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AB171103

Anti-TCR V delta 2 antibody [15D]

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(1 Publication)

Mouse Monoclonal TRDC antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human TRDC.

View Alternative Names

T cell receptor delta constant, TRDC

2 Images
Western blot - Anti-TCR V delta 2 antibody [15D] (AB171103)
  • WB

Supplier Data

Western blot - Anti-TCR V delta 2 antibody [15D] (AB171103)

Western blot analysis of TCR V delta 2 was performed by loading samples onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with primary antibody (ab171103) overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate.

All lanes:

Western blot - Anti-TCR V delta 2 antibody [15D] (ab171103) at 1/10 dilution

Lane 1:

Jurkat cell lysate at 25 µg

Lane 2:

CEM cell lysate at 25 µg

Observed band size: 19 kDa

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Flow Cytometry - Anti-TCR V delta 2 antibody [15D] (AB171103)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-TCR V delta 2 antibody [15D] (AB171103)

Flow cytometry analysis of TCR V delta 2 on M5 T cells. Equal numbers of cells were stained with a TCR V delta 2 Antibody (15D) monoclonal antibody (ab171103) followed by FITC labeled secondary antibody, or FITC labeled secondary antibody alone. 5 μL of primary antibody were used per test. All antibody incubations were performed for 30 minutes at room temperature. A representative 10,000 cells were acquired for each sample.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

15D

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Full Length Protein corresponding to Human TRDC. The exact immunogen used to generate this antibody is proprietary information.

B7Z8K6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µL for 10^4 Cells", "FlowCyt-species-notes": "<p>1-2 μg/test.</p><p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/100", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCR V delta 2 also known as Vγ9/Vδ2 T-cell receptor constitutes a specific chain of the gamma delta T-cell receptor present on the surface of some T cells. Weighing approximately 60 kDa TCR V delta 2 gets expressed in a subset of gamma delta T cells predominantly in peripheral blood of adult humans. Unlike alpha beta T-cell receptors this receptor recognizes antigens in a non-MHC-restricted manner making it unique in its mechanistic role in immune surveillance and response.
Biological function summary

TCR V delta 2 allows gamma delta T cells to identify and respond swiftly to peptide antigens typically associated with microbial infections. These cells do not require antigen processing or presentation by major histocompatibility complex (MHC) molecules and can respond directly to phosphoantigens presented by infected or transformed cells. In doing so they bridge innate and adaptive immunity by activating other immune cells like dendritic cells through cytokine release thereby modulating the immune response effectively.

Pathways

This T-cell receptor plays a notable role in the immune response and inflammation pathways. Key proteins involved in these pathways include CD3 a coreceptor that gets associated with TCR signaling amplifying the activation and function of gamma delta T cells. The receptor-mediated recognition and response to non-peptidic antigens activate downstream signaling pathways critical for cell-mediated cytotoxicity and cytokine production therefore advancing immune responses against pathogens or tumors.

TCR V delta 2 has associations with infections and cancer. Its ability to recognize and eliminate infected or malignant cells without the need for MHC molecules suggests a potential therapeutic role in controlling infections such as tuberculosis or combating various cancers. It connects closely with proteins like interleukin-2 which enhances the proliferation and activation of TCR V delta 2-bearing cells further contributing to its potential use in immunotherapy strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of T cell receptor (TR) delta chain that participates in the antigen recognition (PubMed : 24600447). Gamma-delta TRs recognize a variety of self and foreign non-peptide antigens frequently expressed at the epithelial boundaries between the host and external environment, including endogenous lipids presented by MH-like protein CD1D and phosphoantigens presented by butyrophilin-like molecule BTN3A1. Upon antigen recognition induces rapid, innate-like immune responses involved in pathogen clearance and tissue repair (PubMed : 23348415, PubMed : 28920588). Binding of gamma-delta TR complex to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in the CD3 chains by the LCK and FYN kinases, allowing the recruitment, phosphorylation, and activation of ZAP70 that facilitates phosphorylation of the scaffolding proteins LCP2 and LAT. This lead to the formation of a supramolecular signalosome that recruits the phospholipase PLCG1, resulting in calcium mobilization and ERK activation, ultimately leading to T cell expansion and differentiation into effector cells (PubMed : 25674089). Gamma-delta TRs are produced through somatic rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes. The potential diversity of gamma-delta TRs is conferred by the unique ability to rearrange (D) genes in tandem and to utilize all three reading frames. The combinatorial diversity is considerably increased by the sequence exonuclease trimming and random nucleotide (N) region additions which occur during the V-(D)-J rearrangements (PubMed : 24387714).
See full target information TRDC
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 5:14432 PubMed26395317

2015

Hyperactivation and in situ recruitment of inflammatory Vδ2 T cells contributes to disease pathogenesis in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Yin,Yujia Mao,Xuemei Li,Cai Yue,Chen Zhou,Linfang Huang,Wenxiu Mo,Di Liang,Jianmin Zhang,Wei He,Xuan Zhang
View all publications
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