Anti-TEM8/ATR antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody (AB21269)

ab21269 at 2μg/ml staining TEM8/ATR in K562 cells by ICC/IF

• WB

Supplier Data

Western blot - Anti-TEM8/ATR antibody (AB21269)

False colour image of Western blot : Anti-TEM8/ATR antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab21269 was shown to bind specifically to TEM8/ATR. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in ANTXR1 knockout cell line ab265077 (knockout cell lysate ab257350). To generate this image, wild-type and ANTXR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-TEM8/ATR antibody (ab21269) at 1 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ANTXR1 (TEM8/ATR) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-antxr1-tem8-atr-knockout-hela-cell-line-ab265077'>ab265077</a>)

Lane 2:

ANTXR1 knockout HeLa cell lysate at 20 µg

Lane 3:

SW480 cell lysate at 20 µg

Lane 4:

U-2 OS cell lysate at 20 µg

Predicted band size: 63 kDa

Observed band size: 75 kDa

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• WB

Unknown

Western blot - Anti-TEM8/ATR antibody (AB21269)

Western blot analysis of TEM8/ATR in K562 cell lysates with ab21269 at (A) 0.5, (B) 1 and (C) 2μg/ml.

Lane 1:

Western blot - Anti-TEM8/ATR antibody (ab21269) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TEM8/ATR antibody (ab21269) at 1 µg/mL

Lane 3:

Western blot - Anti-TEM8/ATR antibody (ab21269) at 2 µg/mL

All lanes:

K562 cell lysate

Predicted band size: 63 kDa

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