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Rabbit Recombinant Monoclonal TDT antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 2 publications.

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Images

Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (AB76544), expandable thumbnail
  • Western blot - Anti-TdT antibody [EPR2976Y] (AB76544), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TdT antibody [EPR2976Y] (AB76544), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] (AB76544), expandable thumbnail
  • Western blot - Anti-TdT antibody [EPR2976Y] (AB76544), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/2000
Notes

-

Species
Human
Dilution info
1/2000
Notes

-

Species
Mouse
Dilution info
1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.

Alternative names

TDT, DNTT, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase

Recommended products

Rabbit Recombinant Monoclonal TDT antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2976Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The target protein TdT also known as terminal deoxynucleotidyl transferase or DNTT is an enzyme with a mass of approximately 58 kDa. TdT is specifically expressed in the thymus and bone marrow during early B and T lymphocyte development. This enzyme adds nucleotides to the 3’ ends of DNA contributing to the diversity of the immune response by introducing variability at the junctions of gene segments.

Biological function summary

Terminal deoxynucleotidyl transferase plays an important role in the adaptive immune system. By adding random nucleotides to the V(D)J regions during the development of T and B cells it enhances the variability of antigen receptors. Although TdT does not form part of a larger protein complex its activity directly influences the effectiveness of antigen recognition by lymphocytes important for immune surveillance and response.

Pathways

Terminal deoxynucleotidyl transferase fits into the V(D)J recombination pathway which is essential for the assembly of diverse antigen receptor genes in immature lymphocytes. This enzyme works in conjunction with proteins like RAG1/RAG2 which initiate the DNA cleavage process necessary for recombination and with DNA ligase IV for the joining stage. Enhanced diversity generated by TdT's activity substantiates an effective and adaptable immune response.

Associated diseases and disorders

TdT is associated with lymphoid malignancies such as acute lymphoblastic leukemia (ALL) where its expression acts as a diagnostic marker. Overexpression or dysregulation of TdT often found in these leukemias correlates with abnormal lymphocyte proliferation. TdT involvement in these disorders is linked to abnormal p53 pathways as p53 mutation frequently occurs alongside abnormal TdT activity suggesting a connection to cellular growth and survival dysregulation in affected cells.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (ab76544), expandable thumbnail

    Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (ab76544)

    ab76544 (purified) at 1:20 dilution (2μg) immunoprecipitating TdT in Jurkat (human acute T cell leukemia) whole cell lysate.

    Lane 1 (input): Jurkat (human acute T cell leukemia) whole cell lysate 10μg
    Lane 2 (+): ab76544 & Jurkat (human acute T cell leukemia) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab76544 in Jurkat (human acute T cell leukemia) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (ab76544)

    Predicted band size: 59 kDa

  • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544), expandable thumbnail

    Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution

    All lanes: Human fetal thymus lysates at 15 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 59 kDa

  • Flow Cytometry (Intracellular) - Anti-TdT antibody [EPR2976Y] (ab76544), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TdT antibody [EPR2976Y] (ab76544)

    Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TdT with purified ab76544 at 1/20 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] (ab76544), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] (ab76544)

    Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling TdT with Purified ab76544 at 1:100 dilution (1.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544), expandable thumbnail

    Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-TdT antibody [EPR2976Y] (ab76544) at 1/2000 dilution

    Lane 1: Jurkat (human acute T cell leukemia) whole cell lysates at 15 µg

    Lane 2: Mouse thymus lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 59 kDa

  • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544), expandable thumbnail

    Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)

    Blocking and diluting buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution

    All lanes: Rat thymus lysates at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 59 kDa

  • Western blot - Anti-TdT antibody [EPR2976Y] (ab76544), expandable thumbnail

    Western blot - Anti-TdT antibody [EPR2976Y] (ab76544)

    All lanes: Western blot - Anti-TdT antibody [EPR2976Y] (ab76544) at 1/10000 dilution

    Lane 1: Human thymus lysate at 10 µg

    Lane 2: Jurkat lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 59 kDa

    Observed band size: 24 kDa, 35 kDa, 58 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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