Anti-TdT antibody [EPR2976Y]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TdT antibody [EPR2976Y] (AB76544)

Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TdT with purified ab76544 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] (AB76544)

Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling TdT with Purified ab76544 at 1 : 100 dilution (1.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IP

Unknown

Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (AB76544)

ab76544 (purified) at 1 : 20 dilution (2μg) immunoprecipitating TdT in Jurkat (human acute T cell leukemia) whole cell lysate.

Lane 1 (input) : Jurkat (human acute T cell leukemia) whole cell lysate 10μg
Lane 2 (+) : ab76544 & Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76544 in Jurkat (human acute T cell leukemia) whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (ab76544)

Predicted band size: 59 kDa

false

• WB

Lab

Western blot - Anti-TdT antibody [EPR2976Y] (AB76544)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TdT antibody [EPR2976Y] (ab76544) at 1/2000 dilution

Lane 1:

Jurkat (human acute T cell leukemia) whole cell lysates at 15 µg

Lane 2:

Mouse thymus lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 59 kDa

false

• WB

Unknown

Western blot - Anti-TdT antibody [EPR2976Y] (AB76544)

All lanes:

Western blot - Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution

Lane 1:

Human thymus lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 59 kDa

Observed band size: 24 kDa,35 kDa,58 kDa

false

• WB

Lab

Western blot - Anti-TdT antibody [EPR2976Y] (AB76544)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution

All lanes:

Human fetal thymus lysates at 15 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 59 kDa

false

• WB

Lab

Western blot - Anti-TdT antibody [EPR2976Y] (AB76544)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution

All lanes:

Rat thymus lysates at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 59 kDa

false