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Rabbit Recombinant Monoclonal Telethonin antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Not recommended
Tested
Not recommended
Mouse
Not recommended
Tested
Not recommended
Rat
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Target data

Function

Muscle assembly regulating factor. Mediates the antiparallel assembly of titin (TTN) molecules at the sarcomeric Z-disk.

Alternative names

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Rabbit Recombinant Monoclonal Telethonin antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR8375

Purification technique

Affinity purification

Dissociation constant

1.3 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab248591 is the carrier-free version of ab133646.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

The function of telethonin expands beyond simple structural support. It acts as part of the protein complex at the Z-disc important for maintaining sarcomere architecture. This complex involves interactions not only with titin but also with other structural proteins such as actin and myotilin. Telethonin's presence helps coordinate the assembly and maintenance of the Z-disc contributing to muscle strength and resilience. It also plays a role in signaling pathways that regulate muscle protein gene expression and response to mechanical stress.

Activity summary

Telethonin also known as Tcap is a small protein with a molecular mass of approximately 19 kDa. It is an important component of the sarcomere in striated muscle cells primarily expressed in the heart and skeletal muscles. Telethonin resides at the Z-disc of the sarcomere where it interacts with titin one of the largest known proteins to maintain the structural integrity and elasticity of muscle tissues. This protein supports the mechanical stability of muscle fibers during contraction and relaxation by acting as a molecular spring.

Pathways

Telethonin integrates into several significant signaling cascades. One major pathway involves the titin-telethonin interaction as part of the mechanical signaling responsible for muscle function and adaptation. Through its involvement with titin telethonin influences pathways linked to muscle hypertrophy and atrophy as well as sarcomere organization. Furthermore telethonin associates with signaling proteins like myostatin an important regulator of muscle growth modulating muscle size and adaptation to workload changes.

Associated diseases and disorders

Telethonin's malfunction often shows links with cardiomyopathies and muscular dystrophies. Mutations in the telethonin gene are associated with limb-girdle muscular dystrophy type 2G a condition where muscle weakness and degeneration occur due to defects in the muscle fiber structure. Moreover telethonin's interaction with titin connects it to dilated cardiomyopathy a condition where the heart weakens and enlarges impairing its ability to pump effectively. Understanding telethonin’s role provides insights into these muscle-related diseases and offers potential therapeutic targets for intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (ab248591), expandable thumbnail

    Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (ab248591)

    This data was developed using ab133646, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Lane 1: Western blot - Anti-Telethonin antibody [EPR8375] (AB133646) at 1/500 dilution

    Lane 2: Western blot - Anti-Telethonin antibody [EPR8375] (AB133646) at 1/5000 dilution

    Lane 1: Rat Muscle tissue lysate at 15 µg

    Lane 2: Rat Heart tissue lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 19 kDa

    Observed band size: 19 kDa

    Exposure time: 1min

    Blocking buffer 5% NFDM/TBST

    Diluting buffer 5% NFDM/TBST

  • Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (ab248591), expandable thumbnail

    Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (ab248591)

    This data was developed using ab133646, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Telethonin antibody [EPR8375] (AB133646) at 1/5000 dilution

    Lane 1: Human skeletal muscle tissue lysate at 20 µg

    Lane 2: C2C12 cell lysate at 20 µg

    Lane 3: Mouse heart tissue lysate at 20 µg

    Lane 4: Mouse muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 19 kDa

    Observed band size: 19 kDa

    Exposure time: 1min

    Blocking buffer 5% NFDM/TBST

    Diluting buffer 5% NFDM/TBST

  • Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (ab248591), expandable thumbnail

    Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (ab248591)

    This data was developed using ab133646, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Telethonin antibody [EPR8375] (AB133646) at 1/1000 dilution

    Lane 1: Fetal muscle lysate at 10 µg

    Lane 2: Fetal heart lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 19 kDa

  • OI-RD Scanning - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (ab248591), expandable thumbnail

    OI-RD Scanning - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (ab248591)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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