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AB326110

Anti-TCTP/TPT1 antibody [EPR5541] - BSA and Azide free

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Rabbit Recombinant Monoclonal TCTP antibody. Carrier free. Suitable for WB, Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse, Rat samples.

View Alternative Names

Translationally-controlled tumor protein, TCTP, Fortilin, Histamine-releasing factor, p23, HRF, TPT1

3 Images
Flow Cytometry (Intracellular) - Anti-TCTP/TPT1 antibody [EPR5541] - BSA and Azide free (AB326110)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TCTP/TPT1 antibody [EPR5541] - BSA and Azide free (AB326110)

This data was developed using ab124835, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Jurkat cells labelling TCTP with ab124835 at 1/20 dilution (7.5 µg) (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488 ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-TCTP/TPT1 antibody [EPR5541] - BSA and Azide free (AB326110)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TCTP/TPT1 antibody [EPR5541] - BSA and Azide free (AB326110)

This data was developed using ab124835, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized Jurkat cells with ab124835 at 1/50 (3.08 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in Jurkat cells. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Western blot - Anti-TCTP/TPT1 antibody [EPR5541] - BSA and Azide free (AB326110)
  • WB

Supplier Data

Western blot - Anti-TCTP/TPT1 antibody [EPR5541] - BSA and Azide free (AB326110)

This data was developed using ab124835, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TCTP antibody [EP5541] (<a href='/en-us/products/primary-antibodies/tctp-antibody-ep5541-ab124835'>ab124835</a>) at 1/10000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 15 µg

Lanes 2 - 3:

Mouse brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 22 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5541

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab326110 is the carrier-free version of ab124835

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TCTP also known as Translationally Controlled Tumor Protein or histamine-releasing factor (HRF) is a multifunctional protein with a molecular mass of approximately 19 kDa. It is found in a variety of tissues and cell types including cytoplasm and extracellular environments. The protein engages in cellular processes such as cell growth development and apoptosis. TCTP regulates microtubule stability cell cycle progression and protein synthesis reflecting its broad mechanical roles within cells.
Biological function summary

In both animal and human cells TCTP acts as a prosurvival factor by inhibiting apoptosis. It binds with proteins like Bcl-2 and Bax playing a role in the regulation of cell death. Furthermore TCTP appears to mediate immune responses through its histamine-releasing factor activity. It interacts within protein complexes that manage cytoskeletal dynamics and stress responses contributing to cellular adaptability.

Pathways

The protein interacts in critical pathways such as the MAPK signaling pathway and the apoptosis pathway. In the MAPK pathway TCTP modulates cell growth and proliferation by interacting with proteins like ERK1/2. In the apoptosis pathway TCTP's interaction with Bcl-xL and Bax highlights its role in the fine-tuning of cell survival and death processes. These pathways highlight its participation in essential cellular functions and disease progression.

TCTP has implications in cancer and allergy. Altered expression and function of TCTP have been linked to tumor growth and resistance to cancer therapies often regulated through complex interactions with proteins like p53. In allergies its role in histamine release positions it as a mediator in allergic asthma and other hypersensitivity reactions. These associations emphasize the clinical relevance of TCTP and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in calcium binding and microtubule stabilization (PubMed : 12167714, PubMed : 15162379, PubMed : 15958728). Acts as a negative regulator of TSC22D1-mediated apoptosis, via interaction with and destabilization of TSC22D1 protein (PubMed : 18325344).
See full target information Translationally-controlled tumor protein
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com