AB76859

Anti-TDO2/TDO antibody

Name: Anti-TDO2/TDO antibody (ab76859) | Abcam
Brand: Abcam Limited
SKU: AB76859
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Mouse Polyclonal TDO2/TDO antibody. Carrier free. Suitable for ELISA, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TDO2.

View Alternative Names

TDO, TDO2, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase, TO, TRPO

1 Images

Unknown

Western blot - Anti-TDO2/TDO antibody (AB76859)

All lanes:

Western blot - Anti-TDO2/TDO antibody (ab76859) at 1/500 dilution

Lane 1:

TDO2/TDO transfected 293T cells lysate at 25 µg

Lane 2:

Non transfected 293T cells lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TDO2.

P48775

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDO2 also known as tryptophan 23-dioxygenase is an enzyme that catalyzes the initial and rate-limiting step in the kynurenine pathway degrading tryptophan to N-formylkynurenine. This reaction is important for regulating systemic tryptophan levels. TDO2 is a heme-containing enzyme with a molecular mass of approximately 53 kDa. It is highly expressed in the liver unlike its counterpart indoleamine 23-dioxygenase (IDO) which has wider tissue distribution. Liver-specific expression suggests its role in orchestrating whole-body tryptophan metabolism.

Biological function summary

TDO2 plays a significant role in maintaining immune tolerance and modulating neurotransmitter levels. While not part of a larger complex it interacts with several other proteins to mediate its effects. By controlling tryptophan availability TDO2 impacts the production of serotonin and downstream kynurenine metabolites affecting neural and immune functions. Its activity influences both central nervous system processes and peripheral immune responses implying a broad impact on various physiological systems.

Pathways

TDO2's function integrates into the kynurenine and serotonin pathways. In the kynurenine pathway TDO2's enzymatic activity leads to the generation of metabolites such as kynurenine and quinolinic acid which have immunoregulatory and excitotoxic properties. The serotonin pathway also indirectly involves TDO2 as it limits tryptophan's availability for serotonin synthesis. TDO2 interacts with IDO in controlling the balance of tryptophan metabolites providing a link between immune signaling and central nervous system regulation.

TDO2 has been implicated in cancer and neurodegenerative diseases. Overexpression of TDO2 is observed in various cancers where it helps create an immune-suppressive environment that aids tumor survival. The enzyme’s upregulation affects tumor microenvironment dynamics often acting in concert with IDO which further mediates immune escape. TDO2's activity may also contribute to neurodegenerative disorders through its impact on kynurenine pathway metabolites which can be neurotoxic. The interplay between TDO2 and IDO suggests that targeting this enzymatic pathway could offer therapeutic avenues in both oncological and neuropsychiatric conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety.

See full target information TDO2

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 12:2558-2575 PubMed35812057

2022

TDO2 and tryptophan metabolites promote kynurenine/AhR signals to facilitate glioma progression and immunosuppression.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanhong Zhong,Lilei Peng,Bei Tao,Senlin Yin,Liang Lyu,Hao Ding,Xiaobo Yang,Tangming Peng,Haiping He,Peizhi Zhou

The FEBS journal 288:4311-4331 PubMed33471408

2021

Pharmacological validation of TDO as a target for Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Perez-Pardo,Yvonne Grobben,Nicole Willemsen-Seegers,Mitch Hartog,Michaela Tutone,Michelle Muller,Youri Adolfs,Ronald Jeroen Pasterkamp,Diep Vu-Pham,Antoon M van Doornmalen,Freek van Cauter,Joeri de Wit,Jan Gerard Sterrenburg,Joost C M Uitdehaag,Jos de Man,Rogier C Buijsman,Guido J R Zaman,Aletta D Kraneveld

Molecular human reproduction 21:320-9 PubMed25542835

2014

Identification of a human subcortical maternal complex.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhu,Liying Yan,Xiaoxin Zhang,Xukun Lu,Tianren Wang,Jie Yan,Xinqi Liu,Jie Qiao,Lei Li

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TDO2/TDO antibody

Western blot - Anti-TDO2/TDO antibody (AB76859)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise