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AB80608

Anti-TDP43 antibody

4

(1 Review)

|

(2 Publications)

Goat Polyclonal TDP43 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TARDBP aa 250-300.

View Alternative Names

TDP43, TARDBP, TAR DNA-binding protein 43, TDP-43

2 Images
Western blot - Anti-TDP43 antibody (AB80608)
  • WB

Supplier Data

Western blot - Anti-TDP43 antibody (AB80608)

All lanes:

Western blot - Anti-TDP43 antibody (ab80608) at 1 µg/mL

Lane 1:

Human cerebellum cell lysate at 35 µg

Lane 2:

Human frontal cotex cell lysate at 35 µg

Lane 3:

Human hippocampus cell lysate at 35 µg

Predicted band size: 44 kDa

false

Western blot - Anti-TDP43 antibody (AB80608)
  • WB

Unknown

Western blot - Anti-TDP43 antibody (AB80608)

All lanes:

Western blot - Anti-TDP43 antibody (ab80608) at 0.3 µg/mL

All lanes:

Jurkat cell lysate (in RIPA buffer) at 35 µg

Predicted band size: 44 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TARDBP aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

Q13148

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab80608 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDP43 also known as TAR DNA-binding protein 43 is a protein of approximately 43 kDa. It plays a role in various cellular processes primarily by binding to DNA and RNA. Researchers find TDP43 expressed mainly in neuronal tissues but it is present in other cell types as well. Known for its involvement in regulating gene expression and mRNA stability TDP43 interacts with other proteins within the nuclear compartment. The full function and activities of TDP43 are still under exploration but its importance in normal cellular functions is well recognized.
Biological function summary

TDP43 acts as a regulator of RNA splicing and transcription by forming ribonucleoprotein complexes. The protein can bind to specific sequences in RNA helping in the proper processing and transport of mRNA. Furthermore TDP43 has a role in stress granule formation a cell response to stress. Researchers have identified that the protein undergoes various post-translational modifications which could influence its behavior and function within the cellular environment.

Pathways

Several important pathways include TDP43 due to its functions in RNA metabolism. This protein contributes to the spliceosomal cycle and other pathways involved in mRNA processing. TDP43 interacts with proteins such as FUS and hnRNP which are also involved in RNA splicing and are essential for maintaining mRNA integrity. These relations make TDP43 an important player in regulating gene expression and protein synthesis.

The association of TDP43 with neurodegenerative diseases is significant. Its abnormal aggregation is linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). In these diseases TDP43 forms insoluble inclusions within neurons and glial cells. This mislocalization and aggregation can disrupt normal cellular function leading to cell death. In the context of ALS TDP43 often associates with proteins like SOD1 which are implicated in disease pathogenesis highlighting its role in neurodegeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed : 23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed : 23519609, PubMed : 24240615, PubMed : 24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed : 21358640, PubMed : 29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed : 28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed : 30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed : 19765185, PubMed : 23398327). Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed : 30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed : 27123980). Negatively regulates the expression of CDK6 (PubMed : 19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed : 25678563).
See full target information TARDBP
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 19:1558227 PubMed40772263

2025

Accumulation of TDP-43 causes karyopherin-α4 pathology that characterises amyotrophic lateral sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Manpreet Singh Atwal,Jerneja Nimac,Urša Čerček,Sarah Ricarda Goesch,Hannah Rebecca Goesch,Paraskevi Tziortzouda,Tiziana Ercolani,Anna Zatorska,Terouz Pasha,Ivo Carre,Jacqueline Mitchell,Claire Troakes,Bart Tummers,Vera Župunski,Boris Rogelj,Tibor Hortobágyi,Frank Hirth

Journal of neurochemistry 48:225-32 PubMed3025363

1987

Purification and amino terminal sequencing of human melanoma nerve growth factor receptor.

Applications

Unspecified application

Species

Unspecified reactive species

N Marano,B Dietzschold,J J Earley,G Schatteman,S Thompson,P Grob,A H Ross,M Bothwell,B F Atkinson,H Koprowski
View all publications
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