Anti-TEAD4 antibody [5H3]

• WB

Lab

Western blot - Anti-TEAD4 antibody [5H3] (AB58310)

False colour image of Western blot : Anti-TEAD4 antibody [5H3] staining at 1 ug/ml, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab58310 was shown to bind specifically to TEAD4. A band was observed at 48 kDa in wild-type A549 cell lysates with no signal observed at this size in TEAD4 CRISPR-Cas9 edited cell line ab277855. The band observed in the CRISPR-Cas9 edited lysate lane below 48 kDa is likely to represent a truncated form of TEAD4. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and TEAD4 CRISPR-Cas9 edited A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-TEAD4 antibody [5H3] (ab58310) at 1 µg

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

TEAD4 CRISPR-Cas9 edited A549 cell lysate at 20 µg

Lane 2:

Western blot - Human TEAD4 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-tead4-knockout-a549-cell-line-ab277855'>ab277855</a>) at 20 µg

Lane 3:

T-47D cell lysate at 20 µg

Observed band size: 48 kDa

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• WB

Unknown

Western blot - Anti-TEAD4 antibody [5H3] (AB58310)

TEAD4 antibody (ab58310) at 1ug/lane + HeLa cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-TEAD4 antibody [5H3] (ab58310)

Predicted band size: 48 kDa

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• WB

Supplier Data

Western blot - Anti-TEAD4 antibody [5H3] (AB58310)

Blocking/Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TEAD4 antibody [5H3] (ab58310) at 1/1000 dilution

Lane 1:

TEAD1 Human Recombinant Protein at 20 µg

Lane 2:

TEAD2 Human Recombinant Protein at 20 µg

Lane 3:

TEAD3 Human Recombinant Protein at 20 µg

Lane 4:

TEAD4 Human Recombinant Protein at 20 µg

Secondary

All lanes:

Goat Anti-mouse IgG at 1/5000 dilution

Predicted band size: 48 kDa

Observed band size: 50-75 kDa

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Exposure time: 180s