Skip to main content

Rabbit Recombinant Monoclonal TCB1 antibody. Suitable for IHC-P, WB, IP and reacts with Mouse, Rat, Transfected cell lysate - Mouse samples.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-TCR beta antibody [EPR27870-34] (AB313579), expandable thumbnail
  • Western blot - Anti-TCR beta antibody [EPR27870-34] (AB313579), expandable thumbnail
  • Western blot - Anti-TCR beta antibody [EPR27870-34] (AB313579), expandable thumbnail
  • Western blot - Anti-TCR beta antibody [EPR27870-34] (AB313579), expandable thumbnail
  • Western blot - Anti-TCR beta antibody [EPR27870-34] (AB313579), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBIPICC/IFFlow Cyt
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Tested
Tested
Expected
Not recommended
Not recommended
Transfected cell lysate - Mouse
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Transfected cell lysate - Mouse
Dilution info
1/5000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell lysate - Mouse
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Additional Targets

TCB2_MOUSE

Alternative names

T-cell receptor beta-1 chain C region, TCR beta

Recommended products

Rabbit Recombinant Monoclonal TCB1 antibody. Suitable for IHC-P, WB, IP and reacts with Mouse, Rat, Transfected cell lysate - Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27870-34
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TCR beta also known as T-cell receptor beta is an integral component of the T-cell receptor (TCR) complex. It weighs approximately 40-50 kDa and is expressed on the surface of T cells. TCR beta contributes to the recognition of antigens by interacting with peptide fragments presented by major histocompatibility complex (MHC) molecules. This interaction is essential for T-cell activation which plays a significant role in the adaptive immune response.

Biological function summary

TCR beta is part of the T-cell receptor complex. This complex is critical for antigen recognition and T-cell signaling. The complex comprises multiple subunits including CD3 chains that facilitate signal transduction upon antigen binding. This initiates a cascade of intracellular events leading to T-cell activation and proliferation. TCR beta's role in this complex makes it essential for recognizing pathogens and initiating immune responses.

Pathways

TCR beta is involved in T-cell receptor signaling and adaptive immune pathways. It is closely associated with the Zap70 protein which transmits activation signals from the TCR upon antigen recognition. This signaling cascade further activates the MAPK and NF-κB pathways influencing T-cell differentiation and cytokine production. The interaction between TCR beta and these pathways highlights its fundamental role in immune system regulation.

Associated diseases and disorders

Dysregulation of TCR beta can contribute to autoimmune diseases and certain types of cancer. For instance aberrant TCR signaling can lead to autoimmunity where T cells attack the body's own tissues. Additionally in some leukemias mutations or rearrangements involving the TCR beta chain can promote uncontrolled cell proliferation. TCR beta's involvement with HLA proteins in autoimmune conditions showcases its significance in disease development and management.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-TCR beta antibody [EPR27870-34] (ab313579), expandable thumbnail

    Immunoprecipitation - Anti-TCR beta antibody [EPR27870-34] (ab313579)

    TCR beta was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte) whole cell lysate with ab313579 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313579 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: EL4 (mouse lymphoma T lymphocyte) whole cell lysate
    Lane 2: ab313579 IP in EL4 (mouse lymphoma T lymphocyte) whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab313579 in EL4 whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 180 seconds

    All lanes: Immunoprecipitation - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/30 dilution

    All lanes: EL4 (mouse lymphoma T lymphocyte) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579), expandable thumbnail

    Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: A20, skeletal muscle, brain.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 92 seconds

    All lanes: Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/1000 dilution

    Lane 1: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Lane 2: A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate at 20 µg

    Lane 3: Mouse lymph node tissue lysate at 20 µg

    Lane 4: Mouse thymus tissue lysate at 20 µg

    Lane 5: Mouse skeletal muscle tissue lysate at 20 µg

    Lane 6: Mouse brain tissue lysate at 20 µg

    Lane 7: Rat lymph node tissue lysate at 20 µg

    Lane 8: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 37 kDa

    Exposure time: 92s

  • Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579), expandable thumbnail

    Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody recognizes both TCR beta-1 and TCR beta-2.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution, anti-His antibody (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.
    Exposure time: 37 seconds

    All lanes: Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/5000 dilution

    Lane 1: 293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a his tag whole cell lysate at 20 µg

    Lane 2: 293T transfected with a mouse TCR beta-1 expression vector containi a His-tag whole cell lysate at 20 µg

    Lane 3: 293T transfected with a mouse TCR beta-2 expression vector containi a His-tag whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 22 kDa

    Exposure time: 37s

  • Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579), expandable thumbnail

    Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    TCR beta detected a shift from 37kDa to 30kDa band after deglycosylation treatment.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 114 seconds

    All lanes: Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/1000 dilution

    Lane 1: Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Lane 2: EL4 (mouse lymphoma T lymphocyte) whole cell lysate treated with Peptide:N-glycosidase F (PNGase F) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 37 kDa

    Exposure time: 114s

  • Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579), expandable thumbnail

    Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: 92 seconds

    All lanes: Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 20 µg

    Lane 2: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 37 kDa

    Exposure time: 92s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (ab313579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (ab313579)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling TCR beta with ab313579 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining on mouse skeletal muscle.The section was incubated with ab313579 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (ab313579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (ab313579)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling TCR beta with ab313579 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat spleen.The section was incubated with ab313579 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (ab313579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (ab313579)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling TCR beta with ab313579 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on immune cells of mouse colon.The section was incubated with ab313579 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (ab313579), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (ab313579)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TCR beta with ab313579 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse spleen.The section was incubated with ab313579 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com