AB171109

Anti-TCR V gamma 9 antibody [7A5]

Name: Anti-TCR V gamma 9 antibody [7A5] (ab171109) | Abcam
Brand: Abcam Limited
SKU: AB171109
Price: 510 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal TCR V gamma 9 antibody. Suitable for IP, Flow Cyt, ELISA, WB, Functional Study, IHC-Fr and reacts with Drosophila melanogaster, Primates, Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human TRGV9.

View Alternative Names

TCRGV9, TRGV9, T cell receptor gamma variable 9

2 Images

Flow Cytometry - Anti-TCR V gamma 9 antibody [7A5] (AB171109)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-TCR V gamma 9 antibody [7A5] (AB171109)

Flow cytometry analysis of TCR V gamma 9 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a TCR V gamma 9 monoclonal antibody (ab171109) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis

Supplier Data

Western blot - Anti-TCR V gamma 9 antibody [7A5] (AB171109)

Western blot analysis of TCR V gamma 9 was performed by loading 25 ug of CEM cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a TCR V gamma 9 monoclonal antibody (ab171109) at a dilution of 1 : 10 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using an ECL Plus Western Blotting Substrate.Results show a band at ~18-28 kDa.

All lanes:

Western blot - Anti-TCR V gamma 9 antibody [7A5] (ab171109)

true

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7A5

Isotype

IgG1

Carrier free

Reacts with

Human, Drosophila melanogaster, Primates

Applications

Flow Cyt, ELISA, IP, Functional Study, WB, IHC-Fr

applications

Immunogen

Full Length Protein corresponding to Human TRGV9. The exact immunogen used to generate this antibody is proprietary information.

Q99603

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FunctionalStudy" : {"fullname" : "Functional Study", "shortname":"Functional Study"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "2 μg/Test.<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1 - 1/100", "WB-species-notes": "", "FunctionalStudy-species-checked": "guaranteed", "FunctionalStudy-species-dilution-info": "", "FunctionalStudy-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Drosophila melanogaster": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "2 μg/Test.<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1 - 1/100", "WB-species-notes": "", "FunctionalStudy-species-checked": "guaranteed", "FunctionalStudy-species-dilution-info": "", "FunctionalStudy-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Primates": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "2 μg/Test.<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1 - 1/100", "WB-species-notes": "", "FunctionalStudy-species-checked": "guaranteed", "FunctionalStudy-species-dilution-info": "", "FunctionalStudy-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCR V gamma 9 also known as T cell receptor gamma 9 plays an important role in the immune system by recognizing antigens presented by non-classical MHC molecules. Measuring approximately 60 kDa TCR V gamma 9 forms part of the heterodimeric T cell receptor complex. It is particularly expressed on a subset of gamma delta T cells known as V gamma 9V delta 2 T cells which circulate in the blood and are involved in immune surveillance.

Biological function summary

TCR gamma 9 is involved in immune response to phosphoantigens derived from microbes and host metabolism. This receptor is part of a complex that includes the CD3 receptor protein which is required for signal transduction and T cell activation. The unique ability of V gamma 9V delta 2 T cells to recognize non-peptide antigens makes them different from the more common alpha beta T cells and positions them as important mediators in recognizing pathogen-induced stress.

Pathways

TCR V gamma 9 engages in immune cell signaling pathways such as the T-cell receptor signaling pathway and the MAPK signaling pathway. In these pathways it interacts with proteins like CD3 and ZAP-70 to facilitate T cell activation and proliferation. These interactions help initiate an immune response leading to the destruction of infected or cancerous cells.

TCR V gamma 9 has a role in infectious diseases like tuberculosis and certain cancers such as lymphoma. This receptor's interaction with CD3 plays a part in the host defense against Mycobacterium tuberculosis by recognizing unique phosphoantigens presented by this pathogen. Additionally alterations in gamma delta T cell function where TCR V gamma 9 is a core component correlate with cancer progression pointing to potential therapeutic interventions targeting this pathway in oncology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

V region of the variable domain of T cell receptor (TR) gamma chain that participates in the antigen recognition (PubMed : 24600447). Gamma-delta TRs recognize a variety of self and foreign non-peptide antigens frequently expressed at the epithelial boundaries between the host and external environment, including endogenous lipids presented by MH-like protein CD1D and phosphoantigens presented by butyrophilin-like molecule BTN3A1. Upon antigen recognition induces rapid, innate-like immune responses involved in pathogen clearance and tissue repair (PubMed : 23348415, PubMed : 28920588). Binding of gamma-delta TR complex to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in the CD3 chains by the LCK and FYN kinases, allowing the recruitment, phosphorylation, and activation of ZAP70 that facilitates phosphorylation of the scaffolding proteins LCP2 and LAT. This lead to the formation of a supramolecular signalosome that recruits the phospholipase PLCG1, resulting in calcium mobilization and ERK activation, ultimately leading to T cell expansion and differentiation into effector cells (PubMed : 25674089). Gamma-delta TRs are produced through somatic rearrangement of a limited repertoire of variable (V), diversity (D), and joining (J) genes. The potential diversity of gamma-delta TRs is conferred by the unique ability to rearrange (D) genes in tandem and to utilize all three reading frames. The combinatorial diversity is considerably increased by the sequence exonuclease trimming and random nucleotide (N) region additions which occur during the V-(D)-J rearrangements (PubMed : 24387714).

See full target information TRGV9

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 5:14432 PubMed26395317

2015

Hyperactivation and in situ recruitment of inflammatory Vδ2 T cells contributes to disease pathogenesis in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Shanshan Yin,Yujia Mao,Xuemei Li,Cai Yue,Chen Zhou,Linfang Huang,Wenxiu Mo,Di Liang,Jianmin Zhang,Wei He,Xuan Zhang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TCR V gamma 9 antibody [7A5]

Flow Cytometry - Anti-TCR V gamma 9 antibody [7A5] (AB171109)

Western blot - Anti-TCR V gamma 9 antibody [7A5] (AB171109)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise