Mouse Monoclonal TCR gamma antibody. Carrier free. Suitable for Flow Cyt, FuncS and reacts with Human samples.
pH: 7.2
| Flow Cyt | FuncS | |
|---|---|---|
| Human | Expected | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes in 100ul volume. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
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Constant region of T cell receptor (TR) gamma chain that participates in the antigen recognition (PubMed:24600447). Gamma-delta TRs recognize a variety of self and foreign non-peptide antigens frequently expressed at the epithelial boundaries between the host and external environment, including endogenous lipids presented by MH-like protein CD1D and phosphoantigens presented by butyrophilin-like molecule BTN3A1. Upon antigen recognition induces rapid, innate-like immune responses involved in pathogen clearance and tissue repair (PubMed:23348415, PubMed:28920588). Binding of gamma-delta TR complex to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in the CD3 chains by the LCK and FYN kinases, allowing the recruitment, phosphorylation, and activation of ZAP70 that facilitates phosphorylation of the scaffolding proteins LCP2 and LAT. This lead to the formation of a supramolecular signalosome that recruits the phospholipase PLCG1, resulting in calcium mobilization and ERK activation, ultimately leading to T cell expansion and differentiation into effector cells (PubMed:25674089). Gamma-delta TRs are produced through somatic rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes. The potential diversity of gamma-delta TRs is conferred by the unique ability to rearrange (D) genes in tandem and to utilize all three reading frames. The combinatorial diversity is considerably increased by the sequence exonuclease trimming and random nucleotide (N) region additions which occur during the V-(D)-J rearrangements (PubMed:24387714).
TRDC
TCRGC2, TRGC2, T cell receptor gamma constant 2
Mouse Monoclonal TCR gamma antibody. Carrier free. Suitable for Flow Cyt, FuncS and reacts with Human samples.
pH: 7.2
Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by the LAL assay.
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The TCR gamma + TCR delta also known as gamma delta T-cell receptor is a part of the T-cell receptor (TCR) family. It has alternative names like gammadelta TCR or simply TCRδ/γ. The gamma and delta chains form a heterodimer that is part of the cell surface receptor complex. This complex is important for ligand recognition and immune response. The mass of the TCR gamma and TCR delta chains is not specified but they form between ZAP-70 and the CD3 complex on the surface of a subset of T-cells. These receptors are mainly expressed on gamma delta T-cells which are a minor population of T-cells in peripheral blood but more abundant in mucosal tissues and epithelial layers.
TCR gamma and TCR delta are involved in recognizing antigens without presentation by major histocompatibility complex (MHC) molecules. The receptor complex allows these cells to respond to a diverse range of non-peptide antigens mostly derived from microbial origin including phosphoantigens and lipids. This enables gamma delta T-cells to bridge innate and adaptive immunity. They do not always require antigen processing and can recognize antigens directly contributing efficiently to immune surveillance and early response to infections and cellular stress.
TCR gamma and TCR delta are important in the signaling networks that mediate immune responses. They are involved in pathways such as the nuclear factor of activated T-cells (NFAT) and mitogen-activated protein kinase (MAPK) pathways. These pathways promote the activation proliferation and differentiation of T-cells upon antigen recognition. The TCR complex including CD3 plays a role in these signal transduction events and ZAP-70 a protein tyrosine kinase is key for initiating these pathways upon TCR activation.
Mutations or dysregulation in TCR gamma and TCR delta can lead to immune-related conditions. For example abnormalities in these receptors have been linked to autoimmune diseases such as celiac disease and multiple sclerosis. Their role in these disorders is related to faulty recognition and response to autoantigens leading to inappropriate immune responses. Additionally gamma delta T-cells and their receptors are of interest in cancer research as they participate in tumor immunosurveillance. Connections with molecules like CD3 and ZAP-70 facilitate ongoing exploration for therapeutic interventions targeting immune-related pathologies.
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