Anti-TDP43 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody (AB154047)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling TDP43 protein at nucleus using ab154047 at a 1/2000 dilution (Green). phalloidin, a cytoskeleton marker was stained by anti-phalloidin antibody at a 1/200 dilution (Red).

• IP

Supplier Data

Immunoprecipitation - Anti-TDP43 antibody (AB154047)

Samples were separated by 12% SDS-PAGE. The immunoprecipitated TARDBP protein was detected by ab154047 diluted at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TDP43 antibody (ab154047) at 2 µg

Lane 1:

HeLa whole cell lysate at 40 µg

Lane 2:

HeLa whole cell lysate with IgG control

Lane 3:

HeLa whole cell lysate

Secondary

All lanes:

Anti-rabbit IgG

Predicted band size: 45 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody (AB154047)

Immunohistochemical analysis of paraffin-embedded rat brain tissue stained for TDP43 protein at nucleus with ab154047 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody (AB154047)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue stained for TDP43 protein at nucleus with ab154047 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody (AB154047)

Immunofluorescence analysis of DIV10 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min labelling TDP43 protein using ab154047 at a 1/500 dilution (Green). Tau, stained by Tau antibody at a 1/500 dilution (Red). DAPI was used for nuclear staining (Blue).

• WB

Supplier Data

Western blot - Anti-TDP43 antibody (AB154047)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-TDP43 antibody (ab154047) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

HeLa whole cell lysate at 30 µg

Lane 4:

HepG2 whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false

• WB

Supplier Data

Western blot - Anti-TDP43 antibody (AB154047)

10 % SDS-PAGE

All lanes:

Western blot - Anti-TDP43 antibody (ab154047) at 1/3000 dilution

Lane 1:

Neuro2A whole cell lysate at 30 µg

Lane 2:

GL261 whole cell lysate at 30 µg

Lane 3:

NIH 3T3 whole cell lysate at 30 µg

Lane 4:

BCL1 whole cell lysate at 30 µg

Lane 5:

RAW 264.7 whole cell lysate at 30 µg

Predicted band size: 44 kDa

false

• WB

Supplier Data

Western blot - Anti-TDP43 antibody (AB154047)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-TDP43 antibody (ab154047) at 1/2000 dilution

Lane 1:

PC-12 whole cell extract at 30 µg

Lane 2:

Rat2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 45 kDa

false