Anti-TCR alpha antibody [EPR29621-40] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR alpha antibody [EPR29621-40] - BSA and Azide free (AB324535)

This data was developed using ab324527, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TCR alpha with ab324527 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human tonsil.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCR alpha antibody [EPR29621-40] - BSA and Azide free (AB324535)

This data was developed using ab324527, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Jurkat (human T cell leukemia T lymphocyte from peripheral blood) cells labelling TCR alpha with ab324527 at 1/200 (2.4 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in Jurkat cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Negative control : Raji.

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab324527 at 1/200 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR alpha antibody [EPR29621-40] - BSA and Azide free (AB324535)

This data was developed using ab324527, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human Hodgkin lymphoma tissue labeling TCR alpha with ab324527 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human Hodgkin lymphoma.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR alpha antibody [EPR29621-40] - BSA and Azide free (AB324535)

This data was developed using ab324527, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling TCR alpha with ab324527 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in human spleen.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-TCR alpha antibody [EPR29621-40] - BSA and Azide free (AB324535)

This data was developed using ab324527, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : eyeball

The bands beneath the target band (50 kDa) are likely to be degradation products (PMID : 9252124).

The expression profile/ molecular weight observed is consistent with what has been described in the literature(PMID : 12021779, PMID : 11406585)

The identity of the bands higher than 250 kDa in lane 2 is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 10 seconds; Lanes 3-4 : 180 seconds

All lanes:

Western blot - Anti-TCR alpha antibody [EPR29621-40] (<a href='/en-us/products/primary-antibodies/tcr-alpha-antibody-epr29621-40-ab324527'>ab324527</a>) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg

Lane 2:

Human eyeball tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Human lymph node tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 30-50 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-TCR alpha antibody [EPR29621-40] - BSA and Azide free (AB324535)

This data was developed using ab324527, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Raji, Ramos.

The expression profile/ molecular weight observed is consistent with what has been described in the literature(PMID : 12021779, PMID : 11406585)

The bands beneath the target band (50 kDa) are likely to be degradation products (PMID : 9252124).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-TCR alpha antibody [EPR29621-40] (<a href='/en-us/products/primary-antibodies/tcr-alpha-antibody-epr29621-40-ab324527'>ab324527</a>) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 40 µg

Lane 2:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 40 µg

Lane 3:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 40 µg

Lane 4:

HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 40 µg

Lane 5:

H9 (human lymphoma cutaneous T lymphocyte) whole cell lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 30-50 kDa,36 kDa

false

Exposure time: 10s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR alpha antibody [EPR29621-40] - BSA and Azide free (AB324535)

This data was developed using ab324527, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling TCR alpha with ab324527 at 1/500 (0.96 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining in human cerebrum.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins