AB248591

Anti-Telethonin antibody [EPR8375] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Telethonin antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Telethonin, Titin cap protein, TCAP

4 Images

Lab

Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (AB248591)

This data was developed using ab133646, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Telethonin antibody [EPR8375] (<a href='/en-us/products/primary-antibodies/telethonin-antibody-epr8375-ab133646'>ab133646</a>) at 1/5000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

C2C12 cell lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Mouse muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Exposure time: 1min

Lab

Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (AB248591)

This data was developed using ab133646, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-Telethonin antibody [EPR8375] (<a href='/en-us/products/primary-antibodies/telethonin-antibody-epr8375-ab133646'>ab133646</a>) at 1/500 dilution

Lane 2:

Western blot - Anti-Telethonin antibody [EPR8375] (<a href='/en-us/products/primary-antibodies/telethonin-antibody-epr8375-ab133646'>ab133646</a>) at 1/5000 dilution

Lane 1:

Rat Muscle tissue lysate at 15 µg

Lane 2:

Rat Heart tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Exposure time: 1min

Unknown

Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (AB248591)

This data was developed using ab133646, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Telethonin antibody [EPR8375] (<a href='/en-us/products/primary-antibodies/telethonin-antibody-epr8375-ab133646'>ab133646</a>) at 1/1000 dilution

Lane 1:

Fetal muscle lysate at 10 µg

Lane 2:

Fetal heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

false

OI-RD Scanning - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (AB248591)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (AB248591)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-Telethonin antibody [EPR8375]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8375

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab248591 is the carrier-free version of ab133646.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Telethonin also known as Tcap is a small protein with a molecular mass of approximately 19 kDa. It is an important component of the sarcomere in striated muscle cells primarily expressed in the heart and skeletal muscles. Telethonin resides at the Z-disc of the sarcomere where it interacts with titin one of the largest known proteins to maintain the structural integrity and elasticity of muscle tissues. This protein supports the mechanical stability of muscle fibers during contraction and relaxation by acting as a molecular spring.

Biological function summary

The function of telethonin expands beyond simple structural support. It acts as part of the protein complex at the Z-disc important for maintaining sarcomere architecture. This complex involves interactions not only with titin but also with other structural proteins such as actin and myotilin. Telethonin's presence helps coordinate the assembly and maintenance of the Z-disc contributing to muscle strength and resilience. It also plays a role in signaling pathways that regulate muscle protein gene expression and response to mechanical stress.

Pathways

Telethonin integrates into several significant signaling cascades. One major pathway involves the titin-telethonin interaction as part of the mechanical signaling responsible for muscle function and adaptation. Through its involvement with titin telethonin influences pathways linked to muscle hypertrophy and atrophy as well as sarcomere organization. Furthermore telethonin associates with signaling proteins like myostatin an important regulator of muscle growth modulating muscle size and adaptation to workload changes.

Telethonin's malfunction often shows links with cardiomyopathies and muscular dystrophies. Mutations in the telethonin gene are associated with limb-girdle muscular dystrophy type 2G a condition where muscle weakness and degeneration occur due to defects in the muscle fiber structure. Moreover telethonin's interaction with titin connects it to dilated cardiomyopathy a condition where the heart weakens and enlarges impairing its ability to pump effectively. Understanding telethonin’s role provides insights into these muscle-related diseases and offers potential therapeutic targets for intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Muscle assembly regulating factor. Mediates the antiparallel assembly of titin (TTN) molecules at the sarcomeric Z-disk.

See full target information TCAP

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Telethonin antibody [EPR8375] - BSA and Azide free

Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (AB248591)

Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (AB248591)

Western blot - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (AB248591)

OI-RD Scanning - Anti-Telethonin antibody [EPR8375] - BSA and Azide free (AB248591)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise