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Rabbit Recombinant Monoclonal TEMT antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Anti-TEMT antibody [EPR13240] - BSA and Azide free (AB250525), expandable thumbnail
  • Western blot - Anti-TEMT antibody [EPR13240] - BSA and Azide free (AB250525), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Orangutan
Predicted
Predicted
Rabbit
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Orangutan, Mouse, Rat, Rabbit

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Orangutan, Mouse, Rat, Rabbit

Dilution info

-

Notes

-

Target data

Function

Functions as thioether S-methyltransferase and is active with a variety of thioethers and the corresponding selenium and tellurium compounds, including 3-methylthiopropionaldehyde, dimethyl selenide, dimethyl telluride, 2-methylthioethylamine, 2-methylthioethanol, methyl-n-propyl sulfide and diethyl sulfide. Plays an important role in the detoxification of selenium compounds (By similarity). Catalyzes the N-methylation of tryptamine and structurally related compounds.

Alternative names

Indolethylamine N-methyltransferase, Indolamine N-methyltransferase, Aromatic alkylamine N-methyltransferase, Thioether S-methyltransferase, Amine N-methyltransferase, Arylamine N-methyltransferase, TEMT, INMT

Recommended products

Rabbit Recombinant Monoclonal TEMT antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Alternative names

Indolethylamine N-methyltransferase, Indolamine N-methyltransferase, Aromatic alkylamine N-methyltransferase, Thioether S-methyltransferase, Amine N-methyltransferase, Arylamine N-methyltransferase, TEMT, INMT

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR13240

Purification technique

Affinity purification

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab250525 is the carrier-free version of Anti-TEMT antibody [EPR13240] ab181854.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry (Intracellular) - Anti-TEMT antibody [EPR13240] - BSA and Azide free (ab250525), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TEMT antibody [EPR13240] - BSA and Azide free (ab250525)

    This data was developed using Anti-TEMT antibody [EPR13240] ab181854, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed NCI-H2228 cells labeling TEMT with Anti-TEMT antibody [EPR13240] ab181854 at 1/250 dilution. Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit IgG.

  • Western blot - Anti-TEMT antibody [EPR13240] - BSA and Azide free (ab250525), expandable thumbnail

    Western blot - Anti-TEMT antibody [EPR13240] - BSA and Azide free (ab250525)

    This data was developed using Anti-TEMT antibody [EPR13240] ab181854, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-TEMT antibody [EPR13240] (Anti-TEMT antibody [EPR13240] ab181854) at 1/1000 dilution

    Lane 1: Human adrenal gland cell line at 20 µg

    Lane 2: A549 cell line at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 29 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com