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AB68886

Anti-TdT antibody [41A]

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(1 Publication)

Mouse Monoclonal TDT antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [41A] (AB68886)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [41A] (AB68886)

ab68886 (1 : 100) staining TdT in human thymus tissue by immunohistochemistry using formalin fixed, paraffin embedded tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [41A] (AB68886)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TdT antibody [41A] (AB68886)

ab68886 (1 : 100) staining human TdT in human thymus by immunohistochemistry using formalin fixed, paraffin embedded tissue.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

41A

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Purification notes
This antibody was purified using a AKTA prime.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target protein TdT also known as terminal deoxynucleotidyl transferase or DNTT is an enzyme with a mass of approximately 58 kDa. TdT is specifically expressed in the thymus and bone marrow during early B and T lymphocyte development. This enzyme adds nucleotides to the 3’ ends of DNA contributing to the diversity of the immune response by introducing variability at the junctions of gene segments.
Biological function summary

Terminal deoxynucleotidyl transferase plays an important role in the adaptive immune system. By adding random nucleotides to the V(D)J regions during the development of T and B cells it enhances the variability of antigen receptors. Although TdT does not form part of a larger protein complex its activity directly influences the effectiveness of antigen recognition by lymphocytes important for immune surveillance and response.

Pathways

Terminal deoxynucleotidyl transferase fits into the V(D)J recombination pathway which is essential for the assembly of diverse antigen receptor genes in immature lymphocytes. This enzyme works in conjunction with proteins like RAG1/RAG2 which initiate the DNA cleavage process necessary for recombination and with DNA ligase IV for the joining stage. Enhanced diversity generated by TdT's activity substantiates an effective and adaptable immune response.

TdT is associated with lymphoid malignancies such as acute lymphoblastic leukemia (ALL) where its expression acts as a diagnostic marker. Overexpression or dysregulation of TdT often found in these leukemias correlates with abnormal lymphocyte proliferation. TdT involvement in these disorders is linked to abnormal p53 pathways as p53 mutation frequently occurs alongside abnormal TdT activity suggesting a connection to cellular growth and survival dysregulation in affected cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.
See full target information DNTT

Alternative Names

TDT, DNTT, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase
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Publications (1)

Recent publications for all applications. Explore the full listand refine your search

The Journal of clinical investigation 122:1403-15 PubMed22378041

2012

Evidence for a stepwise program of extrathymic T cell development within the human tonsil.

Applications

IHC

Species

Human

Susan McClory,Tiffany Hughes,Aharon G Freud,Edward L Briercheck,Chelsea Martin,Anthony J Trimboli,Jianhua Yu,Xiaoli Zhang,Gustavo Leone,Gerard Nuovo,Michael A Caligiuri
View all publications
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Product promise

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