Anti-TDP43 antibody [EPR5811] - BSA and Azide free

8 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR5811] - BSA and Azide free (ab248546)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling TDP43 with purified Anti-TDP43 antibody [EPR5811] ab133547 at 1/350 dilution (1.92 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR5811] ab133547).

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  Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR5811] - BSA and Azide free (ab248546)

  Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TDP43 with purified Anti-TDP43 antibody [EPR5811] ab133547 at 1/100 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% TritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR5811] ab133547).

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  Flow Cytometry (Intracellular) - Anti-TDP43 antibody [EPR5811] - BSA and Azide free (ab248546)

  Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling TDP43 with purified Anti-TDP43 antibody [EPR5811] ab133547 at 1/70 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS,BSA,glycerol,and sodium azide (Anti-TDP43 antibody [EPR5811] ab133547).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR5811] - BSA and Azide free (ab248546)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling TDP43 with purified Anti-TDP43 antibody [EPR5811] ab133547 at 1/350 dilution (1.92 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR5811] ab133547).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TDP43 antibody [EPR5811] - BSA and Azide free (ab248546)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling TDP43 with purified Anti-TDP43 antibody [EPR5811] ab133547 at 1/350 dilution (1.92 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR5811] ab133547).

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  Western blot - Anti-TDP43 antibody [EPR5811] - BSA and Azide free (ab248546)

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TDP43 antibody [EPR5811] ab133547).

  All lanes: Western blot - Anti-TDP43 antibody [EPR5811] (Anti-TDP43 antibody [EPR5811] ab133547) at 1/1000 dilution

  Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

  Lane 2: Human brain lysate at 20 µg

  Lane 3: Mouse brain lysate at 20 µg

  Lane 4: Rat brain lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Predicted band size: 44 kDa

  Observed band size: 44 kDa

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  Immunocytochemistry/ Immunofluorescence - Anti-TDP43 antibody [EPR5811] - BSA and Azide free (ab248546)

  This data was developed using Anti-TDP43 antibody [EPR5811] ab133547, the same antibody clone in a different buffer formulation.

  Anti-TDP43 antibody [EPR5811] ab133547 was shown to react with TARDBP in wild-type HAP1 cells in immunocytochemistry with loss of signal observed in a TARDBP knockout cell line. Wild-type and knockout cells were mixed and pelleted at a 1:1 ratio on coverslips. The cells were fixed with 4% paraformaldehyde (15 min) then permeabilized with 0.1% Triton X-100 (10min) and then blocked with 1x PBS, 0.01% Triton X-100, 5% BSA, 5% NGS. The cells were then incubated with Anti-TDP43 antibody [EPR5811] ab133547 at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat anti-rabbit secondary antibody to (Alexa Fluor® 555) at 0.5 ?g/ml. Acquisition of the green (wild-type), red (antibody staining) and far-red (knockout) channels was performed. Representative grayscale images of the red channel are shown. Wild-type and knockout cells are outlined with yellow and magenta dashed line, respectively. Schematic representation of the mosaic strategy used is shown on the bottom-right panel. Image was acquired with a Zeiss(LSM-880).
  
  These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

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  Western blot - Anti-TDP43 antibody [EPR5811] - BSA and Azide free (ab248546)

  This data was developed using Anti-TDP43 antibody [EPR5811] ab133547, the same antibody clone in a different buffer formulation.

  Anti-TDP43 antibody [EPR5811] ab133547 was shown to react with TARDBP in wild-type HAP1 cells in Western blot with loss of signal observed in a TARDBP knockout cell line. Wild-type HAP1 and TARDBP knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-TDP43 antibody [EPR5811] ab133547 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
  
  These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

  All lanes: Western blot - Anti-TDP43 antibody [EPR5811] (Anti-TDP43 antibody [EPR5811] ab133547) at 1/1000 dilution

  Lane 1: Wild-type HAP1 lysate at 50 µg

  Lane 2: TARDBP knock-out HAP1 lysate at 50 µg