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Rabbit Recombinant Monoclonal TDO2/TDO antibody. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (AB259359), expandable thumbnail
  • Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (AB259359), expandable thumbnail
  • Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (AB259359), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TDO2/TDO antibody [EPR23745-311] (AB259359), expandable thumbnail
  • Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (AB259359), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PIHC-FrFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Expected
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Expected
Expected
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/500

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/30

Notes

-

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety.

Alternative names

TDO, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase, TO, TRPO, TDO2, TDO

Recommended products

Rabbit Recombinant Monoclonal TDO2/TDO antibody. Suitable for ICC/IF, IP, WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR23745-311

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TDO2 also known as tryptophan 23-dioxygenase is an enzyme that catalyzes the initial and rate-limiting step in the kynurenine pathway degrading tryptophan to N-formylkynurenine. This reaction is important for regulating systemic tryptophan levels. TDO2 is a heme-containing enzyme with a molecular mass of approximately 53 kDa. It is highly expressed in the liver unlike its counterpart indoleamine 23-dioxygenase (IDO) which has wider tissue distribution. Liver-specific expression suggests its role in orchestrating whole-body tryptophan metabolism.

Biological function summary

TDO2 plays a significant role in maintaining immune tolerance and modulating neurotransmitter levels. While not part of a larger complex it interacts with several other proteins to mediate its effects. By controlling tryptophan availability TDO2 impacts the production of serotonin and downstream kynurenine metabolites affecting neural and immune functions. Its activity influences both central nervous system processes and peripheral immune responses implying a broad impact on various physiological systems.

Pathways

TDO2's function integrates into the kynurenine and serotonin pathways. In the kynurenine pathway TDO2's enzymatic activity leads to the generation of metabolites such as kynurenine and quinolinic acid which have immunoregulatory and excitotoxic properties. The serotonin pathway also indirectly involves TDO2 as it limits tryptophan's availability for serotonin synthesis. TDO2 interacts with IDO in controlling the balance of tryptophan metabolites providing a link between immune signaling and central nervous system regulation.

Associated diseases and disorders

TDO2 has been implicated in cancer and neurodegenerative diseases. Overexpression of TDO2 is observed in various cancers where it helps create an immune-suppressive environment that aids tumor survival. The enzyme’s upregulation affects tumor microenvironment dynamics often acting in concert with IDO which further mediates immune escape. TDO2's activity may also contribute to neurodegenerative disorders through its impact on kynurenine pathway metabolites which can be neurotoxic. The interplay between TDO2 and IDO suggests that targeting this enzymatic pathway could offer therapeutic avenues in both oncological and neuropsychiatric conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359), expandable thumbnail

    Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The the expression pattern and observed MW is consistent with what has been described in the literature (PMID: 27573671, PMID: 25691885)

    Negative control: pancreas (PMID:25691885).

    Exposure time: 26 seconds

    All lanes: Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359) at 1/1000 dilution

    Lane 1: Human liver tissue lysate at 40 µg

    Lane 2: Human pancreas tissue lysate at 40 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 48 kDa

  • Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359), expandable thumbnail

    Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression pattern and observed MW is consistent with what has been described in the literature (PMID:25691885, PMID: 10775460).

    Negative control: pancreas (PMID:25691885).

    Exposure time: 26 seconds.

    All lanes: Western blot - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 40 µg

    Lane 2: Mouse pancreas tissue lysate at 40 µg

    Lane 3: Rat liver tissue lysate at 40 µg

    Lane 4: Rat pancreas tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa

    Observed band size: 43 kDa

    This data was developed using 259359, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression pattern and observed MW is consistent with what has been described in the literature (PMID:25691885, PMID: 10775460).

    Negative control: pancreas (PMID:25691885).

    Exposure time: 26 seconds.

  • Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359), expandable thumbnail

    Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

    TDO2/TDO was immunoprecipitated from 0.35 mg Human liver tissue lysate 10 ug with ab259359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human liver tissue lysate 10 ug

    Lane 2: ab259359 IP in Human liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259359 in human liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

    Predicted band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T cells labelling TDO2/TDO with ab259359 at 1/500 (1.072 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/500 dilution (Green) Confocal image showing cytoplasmic staining in 293T cells transfected with myc-tagged TDO2 expression vector. is observed. Myc-Tag Mouse mAb (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/500 dilution.

  • Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359), expandable thumbnail

    Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

    TDO2/TDO was immunoprecipitated from 0.35 mg Mouse liver tissue lysate 10 ug with ab259359 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259359 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver tissue lysate 10 ug

    Lane 2: ab259359 IP in Mouse liver tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259359 in mouse liver tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-TDO2/TDO antibody [EPR23745-311] (ab259359)

    Predicted band size: 48 kDa

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Product protocols

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