Anti-TCR beta antibody [EPR27870-34]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (AB313579)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TCR beta with ab313579 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse spleen.The section was incubated with ab313579 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (AB313579)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling TCR beta with ab313579 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining on mouse skeletal muscle.The section was incubated with ab313579 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (AB313579)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling TCR beta with ab313579 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on immune cells of mouse colon.The section was incubated with ab313579 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [EPR27870-34] (AB313579)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling TCR beta with ab313579 at 1/2000 (0.252 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat spleen.The section was incubated with ab313579 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

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Immunoprecipitation - Anti-TCR beta antibody [EPR27870-34] (AB313579)

TCR beta was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte) whole cell lysate with ab313579 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313579 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lane 2 : ab313579 IP in EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313579 in EL4 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds

All lanes:

Immunoprecipitation - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/1000 dilution

All lanes:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-TCR beta antibody [EPR27870-34] (AB313579)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 92 seconds

All lanes:

Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 37 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-TCR beta antibody [EPR27870-34] (AB313579)

Blocking and diluting buffer and concentration : 5% NFDM/TBST TCR beta detected a shift from 37kDa to 30kDa band after deglycosylation treatment. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 114 seconds

All lanes:

Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/1000 dilution

Lane 1:

Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate treated with Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 37 kDa

false

Exposure time: 114s

• WB

Supplier Data

Western blot - Anti-TCR beta antibody [EPR27870-34] (AB313579)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : A20, skeletal muscle, brain. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 92 seconds

All lanes:

Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/1000 dilution

Lane 1:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Lane 2:

A20 (mouse reticum sarcoma B lymphocyte) whole cell lysate at 20 µg

Lane 3:

Mouse lymph node tissue lysate at 20 µg

Lane 4:

Mouse thymus tissue lysate at 20 µg

Lane 5:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Lane 7:

Rat lymph node tissue lysate at 20 µg

Lane 8:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 37 kDa

false

Exposure time: 92s

• WB

Supplier Data

Western blot - Anti-TCR beta antibody [EPR27870-34] (AB313579)

Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody recognizes both TCR beta-1 and TCR beta-2. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution, anti-His antibody (ab213204) staining at 1/5000 dilution. Exposure time : 37 seconds

All lanes:

Western blot - Anti-TCR beta antibody [EPR27870-34] (ab313579) at 1/5000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) transfected with an empty vector containi a his tag whole cell lysate at 20 µg

Lane 2:

293T transfected with a mouse TCR beta-1 expression vector containi a His-tag whole cell lysate at 20 µg

Lane 3:

293T transfected with a mouse TCR beta-2 expression vector containi a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 22 kDa

false

Exposure time: 37s