Anti-TDP43 antibody

• WB

Project

Western blot - Anti-TDP43 antibody (AB41881)

All lanes:

Western blot - Anti-TDP43 antibody (ab41881) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate at 10 µg

Lane 3:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 4:

Jurkat nuclear extract lysate (<a href='/en-us/products/unavailable/jurkat-nuclear-extract-lysate-ab14844'>ab14844</a>) at 10 µg

Lane 5:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 6:

Western blot - Hep G2 nuclear extract lysate (<a href='/en-us/products/tissue-lysates/hep-g2-nuclear-extract-lysate-ab14660'>ab14660</a>) at 10 µg

Lane 7:

A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 8:

K-562 (Human) Nuclear Lysate - tumor cell line (<a href='/en-us/products/unavailable/k-562-human-nuclear-lysate-tumor-cell-line-ab29309'>ab29309</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 44 kDa

Observed band size: 47 kDa

false

• WB

Lab

Western blot - Anti-TDP43 antibody (AB41881)

Lane 1 : Wild-type HAP1 cell lysate (40 μg)
Lane 2 : TDP43 knockout HAP1 cell lysate (40 μg)
Lane 3 : HeLa cell lysate (40 μg)
Lane 4 : Jurkat cell lysate (40 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab41881 observed at 48 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab41881 was shown to specifically react with TDP43 when TDP43 knockout samples were used. Wild-type and TDP43 knockout samples were subjected to SDS-PAGE. ab41881 and ab8245 (loading control to GAPDH) were diluted at 1 μg/mL and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TDP43 antibody (ab41881)

Predicted band size: 44 kDa

false