Rabbit Polyclonal TEM8/ATR antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ANTXR1.
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Publications
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- Angiogenesis 25:397-4102022Capillary morphogenesis gene 2 (CMG2) mediates growth factor-induced angiogenesis by regulating endothelial cell chemotaxis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLorna M Cryan,Tsz-Ming Tsang,Jessica Stiles,Lauren Bazinet,Sai Lun Lee,Samuel Garrard,Erika Madrian,Cody Roberts,Jessie Payne,Andrew Jensen,Arthur E Frankel,P Christine Ackroyd,Kenneth A Christensen,Michael S RogersPubMed 35212873
- Cells 8:2019Improved Antitumor Efficacy of Combined Vaccine Based on the Induced HUVECs and DC-CT26 Against Colorectal Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiushuang Zhang et. al.PubMed 31121964
- Matrix biology : journal of the International Society for Matrix Biology 62:105-1142016Cell autonomous ANTXR1-mediated regulation of extracellular matrix components in primary fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Hu,Bjorn R Olsen,Tatiana Y BesschetnovaPubMed 28011198
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ANTXR1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H6X2
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. (Microbial infection) Acts as a receptor for protective antigen (PA) of B.anthracis.
Alternative names
ATR, TEM8, ANTXR1, Anthrax toxin receptor 1, Tumor endothelial marker 8
Recommended products
Rabbit Polyclonal TEM8/ATR antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human ANTXR1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ANTXR1. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H6X2
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody will recognize only the largest isoform of TEM8/ATR.
- Concentration
- Purification notes
This antibody is ion exchange chromatography purified.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Western blot - Anti-TEM8/ATR antibody (ab21269)
False colour image of Western blot: Anti-TEM8/ATR antibody staining at 1 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab21269 was shown to bind specifically to TEM8/ATR. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in ANTXR1 knockout cell line Human ANTXR1 (TEM8/ATR) knockout HeLa cell line ab265077 (knockout cell lysate Human ANTXR1 (TEM8/ATR) knockout HeLa cell lysate ab257350). To generate this image, wild-type and ANTXR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-TEM8/ATR antibody (ab21269) at 1 µg/mL
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: Western blot - Human ANTXR1 (TEM8/ATR) knockout HeLa cell line (Human ANTXR1 (TEM8/ATR) knockout HeLa cell line ab265077)
Lane 2: ANTXR1 knockout HeLa cell lysate at 20 µg
Lane 3: SW480 cell lysate at 20 µg
Lane 4: U-2 OS cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 75 kDa
Western blot - Anti-TEM8/ATR antibody (ab21269)
Western blot analysis of TEM8/ATR in K562 cell lysates with ab21269 at (A) 0.5, (B) 1 and (C) 2μg/ml.
Lane 1: Western blot - Anti-TEM8/ATR antibody (ab21269) at 0.5 µg/mL
Lane 2: Western blot - Anti-TEM8/ATR antibody (ab21269) at 1 µg/mL
Lane 3: Western blot - Anti-TEM8/ATR antibody (ab21269) at 2 µg/mL
All lanes: K562 cell lysate
Predicted band size: 63 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody (ab21269)
ab21269 at 2μg/ml staining TEM8/ATR in K562 cells by ICC/IF
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