Armenian hamster Monoclonal TCA antibody. Suitable for IP, Flow Cyt, IF, IHC-Fr and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Mouse Trac.
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate
| IP | Flow Cyt | IF | IHC-Fr | |
|---|---|---|---|---|
| Mouse | Expected | Expected | Expected | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Constant region of T cell receptor (TR) alpha chain. Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens. Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity.
T-cell receptor alpha chain constant, Trac
Armenian hamster Monoclonal TCA antibody. Suitable for IP, Flow Cyt, IF, IHC-Fr and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Mouse Trac.
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate
ab25336 reacts with a common epitope of the ß chain of the mouse aß T cell receptor (TCR) complex.
Purified from tissue culture supernatant
ab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection.
The T-cell receptor (TCR) alpha and TCR beta sometimes referred to as alpha beta TCR or alfa TCR are essential components of the immune response machinery. TCR alpha and beta chains each have a mass that varies between 30-40 kDa depending on the variable region's length. These receptor chains are expressed on the surface of T-cells more specifically on the vast majority of T lymphocytes and play a critical role in antigen recognition. Commercial names like anti-TCR or H57 might refer to antibodies targeting these chains.
TCR alpha and beta chains form a heterodimeric complex with other important proteins like CD3 to transmit signals inside the T-cell upon antigen binding. This complex becomes active when T-cells recognize peptide antigens presented by major histocompatibility complex (MHC) molecules on antigen-presenting cells. The interaction between TCR and MHC-peptide complex initializes T-cell activation and subsequent immune responses being central to adaptive immunity.
TCR alpha and beta are integral to the T-cell receptor signaling pathway. This pathway facilitates the activation of important transcription factors such as NF-kB and AP-1 which drive T-cell proliferation and differentiation. The signaling involves several other proteins such as protein kinase C theta and ZAP-70 which further propagate intracellular signaling cascades essential for a fully functional immune response.
Mutations or malfunctions in TCR alpha and beta chains can associate with autoimmune disorders like rheumatoid arthritis and certain types of immunodeficiency. Rheumatoid arthritis involves aberrant TCR signaling pathways that result in inappropriate immune responses against self-antigens. In some immunodeficiencies defective TCR signaling impairs T-cell development and function potentially involving related proteins like CD3 and ZAP-70 leading to compromised immune defenses.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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