AB133646

Anti-Telethonin antibody [EPR8375]

Name: Anti-Telethonin antibody [EPR8375] (ab133646) | Abcam
Brand: Abcam Limited
SKU: AB133646
Price: 275 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(5 Publications)

Rabbit Recombinant Monoclonal Telethonin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

View Alternative Names

Telethonin, Titin cap protein, TCAP

4 Images

Lab

Western blot - Anti-Telethonin antibody [EPR8375] (AB133646)

Blocking buffer 5% NFDM/TBST

Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-Telethonin antibody [EPR8375] (ab133646) at 1/5000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

C2C12 cell lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Mouse muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Exposure time: 1min

Lab

Western blot - Anti-Telethonin antibody [EPR8375] (AB133646)

Blocking buffer 5% NFDM/TBST

Diluting buffer 5% NFDM/TBST

Lane 1:

Western blot - Anti-Telethonin antibody [EPR8375] (ab133646) at 1/500 dilution

Lane 2:

Western blot - Anti-Telethonin antibody [EPR8375] (ab133646) at 1/5000 dilution

Lane 1:

Rat Muscle tissue lysate at 15 µg

Lane 2:

Rat Heart tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Exposure time: 1min

Unknown

Western blot - Anti-Telethonin antibody [EPR8375] (AB133646)

All lanes:

Western blot - Anti-Telethonin antibody [EPR8375] (ab133646) at 1/1000 dilution

Lane 1:

Fetal muscle lysate at 10 µg

Lane 2:

Fetal heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

false

OI-RD Scanning - Anti-Telethonin antibody [EPR8375] (AB133646)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Telethonin antibody [EPR8375] (AB133646)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Telethonin antibody [EPR8375] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8375

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Telethonin also known as Tcap is a small protein with a molecular mass of approximately 19 kDa. It is an important component of the sarcomere in striated muscle cells primarily expressed in the heart and skeletal muscles. Telethonin resides at the Z-disc of the sarcomere where it interacts with titin one of the largest known proteins to maintain the structural integrity and elasticity of muscle tissues. This protein supports the mechanical stability of muscle fibers during contraction and relaxation by acting as a molecular spring.

Biological function summary

The function of telethonin expands beyond simple structural support. It acts as part of the protein complex at the Z-disc important for maintaining sarcomere architecture. This complex involves interactions not only with titin but also with other structural proteins such as actin and myotilin. Telethonin's presence helps coordinate the assembly and maintenance of the Z-disc contributing to muscle strength and resilience. It also plays a role in signaling pathways that regulate muscle protein gene expression and response to mechanical stress.

Pathways

Telethonin integrates into several significant signaling cascades. One major pathway involves the titin-telethonin interaction as part of the mechanical signaling responsible for muscle function and adaptation. Through its involvement with titin telethonin influences pathways linked to muscle hypertrophy and atrophy as well as sarcomere organization. Furthermore telethonin associates with signaling proteins like myostatin an important regulator of muscle growth modulating muscle size and adaptation to workload changes.

Telethonin's malfunction often shows links with cardiomyopathies and muscular dystrophies. Mutations in the telethonin gene are associated with limb-girdle muscular dystrophy type 2G a condition where muscle weakness and degeneration occur due to defects in the muscle fiber structure. Moreover telethonin's interaction with titin connects it to dilated cardiomyopathy a condition where the heart weakens and enlarges impairing its ability to pump effectively. Understanding telethonin’s role provides insights into these muscle-related diseases and offers potential therapeutic targets for intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Muscle assembly regulating factor. Mediates the antiparallel assembly of titin (TTN) molecules at the sarcomeric Z-disk.

See full target information TCAP

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 10: PubMed39804820

2025

Constitutive deletion of the obscurin-Ig58/59 domains induces atrial remodeling and Ca2+-based arrhythmogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Alyssa Grogan,Annie Brong,Humberto C Joca,Liron Boyman,Aaron D Kaplan,Christopher W Ward,Maura Greiser,Aikaterini Kontrogianni-Konstantopoulos

Methods in molecular biology (Clifton, N.J.) 2784:163-176 PubMed38502485

2024

Multiplexed Immunofluorescence and Single-Molecule RNA Fluorescence In Situ Hybridization in Mouse Skeletal Myofibers.

Applications

Unspecified application

Species

Unspecified reactive species

Lance T Denes,Chase P Kelley,Eric T Wang

Nature communications 12:6079 PubMed34707124

2021

Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Lance T Denes,Chase P Kelley,Eric T Wang

Neurogenetics 22:1-10 PubMed32761539

2020

Distal myopathy due to TCAP variants in four unrelated Chinese patients.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Lv,Fei Gao,Tingjun Dai,Dandan Zhao,Wei Jiang,Hongzhi Geng,Fuchen Liu,Pengfei Lin,Chuanzhu Yan

Scientific reports 9:6801 PubMed31043634

2019

Phosphodiesterase 5 inhibition improves contractile function and restores transverse tubule loss and catecholamine responsiveness in heart failure.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Lawless,Jessica L Caldwell,Emma J Radcliffe,Charlotte E R Smith,George W P Madders,David C Hutchings,Lori S Woods,Stephanie J Church,Richard D Unwin,Graeme J Kirkwood,Lorenz K Becker,Charles M Pearman,Rebecca F Taylor,David A Eisner,Katharine M Dibb,Andrew W Trafford

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Telethonin antibody [EPR8375]

Western blot - Anti-Telethonin antibody [EPR8375] (AB133646)

Western blot - Anti-Telethonin antibody [EPR8375] (AB133646)

Western blot - Anti-Telethonin antibody [EPR8375] (AB133646)

OI-RD Scanning - Anti-Telethonin antibody [EPR8375] (AB133646)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise