AB224822

Anti-TDP1 antibody

Name: Anti-TDP1 antibody (ab224822) | Abcam
Brand: Abcam Limited
SKU: AB224822
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal TDP1 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TDP1 aa 1-50.

View Alternative Names

Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, TDP1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP1 antibody (AB224822)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP1 antibody (AB224822)

Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for TDP1 using ab224822 at 1/1000 dilution in immunohistochemical analysis. Detection : DAB staining.

Supplier Data

Immunoprecipitation - Anti-TDP1 antibody (AB224822)

TDP1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab224822 at 3 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab224822 at 0.4 μg/ml.

Lane 1 : ab224822 IP in HeLa whole cell lysate.

Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TDP1 antibody (ab224822)

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-TDP1 antibody (AB224822)

All lanes:

Western blot - Anti-TDP1 antibody (ab224822) at 0.04 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg

Lane 4:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Predicted band size: 68 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB

applications

Immunogen

Synthetic Peptide within Human TDP1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9NUW8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab224822 was affinity purified using an epitope specific to TDP1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDP1 short for Tyrosyl-DNA Phosphodiesterase 1 is an enzyme involved in the repair of DNA damage. Also known as SCAN1 protein this enzyme has a mass of approximately 60 kDa and is expressed in a variety of tissues with higher levels seen in the brain and heart. It plays a mechanical role by hydrolyzing the phosphodiester bond between a DNA 3'-phosphate and a tyrosine residue an important step in resolving topoisomerase I-DNA crosslinks during the repair of stalled replication forks.

Biological function summary

TDP1 contributes significantly to the maintenance of genomic stability. It functions primarily as a DNA repair enzyme acting independently rather than as part of a protein complex. TDP1's ability to cleave phosphotyrosyl bonds makes it integral to processes that safeguard against DNA strand breaks induced by oxidative stress or other exogenous factors. Though not part of a complex its function is supported by the interaction with proteins like DNA polymerase which aids in the subsequent steps of DNA repair synthesis.

Pathways

TDP1 is involved in the DNA damage response and repair pathways. It collaborates heavily with the Base Excision Repair (BER) and the Single-Strand Break Repair (SSBR) pathways. TDP1 works alongside proteins like PARP1 facilitating the repair of single-strand DNA breaks which is critical for cell survival and prevention of mutations. Aphidicolin a known inhibitor of DNA polymerase can affect DNA synthesis steps that follow TDP1 activity indicating how interconnected these pathways are.

TDP1 has notable associations with certain neurodegenerative diseases and cancer. Mutations or dysregulation in TDP1 have been implicated in the autosomal recessive disorder known as Spinocerebellar Ataxia with Axonal Neuropathy (SCAN1). Additionally due to its role in DNA repair TDP1 may influence cancer progression as cells with impaired DNA repair capabilities accumulate mutations that could lead to tumorigenesis. The interaction with methotrexate a chemotherapeutic agent can also affect cancer treatment outcomes given methotrexate's influence on folate metabolism and DNA synthesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate.

See full target information TDP1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108247 PubMed39894218

2025

A BRAF-activated noncoding RNA attenuates clear cell renal cell carcinoma via repression of glucose-6-phosphate dehydrogenase.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Liu,Yueli Ni,Honggang Bai,Xiangjie Liu,Asif Shahzad,Kun Cui,Qiuxin Duan,Ziyuan Bai,Yurong Dong,Zihan Yi,Buqing Sai,Yingmin Kuang,Chen Guo,Yuechun Zhu,Qiao Zhang,Zhe Yang

Cell reports 43:114214 PubMed38761375

2024

TDP1 mutation causing SCAN1 neurodegenerative syndrome hampers the repair of transcriptional DNA double-strand breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Mathéa Geraud,Agnese Cristini,Simona Salimbeni,Nicolas Bery,Virginie Jouffret,Marco Russo,Andrea Carla Ajello,Lara Fernandez Martinez,Jessica Marinello,Pierre Cordelier,Didier Trouche,Gilles Favre,Estelle Nicolas,Giovanni Capranico,Olivier Sordet

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TDP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP1 antibody (AB224822)

Immunoprecipitation - Anti-TDP1 antibody (AB224822)

Western blot - Anti-TDP1 antibody (AB224822)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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