Anti-TCR delta antibody [EPR27043-16]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR delta antibody [EPR27043-16] (AB313573)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling TCR delta with ab313573 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Positive staining on human tonsil. The primary antibody was incubated for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TCR delta antibody [EPR27043-16] (AB313573)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human spleen tissue staining TCR delta with ab313573 at a 1/500 dilution, ab16669 anti-CD3 used at 1/100 dilution and ab227688 anti-CD19 used at a 1/100 dilution.

Panel A : merged staining of anti-TCR delta (green; Opal^™520), anti-CD3 (magenta; Opal^™570) and anti-CD19 (yellow; Opal^™690) on human colon.
Panel B : anti-TCR delta staining immune cells in human colon.
Panel C : anti-CD3 staining T lymphocytes in human colon.
Panel D : anti-CD19 staining B lymphocytes in human colon.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab313573, ab16669 and ab227688 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins..

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TCR delta antibody [EPR27043-16] (AB313573)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling TCR delta with ab313573 at 1/1000 dilution, followed by a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Negative control : no staining on human skeletal muscle. The primary antibody was incubated for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCR delta antibody [EPR27043-16] (AB313573)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling TCR delta with ab313573 at 1/100 (5.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cells transfected with a human TCR delta expression vector containing a myc tag. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 0.38ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCR delta antibody [EPR27043-16] (AB313573)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized human PBMC (human peripheral blood mononuclear cell) cells labelling TCR delta with ab313573 at 1/100 (5.08 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in subsets of CD3MSD278 human PBMCs. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-human CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-TCR delta antibody [EPR27043-16] (AB313573)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TCR delta with ab313573 at 1/50 dilution (1 µg)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD3 conjugated to Alexa Fluor® 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or primary antibody.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-TCR delta antibody [EPR27043-16] (AB313573)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling TCR delta with ab313573 at 1/50 dilution (1 µg)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were co-stained with anti-CD4 conjugated to APC-Fire™ 750. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or primary antibody.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-TCR delta antibody [EPR27043-16] (AB313573)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype / 293T (human embryonic kidney epithelial cell) transfected with a TRDC (human TCR delta) protein expression vector containing a myc-His tag / 293T transfected with a TRGC1 protein (human TCR gamma) expression vector / 293T transfected with an empty vector containing a myc-His tag cells labelling TCR delta with ab313573 at 1/50 dilution (1 ug)/Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TCR delta antibody [EPR27043-16] (AB313573)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human spleen tissue staining TCR delta with ab313573 at a 1/500 dilution, ab16669 anti-CD3 used at 1/100 dilution and ab227688 anti-CD19 used at a 1/100 dilution.

Panel A : merged staining of anti-TCR delta (green; Opal^™520), anti-CD3 (magenta; Opal^™570) and anti-CD19 (yellow; Opal^™690) on human spleen.
Panel B : anti-TCR delta staining immune cells in human spleen.
Panel C : anti-CD3 staining T lymphocytes in human spleen.
Panel D : anti-CD19 staining B lymphocytes in human spleen.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab313573, ab16669 and ab227688 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.