature of immune components and the importance of TCR alpha in maintaining immune homeostasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of T cell receptor (TR) alpha chain (PubMed : 24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed : 25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed : 23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed : 15040585).
See full target information TRAC
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Product promise

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