Mouse Monoclonal TEA domain family member 2/ETF antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TEAD2.
Images
![Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (AB54374), expandable thumbnail](https://content.abcam.com/products/images/tea-domain-family-member-2-etf-antibody-404c5a-ab54374--western-blot-img98802.gif "Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (AB54374)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human TEAD2. The exact immunogen used to generate this antibody is proprietary information. Database link Q15562
Reactivity data
Select an application| WB | |
|---|---|
| Recombinant fragment - Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.
Alternative names
TEF4, TEAD2, Transcriptional enhancer factor TEF-4, TEA domain family member 2, TEAD-2
Recommended products
Mouse Monoclonal TEA domain family member 2/ETF antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TEAD2.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human TEAD2. The exact immunogen used to generate this antibody is proprietary information. Database link Q15562
- Clone number
- 404C5a
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
ab54374 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TEA domain family member 2 (also known as ETF or TEAD2) is a transcription factor belonging to the TEAD protein family with a molecular mass around 47 kDa. TEAD2 plays an important mechanical role in regulating gene expression by binding to enhancer regions within the DNA. You will find TEAD2 predominantly expressed in tissues such as the brain heart and skeletal muscle. This expression pattern indicates its involvement in critical cellular activities.
- Biological function summary
TEAD2 functions as a participant in the transcriptional activation of target genes by forming complexes with other cofactors such as YAP and TAZ. This complex formation facilitates the modulation of cell proliferation differentiation and apoptosis. TEAD2 activation leads to transcriptional regulation of genes promoting these cellular processes signifying its importance in maintaining tissue homeostasis and development.
- Pathways
TEAD2 participates importantly in the Hippo signaling pathway which plays a role in organ size determination and tumor suppression. The TEAD2 pathway interaction occurs via its association with YAP and TAZ proteins which transmit signals from cell surface receptors to the nucleus. Additionally involvement in Wnt signaling connects TEAD2 to a network of proteins regulating embryonic development and adult tissue homeostasis further highlighting its pathway integration.
- Associated diseases and disorders
Dysregulation of TEAD2 activity has implications in oncological processes especially concerning tumor growth and metastasis. TEAD2's association with YAP and TAZ is significant in cancer biology due to their role in constitutive pathway activation which can lead to uncontrolled cell growth. Abnormal TEAD2 signaling may also influence diseases linked to developmental malformations because of its regulatory activity in important developmental pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374)
The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen.
All lanes: Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374)
All lanes: immunizing recombinant protein
Predicted band size: 49 kDa
Observed band size: 36 kDa
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