AB245620

Anti-TDG antibody

Name: Anti-TDG antibody (ab245620) | Abcam
Brand: Abcam Limited
SKU: AB245620

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(1 Publication)

Rabbit Polyclonal TDG antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TDG aa 350 to C-terminus.

View Alternative Names

G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase, hTDG, TDG

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDG antibody (AB245620)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDG antibody (AB245620)

Formalin-fixed, paraffin-embedded human mammary gland tissue stained for TDG using ab245620 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDG antibody (AB245620)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for TDG using ab245620 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Immunoprecipitation - Anti-TDG antibody (AB245620)

TDG was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) with ab245620 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using ab245620 at 1 μg/ml.

Lane 1 : ab245620 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG IP in HEK-293T whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-TDG antibody (ab245620)

Predicted band size: 46 kDa

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Supplier Data

Western blot - Anti-TDG antibody (AB245620)

All lanes:

Western blot - Anti-TDG antibody (ab245620) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 46 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human TDG aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13569

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab245620 was affinity purified using an epitope specific to TDG immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDG also known as thymine DNA glycosylase is an enzyme that plays a critical role in DNA repair. It has a molecular mass of approximately 45 kDa. TDG removes thymine and other modified bases from DNA that are paired with guanine which initiates the process of base excision repair. This mechanism is essential for maintaining genomic integrity. TDG is expressed in various tissues including the brain liver and testes reflecting its importance in diverse physiological contexts.

Biological function summary

Thymine DNA glycosylase is involved in the active DNA demethylation process. It participates in the DNA repair machinery as part of the base excision repair complex interacting with other proteins involved in this pathway. TDG's function guarantees the correct removal and replacement of erroneous bases which might otherwise result in mutations or faulty gene expression. This activity is tightly regulated to ensure accurate DNA maintenance and cellular function.

Pathways

TDG functions within the base excision repair and DNA demethylation pathways. In these pathways proteins such as APE1 (apurinic/apyrimidinic endodeoxyribonuclease 1) work closely with TDG to process intermediates generated during base removal. Through base excision repair TDG contributes to the protection against mutations by correcting base mispairings. The DNA demethylation pathway in which TDG participates also plays a role in the epigenetic regulation of gene expression influencing developmental processes and cellular differentiation.

TDG's function has implications for cancer and neurodegenerative diseases. Altered TDG activity can lead to genomic instability contributing to the progression of cancer by allowing the accumulation of mutations. Furthermore disruptions in DNA repair mechanisms including those involving TDG have been linked to neurodegenerative diseases such as Alzheimer's disease. In cancer proteins like MSH6 in the mismatch repair pathway might interact with TDG when aberrant repair occurs. In neurodegenerative disorders changes in TDG activity might interact with proteins involved in neuronal function and stability exacerbating disease mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA glycosylase that plays a key role in active DNA demethylation : specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs : in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

See full target information TDG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Toxicology research 13:tfae096 PubMed38957783

2024

LncRNA HILPDA promotes contrast-induced acute kidney injury by recruiting eIF4B to upregulate XPO1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyan Wei,Tuersun Tilakezi,Wei Feng,Heyin Yang,Shujun Yang

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TDG antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDG antibody (AB245620)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDG antibody (AB245620)

Immunoprecipitation - Anti-TDG antibody (AB245620)

Western blot - Anti-TDG antibody (AB245620)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise