Anti-TDP43 antibody [DB9]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (AB254166)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TDP43 with ab254166 at 4.67μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human colon is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (AB254166)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TDP43 with ab254166 at 4.67μg/ml followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (AB254166)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TDP43 with ab254166 at 23.35μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on rat cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [DB9] (AB254166)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TDP43 with ab254166 at 23.25μg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on mouse cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• WB

Lab

Western blot - Anti-TDP43 antibody [DB9] (AB254166)

ab254166 was shown to react with TARDBP in wild-type HAP1 cells in Western blot with loss of signal observed in a TARDBP knockout cell line. Wild-type HAP1 and TARDBP knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab254166 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-TDP43 antibody [DB9] (ab254166) at 1/1000 dilution

Lane 1:

Wild-type HAP1 lysate at 50 µg

Lane 2:

TARDBP knock-out HAP1 lysate at 50 µg

false

• WB

Supplier Data

Western blot - Anti-TDP43 antibody [DB9] (AB254166)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1-4 : 103 seconds;
Lanes 5-8 : 48 seconds.

All lanes:

Western blot - Anti-TDP43 antibody [DB9] (ab254166) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Lane 3:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 4:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 5:

C6 (rat glial tumor glial cell), whole cell lysate at 10 µg

Lane 6:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg

Lane 7:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg

Lane 8:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

• ELISA

Supplier Data

ELISA - Anti-TDP43 antibody [DB9] (AB254166)

TDP43 = 5000 ng/mL.

ab254166 used at 0 - 10,000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG （H+L）secondary antibody was used at 1000ng/ml