Anti-TDP1 antibody

• WB

Collaborator

Western blot - Anti-TDP1 antibody (AB4166)

ab4166 was incubated for 1 hr at room temp.

Lane 1:

Molecular weight markers

Lanes 2 - 5:

Western blot - Anti-TDP1 antibody (ab4166) at 1/1000 dilution

Lane 2:

1 ng purified recombinant human TDP1

Lane 3:

3 ng purified recombinant human TDP1

Lane 4:

6 ng purified recombinant human TDP1

Lane 5:

6 micrograms total protein from MCF7 human cell extract

Predicted band size: 68 kDa

Observed band size: 68 kDa

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This image is courtesy of Keith Caldecott, University of Sussex

• WB

Lab

Western blot - Anti-TDP1 antibody (AB4166)

All lanes:

Western blot - Anti-TDP1 antibody (ab4166) at 1 µg/mL

All lanes:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 68 kDa

Observed band size: 75 kDa

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Exposure time: 4min

• WB

CiteAb

Western blot - Anti-TDP1 antibody (AB4166)

TDP1 western blot using anti-TDP1 antibody ab4166. Publication image and figure legend from Walker, S., Meisenberg, C., et al., 2014, Anal Biochem, PubMed 24637157.

ab4166 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab4166 please see the product overview.

TDP1 activity in whole cell extracts. Whole cell extracts (WCE) generated from HEK293 transfected with pCI empty vector or pCI vector for human TDP1 overexpression (A); wild-type or TDP1−/− mouse embryonic fibroblast (MEF)(C); and TDP2−/− DT40 cells containing either pcDNA3.1-HisC empty vector or pcDNA3.1-HisC vector for human TDP2 overexpression (D) were used as the TDP1 source in the TDP1 fluorescent assay. The 15 μL reactions contained the indicated amounts of WCE. The reaction was carried out at room temperature for 10 min and terminated by addition of quench reagent. Relative fluorescence is shown and was calculated against the fluorescence obtained for 6.25 pM recombinant WT human TDP1 protein for each of three independent experiments. Error bars represent the standard deviation. WCEs generated from HEK293 transfected with pCI empty vector or pCI vector for human TDP1 overexpression (B) and TDP2−/− DT40 containing either pcDNA3.1-HisC empty vector or pcDNA3.1-HisC vector for human TDP2 expression (E) were separated by 10% SDS-PAGE, followed by Western blot analysis using antibodies against human TDP2 and actin.

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