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AB246321

Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal TEM8/ATR antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.

View Alternative Names

ATR, TEM8, ANTXR1, Anthrax toxin receptor 1, Tumor endothelial marker 8

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (AB246321)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (AB246321)

Immunofluorescent analysis of 100% Methanol fixed LoVo (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in LoVo cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

PFA fixation is not recommended due to weak staining on LoVo cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241067).

Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (AB246321)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (AB246321)

Immunofluorescent analysis of 100% Methanol fixed SW480 (human colorectal adenocarcinoma epithelial cell) cells labeling TEM8/ATR with ab241067 at 1/100 dilution (Green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. Confocal image showing cytoplasmic and plasma membrane staining in SW480 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

PFA fixation is not recommended due to negative staining on SW480 cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241067).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (AB246321)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TEM8/ATR antibody [EPNCI-R173-37] - BSA and Azide free (AB246321)

Immunohistochemical analysis of 10% neutral-buffered formalin Mammary fat pad of TEM8/ATR wildtype (WT) or knockout (KO) athymic nude mice transplanted with HT29 human colon tumor tissue labeling TEM8/ATR with ab241067 at 1/60 dilution, followed by a Biotin anti-rabbit IgG secondsry antibody. Positive staining in stromal cells of transplanted HT29 human colon tumor growing in TEM8/ATR wildtype (WT) athymic nude mice is observed. Antigen retrieval : Retrieved with proteinase K. This image was kindly provided by our collaborator Dr. Brad St. Croix, National Cancer Institute (NCI), NIH, and published in J Clin Invest. 128 : 2927-2943 (PMID : 29863500). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab241067).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCI-R173-37

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

We have not yet been able to validate ab241067 for IHC-P in-house; however, our collaborator has obtained successful results. Please refer to the protocol and data available in PMID: 29863500.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>(Antigen Retrieval with proteinase K).</p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>(Antigen Retrieval with proteinase K).</p>" } } }
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Product details

ab246321 is the carrier-free version of ab241067.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.. (Microbial infection) Acts as a receptor for protective antigen (PA) of B.anthracis.
See full target information ANTXR1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:1312 PubMed38346978

2024

Residual ANTXR1+ myofibroblasts after chemotherapy inhibit anti-tumor immunity via YAP1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Licaj,Rana Mhaidly,Yann Kieffer,Hugo Croizer,Claire Bonneau,Arnaud Meng,Lounes Djerroudi,Kevin Mujangi-Ebeka,Hocine R Hocine,Brigitte Bourachot,Ilaria Magagna,Renaud Leclere,Lea Guyonnet,Mylene Bohec,Coralie Guérin,Sylvain Baulande,Maud Kamal,Christophe Le Tourneau,Fabrice Lecuru,Véronique Becette,Roman Rouzier,Anne Vincent-Salomon,Geraldine Gentric,Fatima Mechta-Grigoriou

Cancer discovery 12:1462-1481 PubMed35320348

2022

Reverse Transcriptase Inhibition Disrupts Repeat Element Life Cycle in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mihir Rajurkar,Aparna R Parikh,Alexander Solovyov,Eunae You,Anupriya S Kulkarni,Chong Chu,Katherine H Xu,Christopher Jaicks,Martin S Taylor,Connie Wu,Katherine A Alexander,Charly R Good,Annamaria Szabolcs,Stefanie Gerstberger,Antuan V Tran,Nova Xu,Richard Y Ebright,Emily E Van Seventer,Kevin D Vo,Eric C Tai,Chenyue Lu,Jasmin Joseph-Chazan,Michael J Raabe,Linda T Nieman,Niyati Desai,Kshitij S Arora,Matteo Ligorio,Vishal Thapar,Limor Cohen,Padric M Garden,Yasmeen Senussi,Hui Zheng,Jill N Allen,Lawrence S Blaszkowsky,Jeffrey W Clark,Lipika Goyal,Jennifer Y Wo,David P Ryan,Ryan B Corcoran,Vikram Deshpande,Miguel N Rivera,Martin J Aryee,Theodore S Hong,Shelley L Berger,David R Walt,Kathleen H Burns,Peter J Park,Benjamin D Greenbaum,David T Ting
View all publications
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