AB181854

Anti-TEMT antibody [EPR13240]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal TEMT antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Indolethylamine N-methyltransferase, Indolamine N-methyltransferase, Aromatic alkylamine N-methyltransferase, Thioether S-methyltransferase, Amine N-methyltransferase, Arylamine N-methyltransferase, TEMT, INMT

2 Images

Flow Cytometry (Intracellular) - Anti-TEMT antibody [EPR13240] (AB181854)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TEMT antibody [EPR13240] (AB181854)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed NCI-H2228 cells labeling TEMT with ab181854 at 1/250 dilution. Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control : Rabbit IgG.

Supplier Data

Western blot - Anti-TEMT antibody [EPR13240] (AB181854)

All lanes:

Western blot - Anti-TEMT antibody [EPR13240] (ab181854) at 1/1000 dilution

Lane 1:

Human adrenal gland cell line at 20 µg

Lane 2:

A549 cell line at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 29 kDa

false

Carrier free

Anti-TEMT antibody [EPR13240] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13240

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/250", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds (By similarity). Catalyzes the N-methylation of tryptamine and structurally related compounds.

See full target information Indolethylamine N-methyltransferase

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 13: PubMed40722696

2025

Role of BMPR2 Mutation in Lung Organoid Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Simin Jiang,Dian Chen,Liangliang Tian,Zihang Pan,Huanyu Long,Lanhe Chu,Weijing Kong,Qiyang Yao,Xiaojing Ma,Yun Zhao,Kai Wang,Yahong Chen

Cancer cell international 24:121 PubMed38555451

2024

β-hydroxybutyrate inhibits malignant phenotypes of prostate cancer cells through β-hydroxybutyrylation of indoleacetamide-N-methyltransferase.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Zhang,Yunlong Li

BMC genomics 24:127 PubMed36932340

2023

Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Zhu,Deng Qiong,Gu Yanli,Li Min,Zhang Ying,Hu Qiyi,Zhang Shenping,Wang Xisheng,Liang Hui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TEMT antibody [EPR13240]

Flow Cytometry (Intracellular) - Anti-TEMT antibody [EPR13240] (AB181854)

Western blot - Anti-TEMT antibody [EPR13240] (AB181854)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise