AB171088

Anti-TCR beta F1 antibody [8A3]

Name: Anti-TCR beta F1 antibody [8A3] (ab171088) | Abcam
Brand: Abcam Limited
SKU: AB171088
Price: 470 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(1 Publication)

Anti-TCR beta F1 antibody [8A3] (ab171088) is a mouse monoclonal antibody detecting TCR beta F1 in Western Blot, Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse,.

View Alternative Names

T cell receptor beta variable 7-9, TRBV7-9

1 Images

Flow Cytometry - Anti-TCR beta F1 antibody [8A3] (AB171088)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-TCR beta F1 antibody [8A3] (AB171088)

Flow cytometry analysis of TCR beta F1 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR beta F1 monoclonal antibody (ab171088) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8A3

Isotype

IgG1

Carrier free

Reacts with

Human, Mouse, Primates

Applications

Flow Cyt, ICC/IF, IP, IHC-P, WB

applications

Immunogen

Full Length Protein corresponding to Human TRBV7-9. The exact immunogen used to generate this antibody is proprietary information.

P04435

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Reactivity data

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Product details

What is this antibody validated in?
Anti-TCR beta F1 antibody [8A3] (ab171088) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCR beta F1 also known as T-cell receptor beta chain is a component of the T-cell receptor (TCR) complex. It plays a vital role in the immune response. The TCR beta F1 protein has a molecular mass of approximately 45 kDa. It is expressed on the surface of T lymphocytes which are white blood cells involved in the adaptive immune system. This protein is required for recognizing antigens presented by major histocompatibility complex (MHC) molecules on antigen-presenting cells.

Biological function summary

The TCR beta F1 chain forms part of the T-cell receptor complex which is necessary for T-cell activation. The complex also includes other components like TCR alpha chain and CD3 proteins. TCR beta F1 interacts with processed antigens bound to MHC molecules triggering intracellular signaling cascades. These cascades result in T-cell proliferation differentiation and cytokine release essential for the immune defense against pathogens and tumors.

Pathways

TCR beta F1 is central to the T-cell receptor signaling pathway. This pathway initiates after antigen recognition activating downstream signaling molecules such as ZAP70 and LCK. These molecules further propagate signals to pathways like the MAPK/ERK pathway influencing cell survival and proliferation. Additionally TCR beta F1 is part of the NFAT signaling pathway coordinating T-cell activation and cytokine gene transcription.

TCR beta F1 has implications in autoimmune diseases and cancers. In autoimmune diseases like rheumatoid arthritis abnormal TCR signaling leads to excessive immune responses against self-antigens. In certain cancers such as leukemia altered TCR beta chain expressions contribute to uncontrolled T-cell proliferation. These conditions showcase the significant role of TCR beta F1 connecting to proteins like CD3 which also participate in these pathological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

V region of the variable domain of T cell receptor (TR) beta chain that participates in the antigen recognition (PubMed : 24600447). Alpha-beta T cell receptors are antigen specific receptors which are essential to the immune response and are present on the cell surface of T lymphocytes. Recognize peptide-major histocompatibility (MH) (pMH) complexes that are displayed by antigen presenting cells (APC), a prerequisite for efficient T cell adaptive immunity against pathogens (PubMed : 25493333). Binding of alpha-beta TR to pMH complex initiates TR-CD3 clustering on the cell surface and intracellular activation of LCK that phosphorylates the ITAM motifs of CD3G, CD3D, CD3E and CD247 enabling the recruitment of ZAP70. In turn ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NF-kappa-B (NF-kB) pathways, leading to the mobilization of transcription factors that are critical for gene expression and essential for T cell growth and differentiation (PubMed : 23524462). The T cell repertoire is generated in the thymus, by V-(D)-J rearrangement. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MH restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TR with the pMH complexes shapes TR structural and functional avidity (PubMed : 15040585).

See full target information TRBV7-9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell 41:1207-1221.e12 PubMed37327789

2023

The CD58-CD2 axis is co-regulated with PD-L1 via CMTM6 and shapes anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Ho,Johannes C Melms,Meri Rogava,Chris J Frangieh,Joanna Poźniak,Shivem B Shah,Zachary Walsh,Oleksandr Kyrysyuk,Amit Dipak Amin,Lindsay Caprio,Benjamin T Fullerton,Rajesh Kumar Soni,Casey R Ager,Jana Biermann,Yiping Wang,Mohsen Khosravi-Maharlooei,Giorgia Zanetti,Michael Mu,Hijab Fatima,Emily K Moore,Neil Vasan,Samuel F Bakhoum,Steven L Reiner,Chantale Bernatchez,Megan Sykes,Emily M Mace,Kai W Wucherpfennig,Dirk Schadendorf,Oliver Bechter,Parin Shah,Gary K Schwartz,Jean-Christophe Marine,Benjamin Izar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCR beta F1 antibody [8A3]

Flow Cytometry - Anti-TCR beta F1 antibody [8A3] (AB171088)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise