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Rabbit Recombinant Monoclonal TEF1/TEAD-1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Rat, Mouse, Human samples.

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Images

Western blot - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (AB219647), expandable thumbnail
  • Immunoprecipitation - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (AB219647), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (AB219647), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (AB219647), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (AB219647), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Not recommended
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Expected
Not recommended
Tested
Rat
Tested
Not recommended
Expected
Not recommended
Tested

Tested
Tested

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat, Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.

Alternative names

Transcriptional enhancer factor TEF-1, NTEF-1, Protein GT-IIC, TEA domain family member 1, Transcription factor 13, TEAD-1, TCF-13, TEF1, TCF13, TEAD1

Recommended products

Rabbit Recombinant Monoclonal TEF1/TEAD-1 antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Rat, Mouse, Human samples.

Alternative names

Transcriptional enhancer factor TEF-1, NTEF-1, Protein GT-IIC, TEA domain family member 1, Transcription factor 13, TEAD-1, TCF-13, TEF1, TCF13, TEAD1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR3967(2)

Purification technique

Affinity purification Protein A

Specificity

There is 71% homology between the antibody immunogen and the TEF5 protein. Preliminary ELISA data suggests weak cross-reactivity with TEF5, no cross-reactivity with TEF3 and TEF4.

Dissociation constant

1.95 x 10-10 M

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab219647 is the carrier-free version of Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647), expandable thumbnail

    Western blot - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533).

    Lanes 1 - 2: Merged signal (red and green). Green - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 was shown to react with TEF1/TEAD-1 in wild-type A549 cells in western blot with loss of signal observed in TEAD1 knockout sample. Wild-type and TEAD1 knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533) at 1/1000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: TEAD1 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 48 kDa

    Observed band size: 50 kDa

  • Immunoprecipitation - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647), expandable thumbnail

    Immunoprecipitation - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 (purified) at 1:80 dilution (2ug) immunoprecipitating TEF1/TEAD-1 in 293 (Human embryonic kidney epithelial cell) whole cell lysate.
    Lane 1 (input): 293 (Human embryonic kidney epithelial cell) whole cell lysate 10ug
    Lane 2 (+): Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 & 293 (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 in 293 (Human embryonic kidney epithelial cell) whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533).

    All lanes: Immunoprecipitation - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533)

    Predicted band size: 48 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue sections labeling TEF1/TEAD-1 with Purified Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 at 1:1000 dilution (1.63 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue sections labeling TEF1/TEAD-1 with Purified Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 at 1:1000 dilution (1.63 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labeling TEF1/TEAD-1 with Purified Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 at 1:1000 dilution (1.63 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry analysis of Paraffin Embedded Human placenta tissue labelling TEF1/TEAD-1 with unpurified Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 at 1/100. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    Immunohistochemistry analysis of Paraffin Embedded Human skeletal muscle tissue labelling TEF1/TEAD-1 with unpurified Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 at 1/100. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533).

  • OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647), expandable thumbnail

    OI-RD Scanning - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Western blot - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647), expandable thumbnail

    Western blot - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] - BSA and Azide free (ab219647)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 recognizes human TEAD1 and human TEAD3.

    All lanes: Western blot - Anti-TEF1/TEAD-1 antibody [EPR3967(2)] (Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533) at 1/5000 dilution

    Lane 1: His-GST-tagged human TEAD1 recombinant protein at 20 µg

    Lane 2: His-SUMO-tagged human TEAD3 recombinant protein at 20 µg

    Lane 3: His-tagged human TEAD4 recombinant protein at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 50 kDa, 70 kDa

    Exposure time: 10s

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    Anti-TEF1/TEAD-1 antibody [EPR3967(2)] ab133533 recognizes human TEAD1 and human TEAD3.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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