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AB133568

Anti-TCTP antibody [EPR5540]

5

(3 Reviews)

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(11 Publications)

Rabbit Recombinant Monoclonal TCTP antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

View Alternative Names

Translationally-controlled tumor protein, TCTP, Fortilin, Histamine-releasing factor, p23, HRF, TPT1

3 Images
Flow Cytometry (Intracellular) - Anti-TCTP antibody [EPR5540] (AB133568)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TCTP antibody [EPR5540] (AB133568)

Overlay histogram showing A431 cells stained with ab133568 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133568, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (AB133568)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCTP antibody [EPR5540] (AB133568)

Immunofluorescence analysis of A431 cells staining TCTP (green) using ab133568 at a 1/250 dilution. Cell nuclei are shown in blue.

Western blot - Anti-TCTP antibody [EPR5540] (AB133568)
  • WB

Unknown

Western blot - Anti-TCTP antibody [EPR5540] (AB133568)

All lanes:

Western blot - Anti-TCTP antibody [EPR5540] (ab133568) at 1/10000 dilution

Lane 1:

Raji cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 20 kDa

true

  • Carrier free

    Anti-TCTP antibody [EPR5540] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5540

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCTP also known as Translationally Controlled Tumor Protein or histamine-releasing factor (HRF) is a multifunctional protein with a molecular mass of approximately 19 kDa. It is found in a variety of tissues and cell types including cytoplasm and extracellular environments. The protein engages in cellular processes such as cell growth development and apoptosis. TCTP regulates microtubule stability cell cycle progression and protein synthesis reflecting its broad mechanical roles within cells.
Biological function summary

In both animal and human cells TCTP acts as a prosurvival factor by inhibiting apoptosis. It binds with proteins like Bcl-2 and Bax playing a role in the regulation of cell death. Furthermore TCTP appears to mediate immune responses through its histamine-releasing factor activity. It interacts within protein complexes that manage cytoskeletal dynamics and stress responses contributing to cellular adaptability.

Pathways

The protein interacts in critical pathways such as the MAPK signaling pathway and the apoptosis pathway. In the MAPK pathway TCTP modulates cell growth and proliferation by interacting with proteins like ERK1/2. In the apoptosis pathway TCTP's interaction with Bcl-xL and Bax highlights its role in the fine-tuning of cell survival and death processes. These pathways highlight its participation in essential cellular functions and disease progression.

TCTP has implications in cancer and allergy. Altered expression and function of TCTP have been linked to tumor growth and resistance to cancer therapies often regulated through complex interactions with proteins like p53. In allergies its role in histamine release positions it as a mediator in allergic asthma and other hypersensitivity reactions. These associations emphasize the clinical relevance of TCTP and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in calcium binding and microtubule stabilization (PubMed : 12167714, PubMed : 15162379, PubMed : 15958728). Acts as a negative regulator of TSC22D1-mediated apoptosis, via interaction with and destabilization of TSC22D1 protein (PubMed : 18325344).
See full target information Translationally-controlled tumor protein
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1 PubMed39747445

2025

Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mari Nakashima,Decha Pinkaew,Uttariya Pal,Fei Miyao,Hanna Huynh,Lena Tanaka,Ken Fujise

Antioxidants (Basel, Switzerland) 12: PubMed37107185

2023

Deubiquitylase OTUD3 Mediates Endoplasmic Reticulum Stress through Regulating Fortilin Stability to Restrain Dopaminergic Neurons Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Chen,Xuejie Huan,Fengju Jia,Zhen Zhang,Mingxia Bi,Lin Fu,Xixun Du,Xi Chen,Chunling Yan,Qian Jiao,Hong Jiang

Communications biology 5:157 PubMed35197550

2022

Fortilin interacts with TGF-β1 and prevents TGF-β receptor activation.

Applications

Unspecified application

Species

Unspecified reactive species

Decha Pinkaew,Erik Martinez-Hackert,Wei Jia,Matthew D King,Fei Miao,Nicole R Enger,Runglawan Silakit,Kota Ramana,Shi-You Chen,Ken Fujise

Frontiers in molecular biosciences 8:686718 PubMed34589516

2021

Upregulation of Translationally Controlled Tumor Protein Is Associated With Cervical Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyu Zhu,Ji Ren,Dianqin Xu,Di Cheng,Wei Wang,Jie Ren,Ziwen Xiao,Hongmei Jiang,Yan Ding,Yujie Tan

Cells 9: PubMed32438775

2020

DHA Affects Microtubule Dynamics Through Reduction of Phospho-TCTP Levels and Enhances the Antiproliferative Effect of T-DM1 in Trastuzumab-Resistant HER2-Positive Breast Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia D'Amico,Ewa Krystyna Krasnowska,Isabella Manni,Gabriele Toietta,Silvia Baldari,Giulia Piaggio,Marco Ranalli,Alessandra Gambacurta,Claudio Vernieri,Flavio Di Giacinto,Francesca Bernassola,Filippo de Braud,Maria Lucibello

OncoTargets and therapy 12:1641-1653 PubMed30881019

2019

TCTP promotes epithelial-mesenchymal transition in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ruilin Sun,Xi Lu,Li Gong,Faguang Jin

Clinical and translational radiation oncology 15:99-107 PubMed30815593

2019

Assessment of the role of translationally controlled tumor protein 1 (TPT1/TCTP) in breast cancer susceptibility and ATM signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Katharina Neuhäuser,Leonie Küper,Hans Christiansen,Natalia Bogdanova

International journal of clinical and experimental 11:2522-2529 PubMed31938365

2018

miR-455-3p functions as a tumor suppressor in colorectal cancer and inhibits cell proliferation by targeting TPT1.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Sun,Yan Wang,Hainan Yang,Yan Xu,Haipeng Yu

Nature communications 8:18 PubMed28550308

2017

Fortilin binds IRE1α and prevents ER stress from signaling apoptotic cell death.

Applications

WB

Species

Unspecified reactive species

Decha Pinkaew,Abhijnan Chattopadhyay,Matthew D King,Preedakorn Chunhacha,Zhihe Liu,Heather L Stevenson,Yanjie Chen,Patuma Sinthujaroen,Owen M McDougal,Ken Fujise

PloS one 10:e0116270 PubMed25643152

2015

Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ∆133p53α mimic.

Applications

WB

Species

Mouse

Sonal Sawhney,Kylie Hood,Alisha Shaw,Antony W Braithwaite,Richard Stubbs,Noelyn A Hung,Janice A Royds,Tania L Slatter
View all publications
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Product promise

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