Rabbit Polyclonal TDP43 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human TARDBP aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Tested | Expected |
Rat | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/100 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed:23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed:23519609, PubMed:24240615, PubMed:24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed:21358640, PubMed:29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed:28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed:30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed:19765185, PubMed:23398327). Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed:30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed:27123980). Negatively regulates the expression of CDK6 (PubMed:19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed:25678563).
TDP43, TDP43, TARDBP, TAR DNA-binding protein 43, TDP-43
Rabbit Polyclonal TDP43 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Rat, Human, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Human TARDBP aa 1-300.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
TDP43 also known as TAR DNA-binding protein 43 is a protein of approximately 43 kDa. It plays a role in various cellular processes primarily by binding to DNA and RNA. Researchers find TDP43 expressed mainly in neuronal tissues but it is present in other cell types as well. Known for its involvement in regulating gene expression and mRNA stability TDP43 interacts with other proteins within the nuclear compartment. The full function and activities of TDP43 are still under exploration but its importance in normal cellular functions is well recognized.
TDP43 acts as a regulator of RNA splicing and transcription by forming ribonucleoprotein complexes. The protein can bind to specific sequences in RNA helping in the proper processing and transport of mRNA. Furthermore TDP43 has a role in stress granule formation a cell response to stress. Researchers have identified that the protein undergoes various post-translational modifications which could influence its behavior and function within the cellular environment.
Several important pathways include TDP43 due to its functions in RNA metabolism. This protein contributes to the spliceosomal cycle and other pathways involved in mRNA processing. TDP43 interacts with proteins such as FUS and hnRNP which are also involved in RNA splicing and are essential for maintaining mRNA integrity. These relations make TDP43 an important player in regulating gene expression and protein synthesis.
The association of TDP43 with neurodegenerative diseases is significant. Its abnormal aggregation is linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). In these diseases TDP43 forms insoluble inclusions within neurons and glial cells. This mislocalization and aggregation can disrupt normal cellular function leading to cell death. In the context of ALS TDP43 often associates with proteins like SOD1 which are implicated in disease pathogenesis highlighting its role in neurodegeneration.
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Immunohistochemical analysis of paraffin-embedded Human Cal27 Xenograft tissue, labeling TARDBP with ab154047 at 1/100 dilution.
10% SDS PAGE
All lanes: Western blot - Anti-TDP43 antibody (ab154047) at 1/1000 dilution
Lane 1: A431 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Lane 3: HeLa whole cell lysate at 30 µg
Predicted band size: 44 kDa
10 % SDS-PAGE
All lanes: Western blot - Anti-TDP43 antibody (ab154047) at 1/3000 dilution
Lane 1: Neuro2A whole cell lysate at 30 µg
Lane 2: GL261 whole cell lysate at 30 µg
Lane 3: NIH 3T3 whole cell lysate at 30 µg
Lane 4: BCL1 whole cell lysate at 30 µg
Lane 5: RAW 264.7 whole cell lysate at 30 µg
Predicted band size: 44 kDa
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, labeling TARDBP with ab154047 at 1/200 dilution. Lower image is merged with DNA probe.
Immunohistochemical analysis of paraffin-embedded Rat brain tissue, labeling TARDBP with ab154047 at 1/500 dilution.
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