Anti-TEAD4 antibody [5H3] (ab58310) is a mouse monoclonal antibody that is used to detect TEAD4 in Western Blot. Suitable for Human samples.
- Over 90 publications
- Trusted since 2007
pH: 7.4
Constituents: 91% Water, 8% Sodium chloride, 0.6% Disodium hydrogenorthophosphate, 0.2% Potassium chloride, 0.2% Potassium phosphate monobasic
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Human | Tested |
Recombinant full length protein | Tested |
Species | Dilution info | Notes |
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Species Recombinant full length protein, Human | Dilution info - | Notes - |
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Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and non-cooperatively to the Sph and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription. Binds to the M-CAT motif.
RTEF1, TCF13L1, TEF3, TEAD4, Transcriptional enhancer factor TEF-3, TEA domain family member 4, Transcription factor 13-like 1, Transcription factor RTEF-1, TEAD-4
Anti-TEAD4 antibody [5H3] (ab58310) is a mouse monoclonal antibody that is used to detect TEAD4 in Western Blot. Suitable for Human samples.
- Over 90 publications
- Trusted since 2007
pH: 7.4
Constituents: 91% Water, 8% Sodium chloride, 0.6% Disodium hydrogenorthophosphate, 0.2% Potassium chloride, 0.2% Potassium phosphate monobasic
TEAD4 also known as TEF-3 or TEF is a transcription factor that plays a significant mechanical role in gene regulation. It belongs to the TEAD family of proteins. TEAD4 has an approximate mass of 48 kDa and is ubiquitously expressed in various tissues. It interacts with different cofactors to regulate gene expression and influences several cellular processes. The protein's expression is especially noted in cancerous tissues emphasizing its role in cellular growth and proliferation.
TEAD4 is important for embryonic development and cell fate determination. It forms a complex with YAP (Yes-associated protein) to regulate transcriptional activities. The TEAD4-YAP complex binds to DNA driving the expression of genes involved in cell proliferation and survival. TEAD4 also coordinates with _tef_1 and several other transcription factors to regulate genes necessary for development and differentiation influencing processes such as muscle and neural tissue formation.
TEAD4 is primarily involved in the Hippo signaling pathway known to regulate organ size by controlling cell proliferation and apoptosis. TEAD4 interacts with proteins like YAP and TAZ which play integral roles within this pathway. The Hippo pathway influences cell growth and TEAD4 as a mediator of this action contributes to the balance between cell proliferation and apoptosis. TEAD4's role extends to pathways involving cell cycle regulation and response to cellular stress.
TEAD4 exhibits significant relevance in cancer and cardiovascular diseases. Overexpression or dysregulation of TEAD4 often through its interaction with YAP is linked with tumorigenesis in breast and liver cancers. It promotes cancer cell proliferation migration and invasion. In the cardiovascular system TEAD4 impacts heart development and function with implications in congenital heart defects. Its interaction with proteins like _tef_1 also highlights its role in these diseases making it a potential therapeutic target.
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False colour image of Western blot: Anti-TEAD4 antibody [5H3] staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab58310 was shown to bind specifically to TEAD4. A band was observed at 48 kDa in wild-type A549 cell lysates with no signal observed at this size in TEAD4 CRISPR-Cas9 edited cell line Human TEAD4 knockout A549 cell line ab277855. The band observed in the CRISPR-Cas9 edited lysate lane below 48 kDa is likely to represent a truncated form of TEAD4. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and TEAD4 CRISPR-Cas9 edited A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-TEAD4 antibody [5H3] (ab58310) at 1 µg
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: TEAD4 CRISPR-Cas9 edited A549 cell lysate at 20 µg
Lane 2: Western blot - Human TEAD4 knockout A549 cell line (Human TEAD4 knockout A549 cell line ab277855) at 20 µg
Lane 3: T-47D cell lysate at 20 µg
Performed under reducing conditions.
Observed band size: 48 kDa
TEAD4 antibody (ab58310) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-TEAD4 antibody [5H3] (ab58310)
Predicted band size: 48 kDa
Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-TEAD4 antibody [5H3] (ab58310) at 1/1000 dilution
Lane 1: TEAD1 Human Recombinant Protein at 20 µg
Lane 2: TEAD2 Human Recombinant Protein at 20 µg
Lane 3: TEAD3 Human Recombinant Protein at 20 µg
Lane 4: TEAD4 Human Recombinant Protein at 20 µg
All lanes: Goat Anti-mouse IgG at 1/5000 dilution
Predicted band size: 48 kDa
Observed band size: 50-75 kDa
Exposure time: 180s
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