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AB250525

Anti-TEMT antibody [EPR13240] - BSA and Azide free

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Rabbit Recombinant Monoclonal TEMT antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Indolethylamine N-methyltransferase, Indolamine N-methyltransferase, Aromatic alkylamine N-methyltransferase, Thioether S-methyltransferase, Amine N-methyltransferase, Arylamine N-methyltransferase, TEMT, INMT

2 Images
Flow Cytometry (Intracellular) - Anti-TEMT antibody [EPR13240] - BSA and Azide free (AB250525)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TEMT antibody [EPR13240] - BSA and Azide free (AB250525)

This data was developed using ab181854, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed NCI-H2228 cells labeling TEMT with ab181854 at 1/250 dilution. Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control : Rabbit IgG.

Western blot - Anti-TEMT antibody [EPR13240] - BSA and Azide free (AB250525)
  • WB

Supplier Data

Western blot - Anti-TEMT antibody [EPR13240] - BSA and Azide free (AB250525)

This data was developed using ab181854, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TEMT antibody [EPR13240] (<a href='/en-us/products/primary-antibodies/temt-antibody-epr13240-ab181854'>ab181854</a>) at 1/1000 dilution

Lane 1:

Human adrenal gland cell line at 20 µg

Lane 2:

A549 cell line at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13240

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250525 is the carrier-free version of ab181854.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds (By similarity). Catalyzes the N-methylation of tryptamine and structurally related compounds.
See full target information Indolethylamine N-methyltransferase
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com