Rabbit Recombinant Monoclonal TDG antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (AB249089), expandable thumbnail](https://content.abcam.com/products/images/tdg-antibody-epr8774-bsa-and-azide-free-ab249089--western-blot-img28322.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (AB249089)")
![Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (AB249089), expandable thumbnail](https://content.abcam.com/products/images/tdg-antibody-epr8774-bsa-and-azide-free-ab249089--western-blot-img102592.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (AB249089)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Expected |
| Mouse | Not recommended | Predicted | Not recommended | Not recommended | Expected |
| Rat | Not recommended | Predicted | Not recommended | Not recommended | Expected |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt (Intra)
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.
Alternative names
G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase, hTDG, TDG
Recommended products
Rabbit Recombinant Monoclonal TDG antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8774
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab249089 is the carrier-free version of Anti-TDG antibody [EPR8774] ab154192.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TDG also known as thymine DNA glycosylase is an enzyme that plays a critical role in DNA repair. It has a molecular mass of approximately 45 kDa. TDG removes thymine and other modified bases from DNA that are paired with guanine which initiates the process of base excision repair. This mechanism is essential for maintaining genomic integrity. TDG is expressed in various tissues including the brain liver and testes reflecting its importance in diverse physiological contexts.
- Biological function summary
Thymine DNA glycosylase is involved in the active DNA demethylation process. It participates in the DNA repair machinery as part of the base excision repair complex interacting with other proteins involved in this pathway. TDG's function guarantees the correct removal and replacement of erroneous bases which might otherwise result in mutations or faulty gene expression. This activity is tightly regulated to ensure accurate DNA maintenance and cellular function.
- Pathways
TDG functions within the base excision repair and DNA demethylation pathways. In these pathways proteins such as APE1 (apurinic/apyrimidinic endodeoxyribonuclease 1) work closely with TDG to process intermediates generated during base removal. Through base excision repair TDG contributes to the protection against mutations by correcting base mispairings. The DNA demethylation pathway in which TDG participates also plays a role in the epigenetic regulation of gene expression influencing developmental processes and cellular differentiation.
- Associated diseases and disorders
TDG's function has implications for cancer and neurodegenerative diseases. Altered TDG activity can lead to genomic instability contributing to the progression of cancer by allowing the accumulation of mutations. Furthermore disruptions in DNA repair mechanisms including those involving TDG have been linked to neurodegenerative diseases such as Alzheimer's disease. In cancer proteins like MSH6 in the mismatch repair pathway might interact with TDG when aberrant repair occurs. In neurodegenerative disorders changes in TDG activity might interact with proteins involved in neuronal function and stability exacerbating disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (ab249089), expandable thumbnail](https://content.abcam.com/products/images/tdg-antibody-epr8774-bsa-and-azide-free-ab249089--western-blot-img28322.jpg "Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (ab249089)")
Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (ab249089)
This data was developed using Anti-TDG antibody [EPR8774] ab154192, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TDG antibody [EPR8774] (Anti-TDG antibody [EPR8774] ab154192) at 1/5000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Mouse spleen tissue lysate at 20 µg
Lane 5: Rat brain tissue lysate at 20 µg
Lane 6: Rat heart tissue lysate at 20 µg
Lane 7: Rat kidney tissue lysate at 20 µg
Lane 8: Rat spleen tissue lysate at 20 µg
Predicted band size: 46 kDa
Observed band size: 55-60 kDa
Exposure time: 3min
![Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (ab249089), expandable thumbnail](https://content.abcam.com/products/images/tdg-antibody-epr8774-bsa-and-azide-free-ab249089--western-blot-img102592.jpg "Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (ab249089)")
Western blot - Anti-TDG antibody [EPR8774] - BSA and Azide free (ab249089)
This data was developed using Anti-TDG antibody [EPR8774] ab154192, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-TDG antibody [EPR8774] (Anti-TDG antibody [EPR8774] ab154192) at 1/1000 dilution
Lane 1: Fetal lung cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: K562 cell lysate at 10 µg
Lane 5: Fetal brain cell lysate at 10 µg
Predicted band size: 46 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com