JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB183752

Anti-TDP43 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal TDP43 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TARDBP aa 150-400.

View Alternative Names

TDP43, TARDBP, TAR DNA-binding protein 43, TDP-43

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody (AB183752)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody (AB183752)

Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling TARDBP with ab183752 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled Red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody (AB183752)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody (AB183752)

Immunohistochemical analysis of paraffin-embedded rat brain tissue labeling TARDBP with ab183752 at 1/500 dilution.

Western blot - Anti-TDP43 antibody (AB183752)
  • WB

Supplier Data

Western blot - Anti-TDP43 antibody (AB183752)

10% SDS PAGE

All lanes:

Western blot - Anti-TDP43 antibody (ab183752) at 1/500 dilution

Lane 1:

HeLa whole cell lysate mock control at 30 µg

Lane 2:

HeLa whole cell lysate transfected with shTDP-43 at 30 µg

Predicted band size: 44 kDa

false

Western blot - Anti-TDP43 antibody (AB183752)
  • WB

Supplier Data

Western blot - Anti-TDP43 antibody (AB183752)

10% SDS PAGE

All lanes:

Western blot - Anti-TDP43 antibody (ab183752) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 44 kDa

false

Western blot - Anti-TDP43 antibody (AB183752)
  • WB

Supplier Data

Western blot - Anti-TDP43 antibody (AB183752)

10% SDS PAGE

All lanes:

Western blot - Anti-TDP43 antibody (ab183752) at 1/500 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TARDBP aa 150-400. The exact immunogen used to generate this antibody is proprietary information.

Q13148

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDP43 also known as TAR DNA-binding protein 43 is a protein of approximately 43 kDa. It plays a role in various cellular processes primarily by binding to DNA and RNA. Researchers find TDP43 expressed mainly in neuronal tissues but it is present in other cell types as well. Known for its involvement in regulating gene expression and mRNA stability TDP43 interacts with other proteins within the nuclear compartment. The full function and activities of TDP43 are still under exploration but its importance in normal cellular functions is well recognized.
Biological function summary

TDP43 acts as a regulator of RNA splicing and transcription by forming ribonucleoprotein complexes. The protein can bind to specific sequences in RNA helping in the proper processing and transport of mRNA. Furthermore TDP43 has a role in stress granule formation a cell response to stress. Researchers have identified that the protein undergoes various post-translational modifications which could influence its behavior and function within the cellular environment.

Pathways

Several important pathways include TDP43 due to its functions in RNA metabolism. This protein contributes to the spliceosomal cycle and other pathways involved in mRNA processing. TDP43 interacts with proteins such as FUS and hnRNP which are also involved in RNA splicing and are essential for maintaining mRNA integrity. These relations make TDP43 an important player in regulating gene expression and protein synthesis.

The association of TDP43 with neurodegenerative diseases is significant. Its abnormal aggregation is linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). In these diseases TDP43 forms insoluble inclusions within neurons and glial cells. This mislocalization and aggregation can disrupt normal cellular function leading to cell death. In the context of ALS TDP43 often associates with proteins like SOD1 which are implicated in disease pathogenesis highlighting its role in neurodegeneration.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed : 23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed : 23519609, PubMed : 24240615, PubMed : 24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed : 21358640, PubMed : 29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed : 28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed : 30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed : 19765185, PubMed : 23398327). Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed : 30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed : 27123980). Negatively regulates the expression of CDK6 (PubMed : 19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed : 25678563).
See full target information TARDBP
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3890 PubMed38719850

2024

The Shigella kinase effector OspG modulates host ubiquitin signaling to escape septin-cage entrapment.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Xian,Jiaqi Fu,Qinxin Zhang,Chuang Li,Yan-Bo Zhao,Zhiheng Tang,Yi Yuan,Ying Wang,Yan Zhou,Peter S Brzoic,Ning Zheng,Songying Ouyang,Zhao-Qing Luo,Xiaoyun Liu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB109535

Anti-TDP43 antibody [EPR5810]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-TDP43 antibody [EPR5810] (AB109535)

  • 5
  • 1
Primary Antibodies

AB193842

Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-TDP43 antibody [EPR5810] (AB193842)

  • 0
  • 1
Primary Antibodies

AB133547

Anti-TDP43 antibody [EPR5811]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR5811] (AB133547)

  • 4
  • 3
Primary Antibodies

AB185133

Anti-TDP43 antibody [EPR5810] - BSA and Azide free

BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR5810] - BSA and Azide free (AB185133)

  • 0
  • 0
Primary Antibodies

AB238443

Anti-TDP43 antibody [EPR18554] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443)

  • 0
  • 0
Primary Antibodies

AB208542

Alexa Fluor® 594 Anti-TDP43 antibody [EPR5811]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-TDP43 antibody [EPR5811] (AB208542)

  • 0
  • 0
Primary Antibodies

AB190963

Anti-TDP43 antibody [EPR18554]

KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] (AB190963)

  • 4
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 74

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com