AB54374

Anti-TEA domain family member 2/ETF antibody [404C5a]

Name: Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374) | Abcam
Brand: Abcam Limited
SKU: AB54374
Rating: 4 (1 reviews)

(1 Review)

(1 Publication)

Mouse Monoclonal TEA domain family member 2/ETF antibody. Suitable for WB and reacts with Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TEAD2.

View Alternative Names

TEF4, TEAD2, Transcriptional enhancer factor TEF-4, TEA domain family member 2, TEAD-2

1 Images

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Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (AB54374)

The molecular weight of the band on the Western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant protein fragment was used as the test antigen.

All lanes:

Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (ab54374)

All lanes:

immunizing recombinant protein

Predicted band size: 49 kDa

Observed band size: 36 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

404C5a

Isotype

IgG1

Carrier free

Applications

applications

Immunogen

Recombinant Fragment Protein within Human TEAD2. The exact immunogen used to generate this antibody is proprietary information.

Q15562

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab54374 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TEA domain family member 2 (also known as ETF or TEAD2) is a transcription factor belonging to the TEAD protein family with a molecular mass around 47 kDa. TEAD2 plays an important mechanical role in regulating gene expression by binding to enhancer regions within the DNA. You will find TEAD2 predominantly expressed in tissues such as the brain heart and skeletal muscle. This expression pattern indicates its involvement in critical cellular activities.

Biological function summary

TEAD2 functions as a participant in the transcriptional activation of target genes by forming complexes with other cofactors such as YAP and TAZ. This complex formation facilitates the modulation of cell proliferation differentiation and apoptosis. TEAD2 activation leads to transcriptional regulation of genes promoting these cellular processes signifying its importance in maintaining tissue homeostasis and development.

Pathways

TEAD2 participates importantly in the Hippo signaling pathway which plays a role in organ size determination and tumor suppression. The TEAD2 pathway interaction occurs via its association with YAP and TAZ proteins which transmit signals from cell surface receptors to the nucleus. Additionally involvement in Wnt signaling connects TEAD2 to a network of proteins regulating embryonic development and adult tissue homeostasis further highlighting its pathway integration.

Dysregulation of TEAD2 activity has implications in oncological processes especially concerning tumor growth and metastasis. TEAD2's association with YAP and TAZ is significant in cancer biology due to their role in constitutive pathway activation which can lead to uncontrolled cell growth. Abnormal TEAD2 signaling may also influence diseases linked to developmental malformations because of its regulatory activity in important developmental pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.

See full target information TEAD2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 7:49042-49064 PubMed27119498

2016

Vitamin D3 suppresses morphological evolution of the cribriform cancerous phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Ravi K Deevi,Jane McClements,Karen D McCloskey,Aliya Fatehullah,Dorota Tkocz,Arman Javadi,Robyn Higginson,Victoria Marsh Durban,Marnix Jansen,Alan Clarke,Maurice B Loughrey,Frederick C Campbell

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TEA domain family member 2/ETF antibody [404C5a]

Western blot - Anti-TEA domain family member 2/ETF antibody [404C5a] (AB54374)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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