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AB317727

Anti-TDRD3 antibody [RAB-C419]

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Human Recombinant Monoclonal TDRD3 antibody. Suitable for WB, Flow Cyt (Intra), ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TDRD3 aa 550 to C-terminus.

View Alternative Names

Tudor domain-containing protein 3, TDRD3

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TDRD3 antibody [RAB-C419] (AB317727)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TDRD3 antibody [RAB-C419] (AB317727)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of ab317727 (1 : 100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1 : 1500 dilution), showing nucleoli, nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right, top-bottom primary antibody/secondary, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Flow Cytometry (Intracellular) - Anti-TDRD3 antibody [RAB-C419] (AB317727)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TDRD3 antibody [RAB-C419] (AB317727)

Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of ab317727 (blue line) at a dilution of 1 : 100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1 : 1000.

Western blot - Anti-TDRD3 antibody [RAB-C419] (AB317727)
  • WB

Supplier Data

Western blot - Anti-TDRD3 antibody [RAB-C419] (AB317727)

Chimeric rabbit version of ab317727 was used for this WB. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Lane 1:

Western blot - Anti-TDRD3 antibody [RAB-C419] (ab317727) at 1 µg/mL

Lanes 2, 3 and 5:

Western blot - Anti-TDRD3 antibody [RAB-C419] (ab317727) at 0.3 µg/mL

Lanes 4 and 6:

Western blot - Anti-TDRD3 antibody [RAB-C419] (ab317727) at 0.1 µg/mL

Lane 1:

Human cerebellum tissue lysate (35ug protein in RIPA buffer)

Lane 2:

Human skeletal muscle tissue lysate (35ug protein in RIPA buffer)

Lane 3:

Human heart tissue lysate (35ug protein in RIPA buffer)

Lane 4:

Human placenta tissue lysate (35ug protein in RIPA buffer)

Lane 5:

Human testes tissue lysate (35ug protein in RIPA buffer)

Lane 6:

Human kidney tissue lysate (35ug protein in RIPA buffer)

Secondary

All lanes:

Anti-rabbit secondary antibody

Predicted band size: 73 kDa

false

Key facts

Host species

Human

Clonality

Monoclonal

Clone number

RAB-C419

Isotype

Unknown

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

Recombinant Fragment Protein within Human TDRD3 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H7E2

Specificity

This antibody recognizes TDRD3 (Tudor domain-containing protein 3). It binds to a folded domain, amino acids 1-455.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified by Immobilized Metal Affinity Chromatography.
Storage buffer
pH: 7.2 Preservative: 0.02% Proclin 300 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TDRD3 also known as Tudor domain-containing protein 3 executes important functions within the cell. It has a mass of approximately 75 kDa. TDRD3 is known for its expression in various tissues with higher levels found in brain and liver. Its structural makeup includes a Tudor domain which helps it recognize and bind methylated arginine residues on proteins. The presence of such a domain is vital for its role in mRNA regulation and protein interaction.
Biological function summary

TDRD3 influences several processes related to mRNA dynamics and gene expression. It forms a part of the transcriptional co-activator complex that interacts with the RNA polymerase II machinery. Through this TDRD3 enhances transcription elongation playing a role in gene expression regulation. Additionally it interacts with other proteins like FMRP and PRMT1 establishing a close association with RNA-binding processes and methylation activities.

Pathways

The role of TDRD3 integrates into notable biological mechanisms like the mRNA processing and DNA damage response pathways. In the mRNA processing pathway TDRD3 collaborates with proteins such as CBC (cap-binding complex) which are essential for the maturation of mRNA. In the DNA damage response pathway TDRD3 works alongside factors like TopBP1 contributing to the activation and proper functioning of repair machinery.

Several insights link TDRD3 to cancer and mental disorders like Fragile X syndrome. Its interaction with FMRP known for its connection to Fragile X syndrome indicates a potential role in neural development abnormalities. Moreover altered TDRD3 expression and function in cancer supports its relevance to tumor progression often in coordination with proteins involved in the DNA repair pathway such as TopBP1. Understanding these associations aids in targeting TDRD3 for therapeutic applications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins (PubMed : 15955813). Plays a role in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins. In nucleus, acts as a coactivator : recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci (PubMed : 21172665). In cytoplasm, acts as an antiviral factor that participates in the assembly of stress granules together with G3BP1 (PubMed : 35085371).
See full target information TDRD3
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Product promise

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