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AB325979

Anti-TCR beta antibody [SP305] - BSA and Azide free

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Rabbit Recombinant Monoclonal T Cell Receptor antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

T cell receptor beta constant 1, TRBC1, TCR beta, TCRBC2, TRBC2, T cell receptor beta constant 2, TCR beta

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Formalin-fixed paraffin-embedded human colon tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Formalin-fixed paraffin-embedded human tonsil tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Formalin-fixed paraffin-embedded human urinary bladder tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Formalin-fixed paraffin-embedded human thymus tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Formalin-fixed paraffin-embedded human lymph node tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Formalin-fixed paraffin-embedded human stomach tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling TCR beta with ab227699 at 1/100 dilution (2.28 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling TCR beta with ab227699 at 1/100 dilution (2.28 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Formalin-fixed paraffin-embedded human spleen stained for TCR beta using ab227699 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • WB

Lab

Western blot - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Blocking/Diluting buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-TCR beta antibody [SP305] (<a href='/en-us/products/primary-antibodies/trbc1-antibody-sp305-ab227699'>ab227699</a>) at 0.228 µg/mL

Lane 1:

Ramos (Human Burkitt's lymphoma B lymphocyte) cell lysate

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) cell lysate

Lane 3:

HEK-293 ( Human embryonic kidney epithelial cell) cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Predicted band size: 20 kDa

Observed band size: 35 kDa

false

Western blot - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)
  • WB

Lab

Western blot - Anti-TCR beta antibody [SP305] - BSA and Azide free (AB325979)

This data was developed using ab227699, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Exposure Time :
A. 40s
B. 7s
C. 7s

All lanes:

Western blot - Anti-TCR beta antibody [SP305] (<a href='/en-us/products/primary-antibodies/trbc1-antibody-sp305-ab227699'>ab227699</a>) at 1/5000 dilution

Lane 1:

293T+OE-empty at 20 µg

Lanes 2 - 3:

293T+OE-TRBC1 at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP305

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>Primary antibody incubation for 1 hour at room temperature.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval by boiling tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p><p>Primary antibody incubation for 10 minutes at room temperature.</p>" } } }
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Product details

ab325979 is the carrier-free version of ab227699

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRBC1 also known as T-Cell Receptor Beta Constant 1 is involved in the formation of the T-cell receptor complex. It has a molecular mass of around 18 kDa. This protein component is prominently expressed on the surface of T lymphocytes important for mediating immune responses. TRBC1 is a part of the TCR constant region playing a significant role in antigen recognition through its interaction with antigen-presenting cells.
Biological function summary

TRBC1 contributes to the immune system’s mechanism by helping identify foreign invaders in the body. It works as an integral part of the T-cell receptor (TCR) complex facilitating T-cell activation and signaling. The complex formation includes variable and constant regions with TRBC1 as a fixed part of the constant region ensuring the proper receptor structure for effective recognition. TCR assembly is important for T cells to detect specific antigens.

Pathways

TRBC1 participates in the adaptive immune response and T-cell receptor signaling pathway. Interacting proteins such as CD3 and ZAP70 help transmit signals initiated at the surface by antigen recognition leading to downstream effects such as cell proliferation and cytokine release. TRBC1’s involvement in these pathways ensures the T cells' ability to respond to invading pathogens and maintain immune surveillance.

TRBC1 has been implicated in conditions like autoimmune diseases and certain cancers such as T-cell lymphomas. Its interaction with proteins like CD3 impacts these disorders by amplifying or misdirecting immune responses. Aberrant TRBC1 expression or mutations can disrupt normal immune functions triggering disease development or progression. Understanding TRBC1's role in these contexts helps in developing targeted therapies and diagnostic tools.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of T cell receptor (TR) beta chain (PubMed : 24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed : 25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed : 23524462, PubMed : 9382891). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed : 15040585).
See full target information TRBC1

Additional targets

TRBC2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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