AB273654

Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal TDO2/TDO antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TDO2.

View Alternative Names

TDO, TDO2, Tryptophan oxygenase, Tryptophan pyrrolase, Tryptophanase, TO, TRPO

10 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

Immunohistochemical analysis of paraffin-embedded human colon tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

Immunohistochemical analysis of paraffin-embedded human breast tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

Immunohistochemical analysis of paraffin-embedded human endometrium adenocarcinoma tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

Immunohistochemical analysis of paraffin-embedded human prostate tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

Immunohistochemical analysis of paraffin-embedded human liver tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

Immunohistochemical analysis of paraffin-embedded human ovary adenocarcinoma tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue staining TDO2 with a mouse monoclonal TDO2/TDO antibody [OTI2A4].

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.

Western blot - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

WB

Supplier Data

Western blot - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (AB273654)

All lanes:

Western blot - Anti-TDO2/TDO antibody [OTI2A4] - BSA and Azide free (ab273654) at 1/2000 dilution

Lane 1:

pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Lane 2:

pCMV6-ENTRY TDO2-transfected HEK-293T whole cell lysate at 20 µg

Predicted band size: 48 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2A4

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

Immunogen

Recombinant Full Length Protein corresponding to Human TDO2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.3 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDO2 also known as tryptophan 23-dioxygenase is an enzyme that catalyzes the initial and rate-limiting step in the kynurenine pathway degrading tryptophan to N-formylkynurenine. This reaction is important for regulating systemic tryptophan levels. TDO2 is a heme-containing enzyme with a molecular mass of approximately 53 kDa. It is highly expressed in the liver unlike its counterpart indoleamine 23-dioxygenase (IDO) which has wider tissue distribution. Liver-specific expression suggests its role in orchestrating whole-body tryptophan metabolism.

Biological function summary

TDO2 plays a significant role in maintaining immune tolerance and modulating neurotransmitter levels. While not part of a larger complex it interacts with several other proteins to mediate its effects. By controlling tryptophan availability TDO2 impacts the production of serotonin and downstream kynurenine metabolites affecting neural and immune functions. Its activity influences both central nervous system processes and peripheral immune responses implying a broad impact on various physiological systems.

Pathways

TDO2's function integrates into the kynurenine and serotonin pathways. In the kynurenine pathway TDO2's enzymatic activity leads to the generation of metabolites such as kynurenine and quinolinic acid which have immunoregulatory and excitotoxic properties. The serotonin pathway also indirectly involves TDO2 as it limits tryptophan's availability for serotonin synthesis. TDO2 interacts with IDO in controlling the balance of tryptophan metabolites providing a link between immune signaling and central nervous system regulation.

TDO2 has been implicated in cancer and neurodegenerative diseases. Overexpression of TDO2 is observed in various cancers where it helps create an immune-suppressive environment that aids tumor survival. The enzyme’s upregulation affects tumor microenvironment dynamics often acting in concert with IDO which further mediates immune escape. TDO2's activity may also contribute to neurodegenerative disorders through its impact on kynurenine pathway metabolites which can be neurotoxic. The interplay between TDO2 and IDO suggests that targeting this enzymatic pathway could offer therapeutic avenues in both oncological and neuropsychiatric conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Heme-dependent dioxygenase that catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring and converts L-tryptophan to N-formyl-L-kynurenine. Catalyzes the oxidative cleavage of the indole moiety.

See full target information TDO2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell biology international 46:1577-1587 PubMed35702760

2022

Kynurenine produced by tryptophan 2,3-dioxygenase metabolism promotes glioma progression through an aryl hydrocarbon receptor-dependent signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weichao Ma,Lu Ye,Chuanhong Zhong,Jianguo Li,Feng Ye,Liang Lv,Yang Yu,Shu Jiang,Peizhi Zhou

View all publications

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