Rabbit Recombinant Monoclonal TDP43 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Zebrafish samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135577.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443)")
![Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunocytochemistry-immunofluorescence-img15504.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443)")
![Flow Cytometry (Intracellular) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--flow-cytometry-intracellular-img119598.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443)")
![Immunoprecipitation - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunoprecipitation-img3673.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129933.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (AB238443)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Zebrafish | Expected | Tested | Expected | Expected | Expected |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Zebrafish | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed:23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed:23519609, PubMed:24240615, PubMed:24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed:21358640, PubMed:29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed:28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed:30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed:19765185, PubMed:23398327). Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed:30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed:27123980). Negatively regulates the expression of CDK6 (PubMed:19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed:25678563).
Alternative names
TDP43, TARDBP, TAR DNA-binding protein 43, TDP-43
Recommended products
Rabbit Recombinant Monoclonal TDP43 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Zebrafish samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18554
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab238443 is the carrier-free version of Anti-TDP43 antibody [EPR18554] ab190963.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TDP43 also known as TAR DNA-binding protein 43 is a protein of approximately 43 kDa. It plays a role in various cellular processes primarily by binding to DNA and RNA. Researchers find TDP43 expressed mainly in neuronal tissues but it is present in other cell types as well. Known for its involvement in regulating gene expression and mRNA stability TDP43 interacts with other proteins within the nuclear compartment. The full function and activities of TDP43 are still under exploration but its importance in normal cellular functions is well recognized.
- Biological function summary
TDP43 acts as a regulator of RNA splicing and transcription by forming ribonucleoprotein complexes. The protein can bind to specific sequences in RNA helping in the proper processing and transport of mRNA. Furthermore TDP43 has a role in stress granule formation a cell response to stress. Researchers have identified that the protein undergoes various post-translational modifications which could influence its behavior and function within the cellular environment.
- Pathways
Several important pathways include TDP43 due to its functions in RNA metabolism. This protein contributes to the spliceosomal cycle and other pathways involved in mRNA processing. TDP43 interacts with proteins such as FUS and hnRNP which are also involved in RNA splicing and are essential for maintaining mRNA integrity. These relations make TDP43 an important player in regulating gene expression and protein synthesis.
- Associated diseases and disorders
The association of TDP43 with neurodegenerative diseases is significant. Its abnormal aggregation is linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). In these diseases TDP43 forms insoluble inclusions within neurons and glial cells. This mislocalization and aggregation can disrupt normal cellular function leading to cell death. In the context of ALS TDP43 often associates with proteins like SOD1 which are implicated in disease pathogenesis highlighting its role in neurodegeneration.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135577.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
Immunohistochemical analysis of paraffin-embedded Human endometrium carcinoma tissue labeling TDP43 with Anti-TDP43 antibody [EPR18554] ab190963 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on tumor cells of the endometrium carcinoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR18554] ab190963).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunocytochemistry-immunofluorescence-img15504.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling TDP43 with Anti-TDP43 antibody [EPR18554] ab190963 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-TDP43 antibody [EPR18554] ab190963 at 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR18554] ab190963).
![Flow Cytometry (Intracellular) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--flow-cytometry-intracellular-img119598.jpg "Flow Cytometry (Intracellular) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
Flow Cytometry (Intracellular) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling TDP43 with Anti-TDP43 antibody [EPR18554] ab190963 at 1/1000 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] -Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR18554] ab190963).
![Immunoprecipitation - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunoprecipitation-img3673.jpg "Immunoprecipitation - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
Immunoprecipitation - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
TDP43 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with Anti-TDP43 antibody [EPR18554] ab190963 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using Anti-TDP43 antibody [EPR18554] ab190963 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate, 10μg (Input).
Lane 2: Anti-TDP43 antibody [EPR18554] ab190963 IP in HeLa whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TDP43 antibody [EPR18554] ab190963 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
The band of ~35 kDa is the cleavage product from caspase activity (PMID:22659571, PMID:20736350).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR18554] ab190963).
All lanes: Immunoprecipitation - Anti-TDP43 antibody [EPR18554] (Anti-TDP43 antibody [EPR18554] ab190963)
Predicted band size: 44 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129933.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling TDP43 with Anti-TDP43 antibody [EPR18554] ab190963 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleus staining on normal Human pancreas is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR18554] ab190963).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunocytochemistry-immunofluorescence-img83197.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling TDP43 with Anti-TDP43 antibody [EPR18554] ab190963 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on K562 cell line.
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: Anti-TDP43 antibody [EPR18554] ab190963 at 1/2000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR18554] ab190963).
![Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--western-blot-img121097.jpg "Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
Lane 1: Wild type HAP1 whole cell lysate (20 μg)
Lane 2: TDP43 knockout HAP1 whole cell lysate (20 μg)
Lane 3: HeLa whole cell lysate (20 μg)Lanes 1 - 3: Merged signal (red and green). Green - Anti-TDP43 antibody [EPR18554] ab190963 observed at 48 kDa. Red - loading control, ab18058, observed at 130 kDa.
Anti-TDP43 antibody [EPR18554] ab190963 was shown to specifically react with TDP43 in wild type cells as signal was lost in TDP43 knockout cells. Wild-type and TDP43 knockout samples were subjected to SDS-PAGE. Anti-TDP43 antibody [EPR18554] ab190963 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR18554] ab190963).
All lanes: Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
Predicted band size: 44 kDa
![Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--western-blot-img438533.jpg "Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
This data was developed using Anti-TDP43 antibody [EPR18554] ab190963, the same antibody clone in a different buffer formulation.
Anti-TDP43 antibody [EPR18554] ab190963 was shown to react with TARDBP in wild-type HAP1 cells in Western blot with loss of signal observed in a TARDBP knockout cell line. Wild-type HAP1 and TARDBP knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-TDP43 antibody [EPR18554] ab190963 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Western blot - Anti-TDP43 antibody [EPR18554] (Anti-TDP43 antibody [EPR18554] ab190963) at 1/1000 dilution
Lane 1: Wild-type HAP1 lysate at 50 µg
Lane 2: TARDBP knock-out HAP1 lysate at 50 µg
![Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunocytochemistry-immunofluorescence-img438562.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)
This data was developed using Anti-TDP43 antibody [EPR18554] ab190963, the same antibody clone in a different buffer formulation.
Anti-TDP43 antibody [EPR18554] ab190963 was shown to react with TARDBP in wild-type HAP1 cells in immunocytochemistry with loss of signal observed in a TARDBP knockout cell line. Wild-type and knockout cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 1x PBS, 0.01% Triton X-100, 5% BSA, 5% NGS. The cells were then incubated with Anti-TDP43 antibody [EPR18554] ab190963 at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-rabbit secondary antibody to (Alexa Fluor® 555) at 0.5 ?g/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (knockout) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and knockout cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880).
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
![Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunocytochemistry-immunofluorescence-img83197.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
![Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--western-blot-img121097.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
![Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--western-blot-img438533.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
![Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443), expandable thumbnail](https://content.abcam.com/products/images/tdp43-antibody-epr18554-bsa-and-azide-free-ab238443--immunocytochemistry-immunofluorescence-img438562.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR18554] - BSA and Azide free (ab238443)")
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