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Knockout Tested Rabbit Monoclonal TDP43 phospho S409 + S410 antibody. Suitable for WB, Dot, I-ELISA and reacts with Human, Transfected cell lysate - Human, Synthetic peptide samples.

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Images

Dot Blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (AB314299), expandable thumbnail
  • Indirect ELISA - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (AB314299), expandable thumbnail
  • Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (AB314299), expandable thumbnail
  • Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (AB314299), expandable thumbnail
  • Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (AB314299), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBDotI-ELISAICC/IFIHC-PIPFlow Cyt (Intra)
Human
Tested
Expected
Expected
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide
Not recommended
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Transfected cell lysate - Human

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

500 ng/mL

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate - Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Transfected cell lysate - Human, Synthetic peptide, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell lysate - Human, Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Transfected cell lysate - Human, Synthetic peptide, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Transfected cell lysate - Human, Synthetic peptide, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    RNA-binding protein that is involved in various steps of RNA biogenesis and processing (PubMed:23519609). Preferentially binds, via its two RNA recognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantly localized within long introns and in the 3'UTR of mRNAs (PubMed:23519609, PubMed:24240615, PubMed:24464995). In turn, regulates the splicing of many non-coding and protein-coding RNAs including proteins involved in neuronal survival, as well as mRNAs that encode proteins relevant for neurodegenerative diseases (PubMed:21358640, PubMed:29438978). Plays a role in maintaining mitochondrial homeostasis by regulating the processing of mitochondrial transcripts (PubMed:28794432). Regulates also mRNA stability by recruiting CNOT7/CAF1 deadenylase on mRNA 3'UTR leading to poly(A) tail deadenylation and thus shortening (PubMed:30520513). In response to oxidative insult, associates with stalled ribosomes localized to stress granules (SGs) and contributes to cell survival (PubMed:23398327, PubMed:19765185). Participates also in the normal skeletal muscle formation and regeneration, forming cytoplasmic myo-granules and binding mRNAs that encode sarcomeric proteins (PubMed:30464263). Plays a role in the maintenance of the circadian clock periodicity via stabilization of the CRY1 and CRY2 proteins in a FBXL3-dependent manner (PubMed:27123980). Negatively regulates the expression of CDK6 (PubMed:19760257). Regulates the expression of HDAC6, ATG7 and VCP in a PPIA/CYPA-dependent manner (PubMed:25678563).

    Alternative names

    TAR DNA-binding protein 43, TDP-43, TDP43, TARDBP

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    Knockout Tested Rabbit Monoclonal TDP43 phospho S409 + S410 antibody. Suitable for WB, Dot, I-ELISA and reacts with Human, Transfected cell lysate - Human, Synthetic peptide samples.

    Alternative names

    TAR DNA-binding protein 43, TDP-43, TDP43, TARDBP

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle

    Notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Supplementary info

    Activity summary

    TDP43 also known as TAR DNA-binding protein 43 is a protein of approximately 43 kDa. It plays a role in various cellular processes primarily by binding to DNA and RNA. Researchers find TDP43 expressed mainly in neuronal tissues but it is present in other cell types as well. Known for its involvement in regulating gene expression and mRNA stability TDP43 interacts with other proteins within the nuclear compartment. The full function and activities of TDP43 are still under exploration but its importance in normal cellular functions is well recognized.

    Biological function summary

    TDP43 acts as a regulator of RNA splicing and transcription by forming ribonucleoprotein complexes. The protein can bind to specific sequences in RNA helping in the proper processing and transport of mRNA. Furthermore TDP43 has a role in stress granule formation a cell response to stress. Researchers have identified that the protein undergoes various post-translational modifications which could influence its behavior and function within the cellular environment.

    Pathways

    Several important pathways include TDP43 due to its functions in RNA metabolism. This protein contributes to the spliceosomal cycle and other pathways involved in mRNA processing. TDP43 interacts with proteins such as FUS and hnRNP which are also involved in RNA splicing and are essential for maintaining mRNA integrity. These relations make TDP43 an important player in regulating gene expression and protein synthesis.

    Associated diseases and disorders

    The association of TDP43 with neurodegenerative diseases is significant. Its abnormal aggregation is linked to amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). In these diseases TDP43 forms insoluble inclusions within neurons and glial cells. This mislocalization and aggregation can disrupt normal cellular function leading to cell death. In the context of ALS TDP43 often associates with proteins like SOD1 which are implicated in disease pathogenesis highlighting its role in neurodegeneration.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    6 product images

    • Dot Blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299), expandable thumbnail

      Dot Blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299)

      Dot blot analysis of TDP43 ( phospho S409 + pS410 ) using ab314299 at 1:1000 (0.461 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
      Exposure time: 180 seconds
      Blocking and diluting buffer and concentration: 5% NFDM/TBST

    • Indirect ELISA - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299), expandable thumbnail

      Indirect ELISA - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299)

      Indirect ELISA analysis of ab314299 at 500-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
      Antigen: TDP43 (phospho S410) peptide,TDP43 (phospho S409) peptide,TDP43 (phospho S409+S410) peptide.
      Antigen concentration: 50 ng/ml

    • Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299), expandable thumbnail

      Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      The identity of the higher MW bands above 45kDa are unknown.
      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

      All lanes: Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299) at 1/1000 dilution

      Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line) were treated with 1 uM Okadaic Acid for 60 minutes whole cell lysate at 20 µg

      Lane 2: TDP43 knockout HAP1 were treated with 1 uM Okadaic Acid for 60 minutes whole cell lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 45 kDa

      Exposure time: 147s

    • Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299), expandable thumbnail

      Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
      In Western blot, Anti-TDP43 antibody [EPR18554-55] (Anti-TDP43 antibody [EPR18554] ab190963) staining at 1/1000 dilution
      In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution

      All lanes: Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299) at 1/1000 dilution

      Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate (untreated membrane) at 20 µg

      Lane 2: 293T cells transfected with a human TDP43 expression vector containing a His-tag, whole cell lysate (untreated membrane) at 20 µg

      Lane 3: 293T cells transfected with a human TDP43 expression vector containing a His-tag were treated with 1 uM Okadaic Acid for 60 minutes, whole cell lysate (untreated membrane) at 20 µg

      Lane 4: 293T cells transfected with a human TDP43 expression vector containing a His-tag were treated with 1 uM Okadaic Acid for 60 minutes, whole cell lysate (Alkaline phosphatase treated membrane) at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 50 kDa

      Exposure time: 15s

    • Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299), expandable thumbnail

      Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299)

      Blocking and diluting buffer and concentration: 5% NFDM/TBST
      The antibody specifically recognizes TDP43 diphosphorylated at S409 and S410. It does not cross-react with TDP43 singly phosphorylated at S409 or singly phosphorylated at S410.
      In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
      In Western blot, Anti-TDP43 antibody [EPR18554-55] (Anti-TDP43 antibody [EPR18554] ab190963) staining at 1/1000 dilution
      In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution

      All lanes: Western blot - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299) at 1/1000 dilution

      Lane 1: 293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a His-tag, whole cell lysate at 20 µg

      Lane 2: 293T cells transfected with a human wild-type TDP43 expression vector containing a His-tag, whole cell lysate at 20 µg

      Lane 3: 293T cells transfected with a human wild-type TDP43 expression vector containing a His-tag were treated with 1 uM Okadaic Acid for 60 minutes, whole cell lysate at 20 µg

      Lane 4: 293T cells transfected with a human TDP43 (S409A and S410A mutation) expression vector containing a His-tag were treated with 1 uM Okadaic Acid for 60 minutes, whole cell lysate at 20 µg

      Lane 5: 293T cells transfected with a human TDP43 (S409A mutation) expression vector containing a His-tag were treated with 1 uM Okadaic Acid for 60 minutes, whole cell lysate at 20 µg

      Lane 6: 293T cells transfected with a human TDP43 (S410A mutation) expression vector containing a His-tag were treated with 1 uM Okadaic Acid for 60 minutes, whole cell lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

      Observed band size: 50 kDa

      Exposure time: 26s

    • Indirect ELISA - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299), expandable thumbnail

      Indirect ELISA - Anti-TDP43 (phospho S409 + pS410) antibody [EPR24512-120] (ab314299)

      Indirect ELISA analysis of ab314299 at 500-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution.
      Antigen: TDP43 non-phospho peptide,TDP43 (phospho S409+S410) peptide.
      Antigen concentration: 50 ng/ml

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