Anti-TdT antibody [EPR2976Y] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TdT antibody [EPR2976Y] - BSA and Azide free (AB232378)

Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TdT with purified ab76544 at 1/20 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76544).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TdT antibody [EPR2976Y] - BSA and Azide free (AB232378)

Immunocytochemistry/ Immunofluorescence analysis of MOLT-4 (Human lymphoblastic leukemia T lymphoblast) cells labeling TdT with Purified ab76544 at 1 : 100 dilution (1.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76544).

• IP

Unknown

Immunoprecipitation - Anti-TdT antibody [EPR2976Y] - BSA and Azide free (AB232378)

ab76544 (purified) at 1 : 20 dilution (2μg) immunoprecipitating TdT in Jurkat (human acute T cell leukemia) whole cell lysate.

Lane 1 (input) : Jurkat (human acute T cell leukemia) whole cell lysate 10μg
Lane 2 (+) : ab76544 & Jurkat (human acute T cell leukemia) whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab76544 in Jurkat (human acute T cell leukemia) whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76544).

All lanes:

Immunoprecipitation - Anti-TdT antibody [EPR2976Y] (<a href='/en-us/products/primary-antibodies/tdt-antibody-epr2976y-ab76544'>ab76544</a>)

Predicted band size: 59 kDa

false